{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:30:27Z","timestamp":1790998227890,"version":"4.1.0"},"reference-count":76,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T00:00:00Z","timestamp":1739577600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Mexicano del Seguro Social (IMSS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Colorectal cancer (CRC) is the second leading cause of cancer death worldwide. Variants in genes that regulate processes such as apoptosis and angiogenesis play a significant role in CRC. The objective of this study is to investigate the possible association between RASSF1 (rs2073498), SERPINE1 (rs1799889), EFNA1 (rs12904), and RAD51 (rs1801320) variants and clinicopathological characteristics of Mexican patients with CRC. Methods: DNA of peripheral blood samples was obtained from 631 individuals (349 patients and 282 control individuals). The RASSF1 (rs2073498), SERPINE1 (rs1799889), EFNA1 (rs12904), and RAD51 (rs1801320) variants were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The association was calculated using the odds ratio (OR) test. p-values were adjusted by the Bonferroni test (0.0125). In silico analysis programs, including Combined Annotation Dependent Depletion (CADD), Polymorphism Phenotyping-2 (PolyPhen-2), and Gene Expression Profiling Interactive Analysis (GEPIA), were conducted to predict the functional impact of these variants. Results: Patients carrying the G\/A genotype of the RASSF1 (rs2073498) variant showed an association with CRC characteristics, including TNM stages and tumor location (OR &gt; 2.5, p = 0.001). Regarding the SERPINE1 (rs1799889) variant, patients carrying the 5G\/4G genotype showed an association between TNM stages and tumor location in the rectum (OR &gt; 1.5, p \u2264 0.05). Patients with the G\/G genotype for the EFNA1 (rs12904) variant showed an association with TNM stages and rectal tumor location (OR &gt; 2.0, p = 0.001). The RAD51 (rs1801320) variant had no association with colorectal cancer. Conclusions: RASSF1 (rs2073498), SERPINE1 (rs1799889), and EFNA1 (rs12904) variants significantly influence colorectal cancer risk.<\/jats:p>","DOI":"10.3390\/genes16020223","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T04:40:25Z","timestamp":1739767225000},"page":"223","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic Variants in RASSF1 (rs2073498), SERPINE1 (rs1799889), and EFNA1 (rs12904) Are Associated with Susceptibility in Mexican Patients with Colorectal Cancer: Clinical Associations and Their Analysis In Silico"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2866-6633","authenticated-orcid":false,"given":"C\u00e9sar de Jes\u00fas","family":"Tovar-J\u00e1come","sequence":"first","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Ibet","family":"Ju\u00e1rez-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Direcci\u00f3n Acad\u00e9mica Aparatos y Sistemas I, Facultad de Medicina, Decanato Ciencias de la Salud, Universidad Aut\u00f3noma de Guadalajara (UAG), Zapopan 45129, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4539-1693","authenticated-orcid":false,"given":"Martha Patricia","family":"Gallegos-Arreola","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1504-1457","authenticated-orcid":false,"given":"Jos\u00e9 El\u00edas","family":"Garc\u00eda-Ortiz","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8352-236X","authenticated-orcid":false,"given":"Mar\u00eda Eugenia","family":"Mar\u00edn-Contreras","sequence":"additional","affiliation":[{"name":"Servicio de Gastroenterolog\u00eda, Hospital de Especialidades, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44329, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s Daniel","family":"Pineda-Razo","sequence":"additional","affiliation":[{"name":"Servicio de Oncolog\u00eda M\u00e9dica, Hospital de Especialidades, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44329, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Mariscal-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Servicio de Oncolog\u00eda M\u00e9dica, Hospital de Especialidades, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44329, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Dur\u00e1n-Anguiano","sequence":"additional","affiliation":[{"name":"Servicio de Coloproctolog\u00eda, Hospital de Especialidades, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44329, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo Antonio","family":"Alcaraz-Wong","sequence":"additional","affiliation":[{"name":"Servicio de Patolog\u00eda, Hospital de Especialidades, Centro M\u00e9dico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44329, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubria Alicia","family":"Gonz\u00e1lez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Lizbeth","family":"Mundaca-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam Yadira","family":"God\u00ednez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marl\u00edn","family":"Corona-Padilla","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica Alejandra","family":"Rosales-Reynoso","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Medicina Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. 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