{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:10:03Z","timestamp":1785816603784,"version":"3.56.0"},"reference-count":72,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T00:00:00Z","timestamp":1735948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: Sperm samples are separated into bad and good quality samples in function of their phenotype, but this does not indicate their genetic quality. Methods: Here, we used GeneChip miRNA arrays to analyze microRNA expression in ten semen samples selected based on high-magnification morphology (score 6 vs. score 0) to identify miRNAs linked to sperm phenotype. Results: We found 86 upregulated and 21 downregulated miRNAs in good-quality sperm (score 6) compared with bad-quality sperm samples (score 0) (fold change &gt; 2 and p-value &lt; 0.05). MiR-34 (FC \u00d7 30, p = 8.43 \u00d7 10\u22128), miR-30 (FC \u00d7 12, p = 3.75 \u00d7 10\u22126), miR-122 (FC \u00d7 8, p = 0.0031), miR-20 (FC \u00d7 5.6, p = 0.0223), miR-182 (FC \u00d7 4.83, p = 0.0008) and miR-191 (FC \u00d7 4, p = 1.61 \u00d7 10\u22126) were among these upregulated miRNAs. In silico prediction algorithms predicted that miRNAs upregulated in good-quality sperm targeted 910 genes involved in key biological functions of spermatozoa, such as cell death and survival, cellular movement, molecular transport, response to stimuli, metabolism, and the regulation of oxidative stress. Genes deregulated in bad-quality sperm were involved in cell growth and proliferation. Conclusions: This study reveals that miRNA profiling may provide potential biomarkers of sperm quality.<\/jats:p>","DOI":"10.3390\/genes16010053","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T09:17:02Z","timestamp":1736155022000},"page":"53","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality"],"prefix":"10.3390","volume":"16","author":[{"given":"Nino-Guy","family":"Cassuto","sequence":"first","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5322-0141","authenticated-orcid":false,"given":"Florence","family":"Boitrelle","sequence":"additional","affiliation":[{"name":"Biology-Reproduction-Epigenetic-Environment-Development BREED, INRAE, Paris Saclay University, UVSQ, 78350 Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakima","family":"Mouik","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Pharmacy, University of Hassan II, Casablanca 28800, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Larue","sequence":"additional","affiliation":[{"name":"IVF ART Diaconesses Hospital, \u00eele de France, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwenola","family":"Keromnes","sequence":"additional","affiliation":[{"name":"IVF ART Diaconesses Hospital, \u00eele de France, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3166-2643","authenticated-orcid":false,"given":"Nathalie","family":"L\u00e9d\u00e9e","sequence":"additional","affiliation":[{"name":"IVF Center Bluets-Drouot, Les Bluets Hospital, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Part-Ellenberg","sequence":"additional","affiliation":[{"name":"IVF ART Bluets Hospital, \u00eele de France, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraldine","family":"Dray","sequence":"additional","affiliation":[{"name":"IVF ART Bluets Hospital, \u00eele de France, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a","family":"Ruoso","sequence":"additional","affiliation":[{"name":"ART Unit, Drouot Laboratory, 75009 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Rouen","sequence":"additional","affiliation":[{"name":"AP-HP, H\u00f4tel-Dieu, Sleep and Vigilance Center, Universit\u00e9 Paris Cit\u00e9, VIFASOM, ERC 7330, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1880-4130","authenticated-orcid":false,"given":"John","family":"De Vos","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine and Biotherapy (IRMB), University of Montpellier, INSERM, CHU Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6448-7995","authenticated-orcid":false,"given":"Said","family":"Assou","sequence":"additional","affiliation":[{"name":"Institute for Regenerative Medicine and Biotherapy (IRMB), University of Montpellier, INSERM, CHU Montpellier, 34295 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41572-023-00459-w","article-title":"Male Infertility","volume":"9","author":"Eisenberg","year":"2023","journal-title":"Nat. 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