{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T15:27:30Z","timestamp":1785857250681,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Leibniz Collaborative Excellence program","award":["K52\/2017"],"award-info":[{"award-number":["K52\/2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Since artificial insemination is common practice in pig breeding, the quality and persistence of the semen are decisive for the usability of individual boars. In the current study, genome-wide association analyses were performed to investigate the genetic variability underlying phenotypic variations in semen characteristics. These traits comprise sperm morphology and sperm motility under different temporal and thermal storage conditions, in addition to standard semen quality parameters. Two consecutive samples of the fourth and fifth ejaculates from the same boar were comprehensively analyzed in a genotyped Pi\u00e9train boar population. A total of 13 genomic regions on different chromosomes were identified that contain single-nucleotide polymorphisms significantly associated with these traits. Subsequent analysis of the genomic regions revealed candidate genes described to be involved in spermatogenesis, such as FOXL3, GPER1, PDGFA, PRKAR1B, SNRK, SUN1, and TSPO, and sperm motility, including ARRDC4, CEP78, DNAAF5, and GPER1. Some of these genes were also associated with male fertility or infertility in mammals (e.g., CEP78, GPER1). The analyses based on these laboriously determined and valuable phenotypes contribute to a better understanding of the genetic background of male fertility traits in pigs and could prospectively contribute to the improvement of sperm quality through breeding approaches.<\/jats:p>","DOI":"10.3390\/genes15030382","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T10:44:33Z","timestamp":1710931473000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Genome-Wide Association Analysis of Semen Characteristics in Pi\u00e9train Boars"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6470-0434","authenticated-orcid":false,"given":"Henry","family":"Reyer","sequence":"first","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Abou-Soliman","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"},{"name":"Department of Animal and Poultry Breeding, Desert Research Center, Cairo 11753, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7932-9412","authenticated-orcid":false,"given":"Martin","family":"Schulze","sequence":"additional","affiliation":[{"name":"Institute for Reproduction of Farm Animals Sch\u00f6now, 16321 Bernau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Henne","sequence":"additional","affiliation":[{"name":"BHZP GmbH, 21368 Dahlenburg-Ellringen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Reinsch","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Schoen","sequence":"additional","affiliation":[{"name":"Leibniz Institute for Zoo and Wildlife Research (IZW), 10315 Berlin, Germany"},{"name":"Institute of Biotechnology, Technische Universit\u00e4t Berlin, 10623 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9523-6790","authenticated-orcid":false,"given":"Klaus","family":"Wimmers","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"},{"name":"Faculty of Agricultural and Environmental Sciences, University of Rostock, 18059 Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/age.12133","article-title":"Genome-wide association analysis for growth, muscularity and meat quality in Pi\u00e9train pigs","volume":"45","author":"Stratz","year":"2014","journal-title":"Anim. 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