{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:37:47Z","timestamp":1760150267691,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"],"award-info":[{"award-number":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"China Agriculture Research System of MOF and MARA","award":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"],"award-info":[{"award-number":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"]}]},{"name":"Agricultural Science and Technology Innovation Program of China","award":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"],"award-info":[{"award-number":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"]}]},{"name":"General Project of Shanxi Province Basic Research Program","award":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"],"award-info":[{"award-number":["32172704","CARS-38","CAAS-ZDRW202106","ASTIP-IAS13","20210302123371"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>MicroRNA (miRNA) is a type of endogenous short\u2212stranded ncRNA that influences many biological processes such as animal growth, development and metabolism. The thyroid gland is an important endocrine gland in sheep, and an increasing number of studies have shown that the thyroid gland plays an important role in animal reproduction, but the molecular mechanisms of the thyroid gland in sheep reproduction are poorly understood. In this study, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between the follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) small-tail Han (STH) sheep, respectively, and to identify differentially expressed miRNAs (DEMs) associated with reproduction. Bioinformatic analysis of the target genes of these DEMs revealed that they can be enriched in multiple GO terms associated with the reproductive process in animals and in the KEGG signaling pathway. The miRNA\u2013mRNA coexpression network revealed that oar-miR-133 and oar-miR-370-3p may play an important role in sheep reproduction. The results of the dual-luciferase reporter assay suggest a possible targeting relationship between novel-51 and TARBP2. These results provided a novel resource for elucidating regulatory mechanisms underlying STH sheep prolificacy.<\/jats:p>","DOI":"10.3390\/genes14112024","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:50:42Z","timestamp":1698670242000},"page":"2024","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes"],"prefix":"10.3390","volume":"14","author":[{"given":"Cheng","family":"Chang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"},{"name":"College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2159-5635","authenticated-orcid":false,"given":"Xiaoyun","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3450-738X","authenticated-orcid":false,"given":"Ran","family":"Di","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7375-8937","authenticated-orcid":false,"given":"Xiangyu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaoceng","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4510-2014","authenticated-orcid":false,"given":"Chen","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5164-0310","authenticated-orcid":false,"given":"Mingxing","family":"Chu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cheng, S., Wang, X., Zhang, Q., He, Y., Zhang, X., Yang, L., and Shi, J. 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