{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:29:16Z","timestamp":1773793756566,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Zhejiang Province, China","award":["LY23C140003"],"award-info":[{"award-number":["LY23C140003"]}]},{"name":"Natural Science Foundation of Zhejiang Province, China","award":["2020-J-005-ydhz"],"award-info":[{"award-number":["2020-J-005-ydhz"]}]},{"name":"Research and demonstration of key technologies for ensuring supply of vegetable industry","award":["LY23C140003"],"award-info":[{"award-number":["LY23C140003"]}]},{"name":"Research and demonstration of key technologies for ensuring supply of vegetable industry","award":["2020-J-005-ydhz"],"award-info":[{"award-number":["2020-J-005-ydhz"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>In higher plants, WRKY transcription factors are broadly involved in a variety of life activities and play an important role in both biotic and abiotic stress responses. However, little is known about the functions of WRKY genes in the popular species, such as Cucurbita maxima (pumpkin), which is planted worldwide. In the present study, 102 CmWRKY genes were identified in the C. maxima genome. Chromosome location, multiple sequence alignment, phylogenetic analysis, and synteny analysis of the CmWRKYs were performed. Notably, we found that silencing CmWRKY22 promoted cucumber mosaic virus (CMV) infection, whereas overexpression of CmWRKY22 inhibited the CMV infection. Subsequently, an electrophoretic mobility shift assay (EMSA) confirmed that CmWRKY22 was able to bind to the W-box at the promoter of CmPR1b, which is a responsive gene of the salicylic acid (SA) signaling pathway. In summary, this study has provided a foundation for the antiviral functions of WRKY transcription factors in C. maxima.<\/jats:p>","DOI":"10.3390\/genes14112030","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T12:44:03Z","timestamp":1698756243000},"page":"2030","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genome-Wide Identification and Characterization of the WRKY Gene Family in Cucurbita maxima"],"prefix":"10.3390","volume":"14","author":[{"given":"Qin","family":"Zhou","sequence":"first","affiliation":[{"name":"Jinhua Academy of Agricultural Sciences, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziqing","family":"Guo","sequence":"additional","affiliation":[{"name":"Jinhua Academy of Agricultural Sciences, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jinhua Academy of Agricultural Sciences, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Melons and Vegetables Germplasm Resource Innovation and Intelligent Technology, Hefei 230031, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duanhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Hunan Vegetable Research Institute, Changsha 410125, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailiang","family":"Bo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8428-385X","authenticated-orcid":false,"given":"Pu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jinhua Academy of Agricultural Sciences, Jinhua 321000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1093\/jxb\/ert422","article-title":"Transcription factor-mediated cell-to-cell signalling in plants","volume":"65","author":"Han","year":"2014","journal-title":"J. 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