{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T04:36:00Z","timestamp":1786250160053,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Natural Science Foundation of Fujian Province","award":["2022J05243"],"award-info":[{"award-number":["2022J05243"]}]},{"name":"the Natural Science Foundation of Fujian Province","award":["JAT210334"],"award-info":[{"award-number":["JAT210334"]}]},{"name":"the Natural Science Foundation of Fujian Province","award":["33004310"],"award-info":[{"award-number":["33004310"]}]},{"name":"the Young and Middle-aged Teachers Education Scientific Research Project of Fujian Provincial Department of Education","award":["2022J05243"],"award-info":[{"award-number":["2022J05243"]}]},{"name":"the Young and Middle-aged Teachers Education Scientific Research Project of Fujian Provincial Department of Education","award":["JAT210334"],"award-info":[{"award-number":["JAT210334"]}]},{"name":"the Young and Middle-aged Teachers Education Scientific Research Project of Fujian Provincial Department of Education","award":["33004310"],"award-info":[{"award-number":["33004310"]}]},{"name":"the Minjiang University Introduces Talent pre-research project","award":["2022J05243"],"award-info":[{"award-number":["2022J05243"]}]},{"name":"the Minjiang University Introduces Talent pre-research project","award":["JAT210334"],"award-info":[{"award-number":["JAT210334"]}]},{"name":"the Minjiang University Introduces Talent pre-research project","award":["33004310"],"award-info":[{"award-number":["33004310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Abalone is a popular mollusk in the marine aquaculture industry of China. However, existing challenges, like slow growth, individual miniaturization, and the absence of abundant abalone, have emerged as significant obstacles impeding its long-term progress in aquaculture. Studies have demonstrated that insulin-related peptide (IRP) is a crucial factor in the growth of marine organisms. However, limited studies have been conducted on IRP in abalone. This study indicated that the hdh-MIRP1 open reading frame (ORF) was composed of 456 base pairs, which encoded 151 amino acids. Based on the gene expression and immunofluorescence analyses, the cerebral ganglion of Haliotis discus hannai (H. discus hannai) was the primary site of hdh-MIRP1 mRNA expression. Moreover, hdh-MIRP1 expression was observed to be higher in the larger group than in the smaller group abalones. Only single nucleotide polymorphism (SNP) was related to their growth characteristics. However, approximately 82 proteins that may interact with hdh-MIRP1 were identified. The functional enrichment analysis of the 82 genes indicated that hdh-MIRP1 may be involved in the regulation of glucose metabolism and the process of growth. This study established a benchwork for further investigating the role of IRP in the growth of abalone.<\/jats:p>","DOI":"10.3390\/genes15070960","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T15:47:32Z","timestamp":1721836052000},"page":"960","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Cloning, Characterization, and Function of Insulin-Related Peptide 1 (IRP1) in the Haliotis discus hanna"],"prefix":"10.3390","volume":"15","author":[{"given":"Jianfang","family":"Huang","sequence":"first","affiliation":[{"name":"Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China"},{"name":"State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcan","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihuan","family":"Ke","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.2983\/035.032.0117","article-title":"Estimates of correlations for shell morphological traits on body weight of interspecific hybrid abalone (Haliotis discus hannai and Haliotis gigantea)","volume":"32","author":"Luo","year":"2013","journal-title":"J. Shellfish Res."},{"key":"ref_2","unstructured":"Wang, D., and Wu, F.X. (2022). China Fishery Statistical Yearbook, China Agriculture Press."},{"key":"ref_3","unstructured":"Dong, Y. (2012). Transcriptome Analysis using 454 Pyrosequencing and Cloning and Expression of Growth-Related Genes for the Blood Clam Tegillarca granosa (Linnaeus, 1758). [Ph.D. Thesis, Ocean University of China]."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hao, R., Zheng, Z., Du, X., Wang, Q., Li, J., Deng, Y., and Chen, W. (2018). Molecular cloning and characteristics analysis of Pmtgfbr1 from Pinctada fucata martensii. Biotechnol. Rep. Amst., 19.","DOI":"10.1016\/j.btre.2018.e00262"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"736926","DOI":"10.1016\/j.aquaculture.2021.736926","article-title":"A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas","volume":"543","author":"He","year":"2021","journal-title":"Aquaculture"},{"key":"ref_6","unstructured":"Liu, G. (2013). The Roles of BMP\/Smad Pathway and Tyrosinase in the Early Development of the Pacific Oyster Crassostrea gigas. [Master\u2019s Thesis, Institute of Oceanology, The University of Chinese Academy of Sciences]."},{"key":"ref_7","unstructured":"He, P.R. (2015). IRR and FGFR Genes in Haliotis diversicolor: Molecular Cloning, Expression Analysis and Identification of SNPs Associated with Growth Traits. [Master\u2019s Thesis, Xiamen University]."},{"key":"ref_8","unstructured":"Ai, J.L. (2015). Molecular Cloning, Expression Analysis of the Gene MSTN, BMP-2 and SNPs Association with Growth Traits in Haliotis diversicolor Supertexta. [Master\u2019s Thesis, Ocean University of Guangdong]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.aggene.2016.07.004","article-title":"RNA interference in Haliotis rufescens myostatin evidences upregulation of insulin signaling pathway","volume":"1","author":"Molina","year":"2016","journal-title":"Agri Gene"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.aquaculture.2011.12.019","article-title":"Differential expression of neuropeptides correlates with growth rate in cultivated Haliotis asinina (Vetigastropoda: Mollusca)","volume":"334","author":"York","year":"2012","journal-title":"Aquaculture"},{"key":"ref_11","unstructured":"Wang, F. (2016). Research of Development Transcriptome Library and Function of Insulin-like Peptide Genes in Sinonovacula constricta. [Master\u2019s Thesis, Ocean University of Shanghai]."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"R935","DOI":"10.1152\/ajpregu.00459.2003","article-title":"Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout","volume":"286","author":"Castillo","year":"2004","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.cbpb.2019.03.002","article-title":"Nonadditive and allele-specific expression of insulin-like growth factor 1 in Nile tilapia (Oreochromis niloticus, \u2640) \u00d7 blue tilapia (O. aureus, \u2642) hybrids","volume":"232","author":"Zhou","year":"2019","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/eea.12981","article-title":"Drosophila insulin-like peptides: From expression to functions\u2013a review","volume":"169","author":"Semaniuk","year":"2020","journal-title":"Entomol. Exp. Appl."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/0306-4522(95)00378-9","article-title":"Expression and characterization of molluscan insulin-related peptide VII from the mollusc Lymnaea stagnalis","volume":"70","author":"Smit","year":"1996","journal-title":"Neuroscience"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1038\/s41598-019-57329-3","article-title":"The role of a new insulin-like peptide in the pearl oyster Pinctada fucata martensii","volume":"10","author":"Zhang","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1196\/annals.1327.091","article-title":"Pleiotropic action of insulin-like peptides of mollusk, Anodonta cygnea","volume":"1040","author":"Shipilov","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.fsi.2016.03.012","article-title":"Identification, expression, and innate immune responses of two insulin-like peptide genes in the razor clam Sinonovacula constricta","volume":"51","author":"Niu","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ygcen.2018.10.019","article-title":"Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening","volume":"271","author":"Adeline","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"145244","DOI":"10.1016\/j.gene.2020.145244","article-title":"Identification, characterization, and expression profiles of insulin-like peptides suggest their critical roles in growth regulation of the Pacific oyster, Crassostrea gigas","volume":"769","author":"Li","year":"2021","journal-title":"Gene"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bse.2012.09.008","article-title":"Polymorphism in the insulin-related peptide gene and its association with growth traits in the Pacific oyster Crassostrea gigas","volume":"46","author":"Cong","year":"2013","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.ygcen.2014.01.012","article-title":"An insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab Callinectes sapidus","volume":"199","author":"Chung","year":"2014","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fsi.2019.02.053","article-title":"Interaction of the small GTP-binding protein (Rab7) with \u03b2-actin in Litopenaeus vannamei and its role in white spot syndrome virus infection","volume":"88","author":"Feng","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_24","unstructured":"Xie, S.M. (2018). Expression Characteristics and Preliminary Functional Analysis of Three Genes in the Insulin-like Growth Factor System of Razor Clam Sinonovacula constricta. [Ph.D. Thesis, Ocean University of Shanghai]."},{"key":"ref_25","unstructured":"Chen, X.T. (2016). Characterization of the Insulin-like Peptides Gene in Bombyx Mori (BmILP). [Master\u2019s Thesis, Jiangsu University]."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kang, S.M., Tark, D., Song, B.M., Lee, G.H., Yang, J.H., Han, H.J., and Yim, S.K. (2022). Evaluation of Antiviral Effect against SARS-CoV-2 Propagation by Crude Polysaccharides from Seaweed and Abalone Viscera In Vitro. Mar. Drugs, 20.","DOI":"10.3390\/md20050296"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.ygcen.2010.02.010","article-title":"Neurohormones and neuropeptides encoded by the genome of Lottia gigantea, with reference to other mollusks and insects","volume":"167","author":"Veenstra","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0301-0082(97)00063-4","article-title":"Towards understanding the role of insulin in the brain: Lessons from insulin-related signaling systems in the invertebrate brain","volume":"54","author":"Smit","year":"1998","journal-title":"Prog. Neurobiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1677\/joe.1.06284","article-title":"cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression","volume":"187","author":"Hamano","year":"2005","journal-title":"J. Endocrinol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0960-9822(01)00068-9","article-title":"An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control","volume":"11","author":"Brogiolo","year":"2001","journal-title":"Curr. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/S0196-9781(01)00666-0","article-title":"Insulin-related peptides and their conserved signal transduction pathway","volume":"23","author":"Claeys","year":"2002","journal-title":"Peptides"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0303-7207(98)00057-4","article-title":"IGF-I in the bony fish Cottus scorpius: cDNA, expression and differential localization in brain and islets","volume":"141","author":"Schmid","year":"1998","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.ygcen.2019.05.006","article-title":"Molecular and functional analysis of the insulin-like peptides gene in the oriental river prawn Macrobrachium nipponense","volume":"280","author":"Li","year":"2019","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/jez.b.21383","article-title":"Developmental transcriptome of Aplysia californica","volume":"316b","author":"Heyland","year":"2011","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1007\/BF01928165","article-title":"The Light Green Cells of Lymnaea: A neuroendocrine model system for stimulus-induced expression of multiple peptide genes in a single cell type","volume":"48","author":"Geraerts","year":"1992","journal-title":"Experientia"},{"key":"ref_36","first-page":"19","article-title":"Proteins interacting with chicken NOS2 were screened by GST pull-down combined with mass spectrometry","volume":"42","author":"Zhou","year":"2020","journal-title":"China Poult."},{"key":"ref_37","unstructured":"Wang, X.L. (2018). Cloning, Expression and Functional Characterization of Superoxide Dismutase Multigene Family in Nibea albiflora. [Master\u2019s Thesis, Jimei University]."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/bab.1714","article-title":"Employment of fucosidases for the synthesis of fucosylated oligosaccharides with biological potential","volume":"66","year":"2019","journal-title":"Biotechnol. Appl. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1046\/j.1432-0436.2000.660203.x","article-title":"Down regulation of ribosomal protein mRNAs during neuronal differentiation of human NTERA2 cells","volume":"66","author":"Leffers","year":"2000","journal-title":"Differentiation"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"429","DOI":"10.3109\/08830189909088492","article-title":"Ribosomal proteins in cell proliferation and apoptosis","volume":"18","author":"Chen","year":"1999","journal-title":"Int. Rev. Immunol."},{"key":"ref_41","unstructured":"Qian, Z.Y. (2014). Isolation, Identification, Expression and Function Studies of Growth Performance Candidate Genes in Litopenaeus vannamei. [Ph.D Thesis, Northwest A & F University]."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1007\/s11427-016-0018-0","article-title":"The role of ribosomal proteins in the regulation of cell proliferation, tumorigenesis, and genomic integrity","volume":"59","author":"Xu","year":"2016","journal-title":"Sci. China Life Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1038\/s41580-019-0123-5","article-title":"Metabolic regulation of cell growth and proliferation","volume":"20","author":"Zhu","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ygcen.2017.01.016","article-title":"Effects of glucose and insulin administration on glucose transporter expression in the North Pacific spiny dogfish (Squalus suckleyi)","volume":"247","author":"Deck","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.peptides.2015.02.003","article-title":"Regulation of hemolymph trehalose level by an insulin-like peptide through diel feeding rhythm of the beet armyworm, Spodoptera exigua","volume":"68","author":"Kim","year":"2015","journal-title":"Peptides"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1023\/B:FISH.0000032726.78074.04","article-title":"Insights into insulin and glucagon responses in fish","volume":"27","author":"Navarro","year":"2002","journal-title":"Fish Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/414799a","article-title":"Insulin signalling and the regulation of glucose and lipid metabolism","volume":"414","author":"Saltiel","year":"2001","journal-title":"Nature"},{"key":"ref_48","first-page":"105","article-title":"Gene structure and expression analysis of insulin-like peptide in the Pacific white shrimp Litopenaeus vannamei","volume":"5","author":"Su","year":"2024","journal-title":"Prog. Fish. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Mohd Nor, N.A., Budin, S.B., Zainalabidin, S., Jalil, J., Sapian, S., Jubaidi, F.F., and Mohamad Anuar, N.N. (2022). The Role of Polyphenol in Modulating Associated Genes in Diabetes-Induced Vascular Disorders. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126396"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/15\/7\/960\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:20:52Z","timestamp":1760109652000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/15\/7\/960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,22]]},"references-count":49,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["genes15070960"],"URL":"https:\/\/doi.org\/10.3390\/genes15070960","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,22]]}}}