{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:54:56Z","timestamp":1777388096566,"version":"3.51.4"},"reference-count":75,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovenian Research Agency","award":["P3-0124"],"award-info":[{"award-number":["P3-0124"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: Identifying the optimal bone regions for DNA analysis is critical, as DNA preservation and quality vary significantly across bone types and structures and is defined as intra-bone variability. This study aimed to evaluate the correlation between computed tomography (CT)-measured bone density and DNA preservation in small skeletal elements to identify optimal regions for DNA analysis. Methods: 137 bones from six skeletal elements excavated from a single burial site were analysed using Dual-Source CT (DSCT) to map compact and cancellous regions. DNA was extracted using a demineralisation method and quantified via real-time PCR to assess DNA quantity and degradation. Results: Among 461 bone regions analysed (137 bones; patellae, calcaneus, talus, the navicular bones, the cuboid bone, and the medial cuneiform bone), a significant difference in DNA quantity was observed only in the calcaneus, where the sulcus contained more DNA than the body. No significant differences in the degradation index were detected among bone segments or skeletal elements. Correlations between CT-measured bone density and DNA quantity or degradation index were region-specific. Higher bone density correlated positively with DNA quantity in compact regions of the calcaneus and talus. Regarding degradation, a positive correlation (higher bone density \u2192 higher degradation) was observed in the patella\u2019s anterior surface, while a negative correlation (higher bone density \u2192 lower degradation) was found in the talus\u2019s sulcus, the opposite side of the talar sulcus, the posterior calcaneal articular facet, and the cuboid\u2019s tuberosity. No significant correlations were found in other bone segments. Conclusions: Our study identified small skeletal elements, particularly the patella and the navicular bone, as promising sources for DNA analysis. While bone density correlated with DNA preservation in some cases, the relationship was inconsistent. Our findings support the use of small bones in forensic and archaeological research and warrant further investigation.<\/jats:p>","DOI":"10.3390\/genes16030291","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T04:28:18Z","timestamp":1740716898000},"page":"291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Targeting Optimal Bone Regions: Correlations Between Bone Density and DNA Quality in Small Skeletal Elements"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2335-2263","authenticated-orcid":false,"given":"\u017diva Miriam","family":"Ger\u0161ak","sequence":"first","affiliation":[{"name":"Clinical Institute of Radiology, University Medical Centre Ljubljana, Zalo\u0161ka 7, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladka","family":"Salapura","sequence":"additional","affiliation":[{"name":"Clinical Institute of Radiology, University Medical Centre Ljubljana, Zalo\u0161ka 7, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7449-7038","authenticated-orcid":false,"given":"Eva","family":"Podov\u0161ovnik","sequence":"additional","affiliation":[{"name":"Faculty of Tourism Studies-Turistica, University of Primorska, Obala 11a, 6320 Portoro\u017e, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-015X","authenticated-orcid":false,"given":"Irena","family":"Zupani\u010d-Pajni\u010d","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Emmons, A.L., Mundorff, A.Z., Hoeland, K.M., Davoren, J., Keenan, S.W., Carter, D.O., Campagna, S.R., and DeBruyn, J.M. (2022). Postmortem Skeletal Microbial Community Composition and Function in Buried Human Remains. mSystems, 7.","DOI":"10.1128\/msystems.00041-22"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102818","DOI":"10.1016\/j.fsigen.2022.102818","article-title":"Forensic DNA Extraction Methods for Human Hard Tissue: A Systematic Literature Review and Meta-Analysis of Technologies and Sample Type","volume":"63","author":"Finaughty","year":"2023","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102272","DOI":"10.1016\/j.fsigen.2020.102272","article-title":"Reconstructing Full and Partial STR Profiles from Severely Burned Human Remains Using Comparative Ancient and Forensic DNA Extraction Techniques","volume":"46","author":"Emery","year":"2020","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4724","DOI":"10.1098\/rspb.2012.1745","article-title":"The Half-Life of DNA in Bone: Measuring Decay Kinetics in 158 Dated Fossils","volume":"279","author":"Allentoft","year":"2012","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1038\/nprot.2007.247","article-title":"Ancient DNA Extraction from Bones and Teeth","volume":"2","author":"Rohland","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110859","DOI":"10.1016\/j.forsciint.2021.110859","article-title":"Intrinsic and Extrinsic Factors That May Influence DNA Preservation in Skeletal Remains: A Review","volume":"325","author":"Raffone","year":"2021","journal-title":"Forensic Sci. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"102196","DOI":"10.1016\/j.fsigen.2019.102196","article-title":"ATR-FTIR Spectroscopy Combined with Data Manipulation as a Pre-Screening Method to Assess DNA Preservation in Skeletal Remains","volume":"44","author":"Leskovar","year":"2020","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_8","unstructured":"Boddington, A., Garland, A.N., and Janaway, R.C. (1987). The Relative Survival of the Human Skeleton: Implications for Palaeopathology. Death, Decay, and Reconstruction: Approaches to Archaeology and Forensic Science, Manchester University Press."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0305-4403(84)90005-0","article-title":"Experimental Effects of Bone Size and Temperature on Bone Diagenesis","volume":"11","author":"Ortner","year":"1984","journal-title":"J. Archaeol. Sci."},{"key":"ref_10","unstructured":"Cox, M., and Mays, S. (2000). The Chemical Degradation of Bones. Human Osteology in Archaeology and Forensic Science, Greenwich Medical Media."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1073\/pnas.86.6.1939","article-title":"Ancient DNA: Extraction, Characterization, Molecular Cloning, and Enzymatic Amplification","volume":"86","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","unstructured":"William, G. (2016). Extraction of DNA from Human Skeletal Material. Forensic DNA Typing Protocols, Humana Press."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.fsigen.2007.02.006","article-title":"High Efficiency DNA Extraction from Bone by Total Demineralization","volume":"1","author":"Loreille","year":"2007","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.fsigen.2011.08.004","article-title":"Automatable Full Demineralization DNA Extraction Procedure from Degraded Skeletal Remains","volume":"6","author":"Amory","year":"2012","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1002\/ajpa.21193","article-title":"Technical Note: Efficiency of Total Demineralization and Ion-exchange Column for DNA Extraction from Bone","volume":"141","author":"Seo","year":"2010","journal-title":"Am. J. Phys. Anthropol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.fsigen.2013.05.002","article-title":"A DNA Extraction Method of Small Quantities of Bone for High-Quality Genotyping","volume":"7","author":"Caputo","year":"2013","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102882","DOI":"10.1016\/j.fsigen.2023.102882","article-title":"A Fast and Highly Efficient Automated DNA Extraction Method from Small Quantities of Bone Powder from Aged Bone Samples","volume":"65","author":"Leskovar","year":"2023","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"102305","DOI":"10.1016\/j.fsigen.2020.102305","article-title":"The Petrous Bone: Ideal Substrate in Legal Medicine?","volume":"47","author":"Gonzalez","year":"2020","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hansen, H.B., Damgaard, P.B., Margaryan, A., Stenderup, J., Lynnerup, N., Willerslev, E., and Allentoft, M.E. (2017). Comparing Ancient DNA Preservation in Petrous Bone and Tooth Cementum. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0170940"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.forsciint.2017.12.023","article-title":"Neither Femur nor Tooth: Petrous Bone for Identifying Archaeological Bone Samples via Forensic Approach","volume":"283","author":"Pilli","year":"2018","journal-title":"Forensic Sci. Int."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pinhasi, R., Fernandes, D., Sirak, K., Novak, M., Connell, S., Alpaslan-Roodenberg, S., Gerritsen, F., Moiseyev, V., Gromov, A., and Raczky, P. (2015). Optimal Ancient DNA Yields from the Inner Ear Part of the Human Petrous Bone. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0129102"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"102578","DOI":"10.1016\/j.fsigen.2021.102578","article-title":"Comparison of Nuclear DNA Yield and STR Typing Success in Second World War Petrous Bones and Metacarpals III","volume":"55","author":"Inkret","year":"2021","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Ibrahim, J., Brumfeld, V., Addadi, Y., Rubin, S., Weiner, S., and Boaretto, E. (2022). The Petrous Bone Contains High Concentrations of Osteocytes: One Possible Reason Why Ancient DNA Is Better Preserved in This Bone. PLoS ONE, 17.","DOI":"10.1101\/2022.05.20.492830"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1127\/anthranz\/2020\/1263","article-title":"Morphometric Analysis of the Petrous Bone\u2014A Preliminary Study of Intra- and Inter-Observer Variation of Basic Measurements","volume":"78","author":"Krause","year":"2021","journal-title":"Anthropol. Anz."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1007\/s00414-021-02528-9","article-title":"Intra-Bone Nuclear DNA Variability in Second World War Metatarsal and Metacarpal Bones","volume":"135","author":"Inkret","year":"2021","journal-title":"Int. J. Leg. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1007\/s00414-022-02860-8","article-title":"Comparison of DNA Preservation between Ribs and Vertebrae","volume":"136","author":"Zupanc","year":"2022","journal-title":"Int. J. Leg. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"102587","DOI":"10.1016\/j.fsigen.2021.102587","article-title":"Intra-Bone Nuclear DNA Variability and STR Typing Success in Second World War 12th Thoracic Vertebrae","volume":"55","author":"Zupanc","year":"2021","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1007\/s00414-021-02681-1","article-title":"Intra-Bone Nuclear DNA Variability and STR Typing Success in Second World War First Ribs","volume":"135","author":"Zupanc","year":"2021","journal-title":"Int. J. Leg. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103067","DOI":"10.1016\/j.fsigen.2024.103067","article-title":"DNA Preservation in Compact and Trabecular Bone","volume":"71","year":"2024","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_30","first-page":"473","article-title":"Patellae as a Source of DNA in Forensic and Archaeological Analysis","volume":"139","author":"Golob","year":"2024","journal-title":"Int. J. Leg. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.fsigen.2013.08.001","article-title":"Examination of DNA Yield Rates for Different Skeletal Elements at Increasing Post Mortem Intervals","volume":"8","author":"Mundorff","year":"2014","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102193","DOI":"10.1016\/j.fsigen.2019.102193","article-title":"Inter and Intra-Individual Variation in Skeletal DNA Preservation in Buried Remains","volume":"44","author":"Emmons","year":"2020","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/1556-4029.13843","article-title":"Intra- and Inter-Element Variability in Mitochondrial and Nuclear <scp>DNA<\/Scp> from Fresh and Environmentally Exposed Skeletal Remains","volume":"64","author":"Antinick","year":"2019","journal-title":"J. Forensic Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1007\/s00414-022-02881-3","article-title":"Comparison of DNA Preservation between Adult and Non-Adult Ancient Skeletons","volume":"136","author":"Mesesnel","year":"2022","journal-title":"Int. J. Leg. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.fsigen.2017.03.002","article-title":"Evaluating Differential Nuclear DNA Yield Rates and Osteocyte Numbers among Human Bone Tissue Types: A Synchrotron Radiation Micro-CT Approach","volume":"28","author":"Andronowski","year":"2017","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1111\/1755-0998.12911","article-title":"Optimized DNA Sampling of Ancient Bones Using Computed Tomography Scans","volume":"18","author":"Alberti","year":"2018","journal-title":"Mol. Ecol. Resour."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1148\/radiol.2015142631","article-title":"Dual- and Multi-Energy CT: Principles, Technical Approaches, and Clinical Applications","volume":"276","author":"McCollough","year":"2015","journal-title":"Radiology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"493","DOI":"10.2214\/AJR.19.21095","article-title":"Dual-Energy CT in Musculoskeletal Imaging: What Is the Role Beyond Gout?","volume":"213","author":"Rajiah","year":"2019","journal-title":"Am. J. Roentgenol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Choi, K.Y., Lee, S.-W., In, Y., Kim, M.S., Kim, Y.D., Lee, S., Lee, J.-W., and Koh, I.J. (2022). Dual-Energy CT-Based Bone Mineral Density Has Practical Value for Osteoporosis Screening around the Knee. Medicina, 58.","DOI":"10.3390\/medicina58081085"},{"key":"ref_40","unstructured":"Klein Jeffrey, S., Brant William, E., Helms Clyde, A., and Vinson Emily, N. (2019). Diagnostic Imaging Methods. Brant and Helm\u2019s Fundamentals of Diagnostic Radiology, Wolters Kluwer."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/362709a0","article-title":"Instability and Decay of the Primary Structure of DNA","volume":"362","author":"Lindahl","year":"1993","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1093\/nar\/24.7.1304","article-title":"DNA Damage and DNA Sequence Retrieval from Ancient Tissues","volume":"24","author":"Hoss","year":"1996","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1126\/science.272.5263.864","article-title":"Amino Acid Racemization and the Preservation of Ancient DNA","volume":"272","author":"Poinar","year":"1996","journal-title":"Science"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1007\/BF01921721","article-title":"The Biochemistry of Ancient DNA in Bone","volume":"50","author":"Tuross","year":"1994","journal-title":"Experientia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0047-2484(03)00106-4","article-title":"The Thermal History of Human Fossils and the Likelihood of Successful DNA Amplification","volume":"45","author":"Smith","year":"2003","journal-title":"J. Hum. Evol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1073\/pnas.0610257104","article-title":"Freshly Excavated Fossil Bones Are Best for Amplification of Ancient DNA","volume":"104","author":"Pruvost","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","unstructured":"Miriam, G.\u017d., Irena, Z.-P., Eva, P., and Vladka, S. (2025). Computed Tomography Differentiation of Compact and Cancellous Bone Tissue in Short and Sesamoid Bones. Radiol. Oncol., 59, in press."},{"key":"ref_48","unstructured":"Promega Corporation (2022). PowerQuant System Technical Manual, Promega Corporation."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"110994","DOI":"10.1016\/j.forsciint.2021.110994","article-title":"Identification of a Slovenian Prewar Elite Couple Killed in the Second World War","volume":"327","year":"2021","journal-title":"Forensic Sci. Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s00414-023-03003-3","article-title":"Improving Kinship Probability in Analysis of Ancient Skeletons Using Identity SNPs and MPS Technology","volume":"137","author":"Leskovar","year":"2023","journal-title":"Int. J. Leg. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1214\/ss\/1032280214","article-title":"Bootstrap Confidence Intervals","volume":"11","author":"DiCiccio","year":"1996","journal-title":"Stat. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1002\/(SICI)1097-0258(20000515)19:9<1141::AID-SIM479>3.0.CO;2-F","article-title":"Bootstrap Confidence Intervals: When, Which, What? A Practical Guide for Medical Statisticians","volume":"19","author":"Carpenter","year":"2000","journal-title":"Stat. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.fsigss.2009.09.029","article-title":"Sampling of the Cranium for Mitochondrial DNA Analysis of Human Skeletal Remains","volume":"2","author":"Edson","year":"2009","journal-title":"Forensic Sci. Int. Genet. Suppl. Ser."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1038\/ncomms6257","article-title":"Genome Flux and Stasis in a Five Millennium Transect of European Prehistory","volume":"5","author":"Gamba","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00414-017-1653-z","article-title":"As Solid as a Rock\u2014Comparison of CE- and MPS-Based Analyses of the Petrosal Bone as a Source of DNA for Forensic Identification of Challenging Cranial Bones","volume":"132","author":"Kulstein","year":"2018","journal-title":"Int. J. Leg. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Parker, C., Rohrlach, A.B., Friederich, S., Nagel, S., Meyer, M., Krause, J., Bos, K.I., and Haak, W. (2020). A Systematic Investigation of Human DNA Preservation in Medieval Skeletons. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-75163-w"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.ajhg.2017.06.013","article-title":"Continuity and Admixture in the Last Five Millennia of Levantine History from Ancient Canaanite and Present-Day Lebanese Genome Sequences","volume":"101","author":"Haber","year":"2017","journal-title":"Am. J. Human. Genet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1111\/1755-0998.12931","article-title":"Ancient DNA: The Quest for the Best","volume":"18","author":"Geigl","year":"2018","journal-title":"Mol. Ecol. Resour."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Furtw\u00e4ngler, A., Reiter, E., Neumann, G.U., Siebke, I., Steuri, N., Hafner, A., L\u00f6sch, S., Anthes, N., Schuenemann, V.J., and Krause, J. (2018). Ratio of Mitochondrial to Nuclear DNA Affects Contamination Estimates in Ancient DNA Analysis. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-32083-0"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.aanat.2011.07.003","article-title":"DNA in Ancient Bone\u2014Where Is It Located and How Should We Extract It?","volume":"194","author":"Campos","year":"2012","journal-title":"Ann. Anat.\u2014Anat. Anz."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1101\/gr.260141.119","article-title":"Human Auditory Ossicles as an Alternative Optimal Source of Ancient DNA","volume":"30","author":"Sirak","year":"2020","journal-title":"Genome Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"112184","DOI":"10.1016\/j.forsciint.2024.112184","article-title":"Searching for Alternative High DNA-Yielding Bone Types for DNA Analysis of Aged Skeletal Remains","volume":"362","author":"Golob","year":"2024","journal-title":"Forensic Sci. Int."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Gaudio, D., Fernandes, D.M., Schmidt, R., Cheronet, O., Mazzarelli, D., Mattia, M., O\u2019Keeffe, T., Feeney, R.N.M., Cattaneo, C., and Pinhasi, R. (2019). Genome-Wide DNA from Degraded Petrous Bones and the Assessment of Sex and Probable Geographic Origins of Forensic Cases. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-44638-w"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.fsigen.2010.08.008","article-title":"Reliable Genetic Identification of Burnt Human Remains","volume":"5","author":"Schwark","year":"2011","journal-title":"Forensic Sci. Int. Genet."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.forsciint.2015.08.019","article-title":"The Effect of Common Imaging and Hot Water Maceration on DNA Recovery from Skeletal Remains","volume":"257","author":"Frank","year":"2015","journal-title":"Forensic Sci. Int."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.1016\/j.jas.2010.07.013","article-title":"Chemical, Elemental, and Isotopic Effects of Acid Concentration and Treatment Duration on Ancient Bone Collagen: An Exploratory Study","volume":"37","author":"Pestle","year":"2010","journal-title":"J. Archaeol. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Turner-Walker, G. (2007). The Chemical and Microbial Degradation of Bones and Teeth. Advances in Human Palaeopathology, Wiley.","DOI":"10.1002\/9780470724187.ch1"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jas.2003.07.007","article-title":"Characterisation of Microbial Attack on Archaeological Bone","volume":"31","author":"Jans","year":"2004","journal-title":"J. Archaeol. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e22246","DOI":"10.1097\/MD.0000000000022246","article-title":"Microstructure of the Hyoid Bone Based on Micro-Computed Tomography Findings","volume":"99","author":"Wang","year":"2020","journal-title":"Medicine"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1127\/homo\/2020\/1133","article-title":"Micro-CT Analysis of the Internal Acoustic Meatus Angles as a Method of Sex Estimation in Skeletal Remains","volume":"71","author":"Kozerska","year":"2020","journal-title":"HOMO"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"105646","DOI":"10.1016\/j.jas.2022.105646","article-title":"3D Visualization of Bioerosion in Archaeological Bone","volume":"145","author":"Mandl","year":"2022","journal-title":"J. Archaeol. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"151675","DOI":"10.1016\/j.aanat.2021.151675","article-title":"Dental Pulp Calcifications in Prehistoric and Historical Skeletal Remains","volume":"235","author":"Nicklisch","year":"2021","journal-title":"Ann. Anat.\u2014Anat. Anz."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"101914","DOI":"10.1016\/j.legalmed.2021.101914","article-title":"Evaluation of the Detectability of Early Mandible Fracture Healing Findings in Terms of Vitality Aspect by Using Micro-CT Technology in Postmortem Interval","volume":"52","author":"Akbulut","year":"2021","journal-title":"Leg. Med."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Viero, A., Biehler-Gomez, L., Messina, C., Cappella, A., Giannoukos, K., Viel, G., Tagliaro, F., and Cattaneo, C. (2022). Utility of Micro-CT for Dating Post-Cranial Fractures of Known Post-Traumatic Ages through 3D Measurements of the Trabecular Inner Morphology. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-14530-1"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Thurzo, A., Jan\u010dovi\u010dov\u00e1, V., Hain, M., Thurzo, M., Nov\u00e1k, B., Kosn\u00e1\u010dov\u00e1, H., Lehotsk\u00e1, V., Varga, I., Kov\u00e1\u010d, P., and Moravansk\u00fd, N. (2022). Human Remains Identification Using Micro-CT, Chemometric and AI Methods in Forensic Experimental Reconstruction of Dental Patterns after Concentrated Sulphuric Acid Significant Impact. 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