{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:19:04Z","timestamp":1760145544830,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T00:00:00Z","timestamp":1722124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["24-14-20015"],"award-info":[{"award-number":["24-14-20015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>A genome of Rhodococcus\u00a0rhodochrous IEGM 1362 was sequenced and annotated. This strain can transform monoterpene alcohol (\u2013)-isopulegol with the formation of two novel pharmacologically promising metabolites. Nine genes encoding cytochrome P450, presumably involved in (\u2013)-isopulegol transformation, were found in the genome of R.\u00a0rhodochrous IEGM 1362. Primers and PCR conditions for their detection were selected. The obtained data can be used for the further investigation of genes encoding enzymes involved in monoterpene biotransformation.<\/jats:p>","DOI":"10.3390\/genes15080992","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T15:26:53Z","timestamp":1722526013000},"page":"992","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bioinformatics Analysis of the Genome of Rhodococcus\u00a0rhodochrous IEGM 1362, an (\u2212)-Isopulegol Biotransformer"],"prefix":"10.3390","volume":"15","author":[{"given":"Polina Yu.","family":"Maltseva","sequence":"first","affiliation":[{"name":"Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia"},{"name":"Department of Microbiology and Immunology, Perm State University, 15 Bukirev Str., 614990 Perm, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9292-5726","authenticated-orcid":false,"given":"Natalia A.","family":"Plotnitskaya","sequence":"additional","affiliation":[{"name":"Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia"},{"name":"Department of Microbiology and Immunology, Perm State University, 15 Bukirev Str., 614990 Perm, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3788-217X","authenticated-orcid":false,"given":"Anastasiia V.","family":"Krivoruchko","sequence":"additional","affiliation":[{"name":"Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia"},{"name":"Department of Microbiology and Immunology, Perm State University, 15 Bukirev Str., 614990 Perm, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksey V.","family":"Beletskiy","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 7-1 Prosp. 60-let Oktyabrya, 117312 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey L.","family":"Rakitin","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 7-1 Prosp. 60-let Oktyabrya, 117312 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8245-8757","authenticated-orcid":false,"given":"Andrey V.","family":"Mardanov","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 7-1 Prosp. 60-let Oktyabrya, 117312 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2558-4789","authenticated-orcid":false,"given":"Irina B.","family":"Ivshina","sequence":"additional","affiliation":[{"name":"Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia"},{"name":"Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 7-1 Prosp. 60-let Oktyabrya, 117312 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e202100194","DOI":"10.1002\/tcr.202100194","article-title":"Enantiomeric Isopulegol as the Chiral Pool in the Total Synthesis of Bioactive Agents","volume":"22","author":"Szakonyi","year":"2022","journal-title":"Chem. 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