{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:35:14Z","timestamp":1769848514340,"version":"3.49.0"},"reference-count":50,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"major projects in agricultural biology breeding","award":["2023ZD0406401"],"award-info":[{"award-number":["2023ZD0406401"]}]},{"name":"major projects in agricultural biology breeding","award":["X220981UZ230"],"award-info":[{"award-number":["X220981UZ230"]}]},{"name":"major projects in agricultural biology breeding","award":["2020A01"],"award-info":[{"award-number":["2020A01"]}]},{"name":"major projects in agricultural biology breeding","award":["2019KCXTD006"],"award-info":[{"award-number":["2019KCXTD006"]}]},{"DOI":"10.13039\/100017130","name":"Ji Hua Laboratory","doi-asserted-by":"publisher","award":["2023ZD0406401"],"award-info":[{"award-number":["2023ZD0406401"]}],"id":[{"id":"10.13039\/100017130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017130","name":"Ji Hua Laboratory","doi-asserted-by":"publisher","award":["X220981UZ230"],"award-info":[{"award-number":["X220981UZ230"]}],"id":[{"id":"10.13039\/100017130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017130","name":"Ji Hua Laboratory","doi-asserted-by":"publisher","award":["2020A01"],"award-info":[{"award-number":["2020A01"]}],"id":[{"id":"10.13039\/100017130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017130","name":"Ji Hua Laboratory","doi-asserted-by":"publisher","award":["2019KCXTD006"],"award-info":[{"award-number":["2019KCXTD006"]}],"id":[{"id":"10.13039\/100017130","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Qingyuan Science and Technology project of Qingcheng District, Qingyuan City","award":["2023ZD0406401"],"award-info":[{"award-number":["2023ZD0406401"]}]},{"name":"Qingyuan Science and Technology project of Qingcheng District, Qingyuan City","award":["X220981UZ230"],"award-info":[{"award-number":["X220981UZ230"]}]},{"name":"Qingyuan Science and Technology project of Qingcheng District, Qingyuan City","award":["2020A01"],"award-info":[{"award-number":["2020A01"]}]},{"name":"Qingyuan Science and Technology project of Qingcheng District, Qingyuan City","award":["2019KCXTD006"],"award-info":[{"award-number":["2019KCXTD006"]}]},{"name":"Poultry Breeding Precision Innovation Team","award":["2023ZD0406401"],"award-info":[{"award-number":["2023ZD0406401"]}]},{"name":"Poultry Breeding Precision Innovation Team","award":["X220981UZ230"],"award-info":[{"award-number":["X220981UZ230"]}]},{"name":"Poultry Breeding Precision Innovation Team","award":["2020A01"],"award-info":[{"award-number":["2020A01"]}]},{"name":"Poultry Breeding Precision Innovation Team","award":["2019KCXTD006"],"award-info":[{"award-number":["2019KCXTD006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Eggshell color plays important biological roles and attracts the attention of both egg retailers and researchers. However, whether non-coding RNAs are involved in pigment deposition among different eggshell colors remains unknown. In this study, RNA sequencing was used to analyse the uterine gland transcriptome (CircRNA and miRNA) of Changshun chicken blue-shell hens producing four different eggshell color eggs including dark blue PK(DB) and light blue (LB), dark brown and greenish (between blue and pink, DP) and pink (p). We found that miR-192-x, targeting SLC16a7, was expressed in DB, DP, and LB groups compared with the PK group, which indicates that miR-192-x may play a role in the blue eggshell color. KEGG and GO analyses showed that the \u201cmetabolic pathways\u201d with targeted genes such BLVRA and HMOX1 were detected in dark and light blue color eggshell chickens, which confirms the different ratios of biliverdin and HO-1 involved in the deposition of blue color. As annotated by connectivity analysis, RASGRF1 and RASGRF2, belonging to the RASGRF family, are involved in the Ras signaling pathway, which plays an important role in cell growth, differentiation, metastasis and apoptosis. Our findings enrich the database of circRNA, miRNAs and genes for chicken uterine tissue, which will be useful in accelerating molecular selection for blue eggshell color layers.<\/jats:p>","DOI":"10.3390\/genes15060812","type":"journal-article","created":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T05:27:19Z","timestamp":1718861239000},"page":"812","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Network of Circular RNA and MicroRNA Sequencing Provides Insights into Pigment Deposition of Changshun Blue Eggshell Chickens"],"prefix":"10.3390","volume":"15","author":[{"given":"Siyu","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengqiao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kecheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaming","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan 528231, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, Z., Meng, G., Bai, Y., Liu, R., Du, Y., and Su, L. 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