{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:44:54Z","timestamp":1760143494783,"version":"build-2065373602"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea (NRF)","doi-asserted-by":"publisher","award":["NRF-2021R1I1A1A01050945","2022R1F1A1064169","HR22C1605"],"award-info":[{"award-number":["NRF-2021R1I1A1A01050945","2022R1F1A1064169","HR22C1605"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute (KHIDI)","doi-asserted-by":"publisher","award":["NRF-2021R1I1A1A01050945","2022R1F1A1064169","HR22C1605"],"award-info":[{"award-number":["NRF-2021R1I1A1A01050945","2022R1F1A1064169","HR22C1605"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The primary goal of this investigation was to identify mRNA targets affected by dysregulated miRNAs in RIF. This was accomplished by comprehensively analyzing mRNA and miRNA expression profiles in two groups: female subjects with normal reproductive function (control, n = 5) and female subjects experiencing recurrent implantation failure (RIF, n = 5). We conducted transcriptome sequencing and small RNA sequencing on endometrial tissue samples from these cohorts. Subsequently, we validated a selection of intriguing findings using real-time PCR with samples from the same cohort. In total, our analysis revealed that 929 mRNAs exhibited differential expression patterns between the control and RIF patient groups. Notably, our investigation confirmed the significant involvement of dysregulated genes in the context of RIF. Furthermore, we uncovered promising correlation patterns within these mRNA\/miRNA pairs. Functional categorization of these miRNA\/mRNA pairs highlighted that the differentially expressed genes were predominantly associated with processes such as angiogenesis and cell adhesion. We identified new target genes that are regulated by miR-665, including Blood Vessel Epicardial Substance (BVES) and Adenosylhomocysteinase like 2 (AHCYL2). Our findings suggest that abnormal regulation of genes involved in angiogenesis and cell adhesion, including BVES and AHCYL2, contributes to the endometrial dysfunction observed in women with recurrent implantation failure (RIF) compared to healthy women.<\/jats:p>","DOI":"10.3390\/genes15020244","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T03:57:29Z","timestamp":1707969449000},"page":"244","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR-665-Mediated Regulation of AHCYL2 and BVES Genes in Recurrent Implantation Failure"],"prefix":"10.3390","volume":"15","author":[{"given":"Sung Hwan","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea"},{"name":"College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8326-7768","authenticated-orcid":false,"given":"Young Myeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Kangwon Institute of Inclusive Technology, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui Jeong","family":"An","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea"},{"name":"College of Life Science, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9864-9777","authenticated-orcid":false,"given":"Ji Hyang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13496, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0541-3528","authenticated-orcid":false,"given":"Nam Keun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.fertnstert.2023.02.014","article-title":"Recurrent implantation failure: Reality or a statistical mirage?: Consensus statement from the July 1, 2022 Lugano Workshop on recurrent implantation failure","volume":"120","author":"Pirtea","year":"2023","journal-title":"Fertil. 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