{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:08:04Z","timestamp":1790777284808,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,7]],"date-time":"2024-04-07T00:00:00Z","timestamp":1712448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Human sperm parameters serve as a first step in diagnosing male infertility, but not in determining the potential for successful pregnancy during assisted reproductive technologies (ARTs) procedures. Here, we investigated the relationship between sperm head morphology at high magnification, based on strict morphologic criteria, and the nuclear architecture analyzed by fluorescence in situ hybridization (FISH). We included five men. Two of them had an elevated high-magnification morphology score of 6 points (Score 6) indicating high fertility potential, whereas three had a low score of 0 points (Score 0), indicating low fertility potential. We used FISH to study the inter-telomeric distance and the chromosomal territory area of chromosome 1 (Chr. 1). We then compared these two parameters between subjects with high and low scores. FISH data analysis showed that the inter-telomeric distance (ITD) and chromosomal territory area (CTA) of Chr. 1 were significantly higher in subjects with low scores (score 0) than high scores (score 6). Our results suggest that (i) there is a link between nuclear architecture and sperm head abnormalities, particularly vacuoles; and (ii) it is possible to select spermatozoa with normal nuclear architecture, which might indirectly explain the positive ART outcomes observed with this technique.<\/jats:p>","DOI":"10.3390\/genes15040464","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T10:11:55Z","timestamp":1712571115000},"page":"464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Different Nuclear Architecture in Human Sperm According to Their Morphology"],"prefix":"10.3390","volume":"15","author":[{"given":"Nino-Guy","family":"Cassuto","sequence":"first","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nesrine","family":"Ogal","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Armand-Trousseau Hospital, AP-HP, INSERM Unit U933, Sorbonne University, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6448-7995","authenticated-orcid":false,"given":"Said","family":"Assou","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine and Biotherapy, University Montpellier, INSERM, CHU Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Ruoso","sequence":"additional","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli-Jonathan","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Armand-Trousseau Hospital, AP-HP, INSERM Unit U933, Sorbonne University, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel-Jos\u00e9","family":"Monteiro","sequence":"additional","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Thomas","sequence":"additional","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Siffroi","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Armand-Trousseau Hospital, AP-HP, INSERM Unit U933, Sorbonne University, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Rouen","sequence":"additional","affiliation":[{"name":"Maternity of Bluets, Medically Assisted Reproduction Service, 75012 Paris, France"},{"name":"AP-HP, H\u00f4tel-Dieu, Sleep and Vigilance Center, Universit\u00e9 Paris Cit\u00e9, VIFASOM, ERC 7330, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/S0140-6736(78)92957-4","article-title":"Birth after the Reimplantation of a Human Embryo","volume":"2","author":"Steptoe","year":"1978","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0140-6736(92)92425-F","article-title":"Pregnancies after Intracytoplasmic Injection of Single Spermatozoon into an Oocyte","volume":"340","author":"Palermo","year":"1992","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Baldini, D., Ferri, D., Baldini, G.M., Lot, D., Catino, A., Vizziello, D., and Vizziello, G. 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