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(CNPq)","doi-asserted-by":"publisher","award":["301978\/2022-0"],"award-info":[{"award-number":["301978\/2022-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["2022\/10775-9"],"award-info":[{"award-number":["2022\/10775-9"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["UID\/AMB\/50017\/2019"],"award-info":[{"award-number":["UID\/AMB\/50017\/2019"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["2018\/05522-9"],"award-info":[{"award-number":["2018\/05522-9"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","award":["301978\/2022-0"],"award-info":[{"award-number":["301978\/2022-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Coffee plants have been targeted by a devastating bacterial disease, a condition known as bacterial blight, caused by the phytopathogen Pseudomonas syringae pv. garcae (Psg). Conventional treatments of coffee plantations affected by the disease involve frequent spraying with copper- and kasugamycin-derived compounds, but they are both highly toxic to the environment and stimulate the appearance of bacterial resistance. Herein, we report the molecular characterization and mechanical features of the genome of two newly isolated (putative polyvalent) lytic phages for Psg. The isolated phages belong to class Caudoviricetes and present a myovirus-like morphotype belonging to the genuses Tequatrovirus (PsgM02F) and Phapecoctavirus (PsgM04F) of the subfamilies Straboviridae (PsgM02F) and Stephanstirmvirinae (PsgM04F), according to recent bacterial viruses\u2019 taxonomy, based on their complete genome sequences. The 165,282 bp (PsgM02F) and 151,205 bp (PsgM04F) genomes do not feature any lysogenic-related (integrase) genes and, hence, can safely be assumed to follow a lytic lifestyle. While phage PsgM02F produced a morphogenesis yield of 124 virions per host cell, phage PsgM04F produced only 12 virions per host cell, indicating that they replicate well in Psg with a 50 min latency period. Genome mechanical analyses established a relationship between genome bendability and virion morphogenesis yield within infected host cells.<\/jats:p>","DOI":"10.3390\/genes15010113","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T06:41:22Z","timestamp":1705560082000},"page":"113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization and Genome Mechanical Features of Two Newly Isolated Polyvalent Bacteriophages Infecting Pseudomonas syringae pv. garcae"],"prefix":"10.3390","volume":"15","author":[{"given":"Erica C.","family":"Silva","sequence":"first","affiliation":[{"name":"VBlab\u2014Laboratory of Bacterial Viruses, University of Sorocaba, Sorocaba 18023-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9119-395X","authenticated-orcid":false,"given":"Carlos A.","family":"Quinde","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basilio","family":"Cieza","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aakash","family":"Basu","sequence":"additional","affiliation":[{"name":"Department of Biosciences, Durham University, Durham DH1 3LE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta M. D. C.","family":"Vila","sequence":"additional","affiliation":[{"name":"VBlab\u2014Laboratory of Bacterial Viruses, University of Sorocaba, Sorocaba 18023-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0772-2834","authenticated-orcid":false,"given":"Victor M.","family":"Balc\u00e3o","sequence":"additional","affiliation":[{"name":"VBlab\u2014Laboratory of Bacterial Viruses, University of Sorocaba, Sorocaba 18023-000, SP, Brazil"},{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, P-3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/s41559-018-0793-y","article-title":"The global burden of pathogens and pests on major food crops","volume":"3","author":"Savary","year":"2019","journal-title":"Nat. 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