{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:20:07Z","timestamp":1760145607415,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PRIN-2022 PNRR project","award":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"],"award-info":[{"award-number":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"]}]},{"name":"CNR project","award":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"],"award-info":[{"award-number":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"]}]},{"name":"BANCA D\u2019ITALIA","award":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"],"award-info":[{"award-number":["P2022BCXTK","NUTRAGE FOE-2021 DBA.AD005.225"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Eotaxin-3 is a key chemokine with a relevant role in eosinophilic esophagitis, a rare chronic immune\/antigen-mediated inflammatory disorder. Eotaxin-3 is a potent activator of eosinophil emergence and migration, which may lead to allergic airway inflammation. We investigated, using bioinformatics tools, the protein structure and the possible effects of the known variations reported in public databases. Following a procedure already established, we created a 3D model of the whole protein and modeled the structure of 105 protein variants due to known point mutations. The effects of the amino acid substitution at the level of impact on protein structure, stability, and possibly function were detected by the bioinformatics procedure and described in detail. A web application was implemented to browse the results of the analysis and visualize the 3D models, with the opportunity of downloading the models and analyzing them using their own software. Among 105 amino acid substitutions investigated, the study evidenced in 44 cases at least one change in any of the investigated structural parameters. Other six variations are also relevant, although a structural effect was not detected by our analysis, because they affected amino acids highly conserved, which suggests a possible function role. All these variations should be the object of particular attention, as they may induce a loss of functionality in the protein.<\/jats:p>","DOI":"10.3390\/genes15081073","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T06:23:05Z","timestamp":1723616585000},"page":"1073","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioinformatics Study on Site-Specific Variations of Eotaxin-3, a Key Chemokine in Eosinophilic Esophagitis (EoE)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5838-1913","authenticated-orcid":false,"given":"Deborah","family":"Giordano","sequence":"first","affiliation":[{"name":"National Research Council, Institute of Food Science, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0516-0794","authenticated-orcid":false,"given":"Antonio","family":"d\u2019Acierno","sequence":"additional","affiliation":[{"name":"National Research Council, Institute of Food Science, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9510-5205","authenticated-orcid":false,"given":"Anna","family":"Marabotti","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology \u201cA. Zambelli\u201d, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9568-0680","authenticated-orcid":false,"given":"Paola","family":"Iovino","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Dentistry \u201cScuola Medica Salernitana\u201d, University of Salerno, 84081 Baronissi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5231-2231","authenticated-orcid":false,"given":"Giuseppe","family":"Iacomino","sequence":"additional","affiliation":[{"name":"National Research Council, Institute of Food Science, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7077-4912","authenticated-orcid":false,"given":"Angelo","family":"Facchiano","sequence":"additional","affiliation":[{"name":"National Research Council, Institute of Food Science, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1016\/j.cgh.2022.02.017","article-title":"International Consensus Recommendations for Eosinophilic Gastrointestinal Disease Nomenclature","volume":"20","author":"Dellon","year":"2022","journal-title":"Clin. 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