{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:22:00Z","timestamp":1785813720815,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["[2022]099"],"award-info":[{"award-number":["[2022]099"]}]},{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["[2022]097"],"award-info":[{"award-number":["[2022]097"]}]},{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["[2023]004"],"award-info":[{"award-number":["[2023]004"]}]},{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["2024YFD2000300"],"award-info":[{"award-number":["2024YFD2000300"]}]},{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["2024YFF1001400"],"award-info":[{"award-number":["2024YFF1001400"]}]},{"name":"Guizhou Provincial Key Technology R&amp;D Program","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"name":"National Key R &amp; D Program of China","award":["[2022]099"],"award-info":[{"award-number":["[2022]099"]}]},{"name":"National Key R &amp; D Program of China","award":["[2022]097"],"award-info":[{"award-number":["[2022]097"]}]},{"name":"National Key R &amp; D Program of China","award":["[2023]004"],"award-info":[{"award-number":["[2023]004"]}]},{"name":"National Key R &amp; D Program of China","award":["2024YFD2000300"],"award-info":[{"award-number":["2024YFD2000300"]}]},{"name":"National Key R &amp; D Program of China","award":["2024YFF1001400"],"award-info":[{"award-number":["2024YFF1001400"]}]},{"name":"National Key R &amp; D Program of China","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]},{"name":"China Agriculture Research System","award":["[2022]099"],"award-info":[{"award-number":["[2022]099"]}]},{"name":"China Agriculture Research System","award":["[2022]097"],"award-info":[{"award-number":["[2022]097"]}]},{"name":"China Agriculture Research System","award":["[2023]004"],"award-info":[{"award-number":["[2023]004"]}]},{"name":"China Agriculture Research System","award":["2024YFD2000300"],"award-info":[{"award-number":["2024YFD2000300"]}]},{"name":"China Agriculture Research System","award":["2024YFF1001400"],"award-info":[{"award-number":["2024YFF1001400"]}]},{"name":"China Agriculture Research System","award":["CARS-40"],"award-info":[{"award-number":["CARS-40"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Eggshell quality is a critical factor influencing consumer preference and the economic benefits of poultry enterprises, and the uterus is the key site for eggshell synthesis. Yaoshan chicken (YS), an indigenous chicken breed in China, is renowned for its flavorful meat and high-quality eggs. However, its egg production is lower compared to specialized strains. Therefore, the GYR crossbreed was developed by three-line hybridization for YS chicken, which can produce green-shelled eggs with better eggshell thickness and strength than YS chicken (p &lt; 0.01). To explore the molecular mechanisms underlying the differences in eggshell quality between GYR and YS chickens, we conducted an integrated transcriptomic and metabolomic analysis. Methods: Twelve uterus samples (six from GYR and six from YS chickens) were collected during the period of eggshell calcification at 260 days of age. RNA sequencing (RNA-seq) and liquid chromatography\u2013mass spectrometry (LC-MS\/MS) were performed to identify differentially expressed genes (DEGs) and differential metabolites (DMs), respectively. Results: A total of 877 DEGs were identified in the GYR group, including 196 upregulated and 681 downregulated genes (|log2 (fold change)| &gt; 1, p-value &lt; 0.05). Additionally, 79 DMs were detected, comprising 50 upregulated and 29 downregulated metabolites (|log\u2082 (fold change)| &gt; 1, VIP &gt; 1). Notably, the key DEGs (SLCO1B3, SLCO1B1, PTGR1, LGR6, MELTF, CRISP2, GVINP1, and OVSTL), important DMs (prostaglandin-related DMs and biliverdin) and signaling pathways (calcium signaling, neuroactive ligand\u2013receptor interaction, arachidonic acid metabolism, bile secretion, and primary bile acid biosynthesis) were major regulators of the eggshell quality. Furthermore, an integrated transcriptomic and metabolomic analysis revealed two significant gene\u2013metabolite pairs associated with eggshell quality: PTGDS\u2013prostaglandin E2 and PTGS1\u2013prostaglandin E2. Conclusions: This study provides a theoretical foundation for the improved eggshell quality of Yaoshan chicken.<\/jats:p>","DOI":"10.3390\/genes16040383","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T04:36:48Z","timestamp":1743136608000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptomic and Metabolomic Analysis of the Uterine Tissue of Yaoshan Chicken and Its Crossbreeds to Reveal the Molecular Mechanism Influencing Eggshell Quality"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9551-7536","authenticated-orcid":false,"given":"Xiaomeng","family":"Miao","sequence":"first","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"},{"name":"Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Guizhou Province Livestock and Poultry Genetic Resources Management Station, Guizhou Provincial Department of Agriculture and Rural Affairs, Guiyang 550001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Gong","sequence":"additional","affiliation":[{"name":"Guizhou Province Livestock and Poultry Genetic Resources Management Station, Guizhou Provincial Department of Agriculture and Rural Affairs, Guiyang 550001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fugui","family":"Li","sequence":"additional","affiliation":[{"name":"Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science and Technology, Tarim University, Alar 843300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyue","family":"Liang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diyan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Chengdu University, Chengdu 610106, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Ning","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.psj.2020.10.001","article-title":"Dietary green tea powder supplementation enriched egg nutrients and physicochemical property in an indigenous chicken breed","volume":"100","author":"Chen","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3382\/ps\/pew289","article-title":"Effect of production system and flock age on eggshell and egg internal quality measurements","volume":"96","author":"Samiullah","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1080\/00071668.2020.1758299","article-title":"Endocrine and genetic factors affecting egg laying performance in chickens: A review","volume":"61","author":"Du","year":"2020","journal-title":"Br. Poult. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1080\/00071668.2020.1792834","article-title":"Identification of differentially expressed genes and signalling pathways in the ovary of higher and lower laying ducks","volume":"61","author":"Sun","year":"2020","journal-title":"Br. Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4378","DOI":"10.3382\/ps\/pex229","article-title":"Comparative transcriptomic analysis of high and low egg-producing duck ovaries","volume":"96","author":"Tao","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1002\/vms3.575","article-title":"Comparative transcriptomic analysis of ovaries from high and low egg-laying Lingyun black-bone chickens","volume":"7","author":"Zhang","year":"2021","journal-title":"Vet. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"106114","DOI":"10.1016\/j.anireprosci.2019.106114","article-title":"Transcriptome analysis of ovary in relatively greater and lesser egg producing Jinghai Yellow Chicken","volume":"208","author":"Zhang","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1080\/00071668.2019.1621992","article-title":"Physical and mechanical properties of eggshell as affected by chicken breed and flock age","volume":"60","author":"Fathi","year":"2019","journal-title":"Br. Poult. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101273","DOI":"10.1016\/j.psj.2021.101273","article-title":"Mechanisms associated with the depigmentation of brown eggshells: A review","volume":"100","author":"Lu","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_10","first-page":"235","article-title":"Analysis and evaluation of muscle nutrient composition in Yaoshan chicken and Changshun green-shelled egg-laying chicken","volume":"57","author":"Tang","year":"2021","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_11","first-page":"1923","article-title":"TGF\u03b23 gene polymorphism and correlations between TGF\u03b23 gene and reproduction traits in Liboyaoshan chicken","volume":"30","author":"Wu","year":"2017","journal-title":"Southwest. China J. Agric. Sci."},{"key":"ref_12","unstructured":"Guizhou Institute of Animal Husbandry and Veterinary Medicine (2023). Preliminary breeding success of new high-quality local chicken lines in Guizhou. Guizhou J. Anim. Husb. Vet., 47, 5. (In Chinese)."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Miao, X., Wu, T., Pan, H., Zhang, Y., Liu, J., Fan, Y., Du, L., Gong, Y., Li, L., and Huang, T. (2024). Integrative analysis of the ovarian metabolome and transcriptome of the Yaoshan chicken and its improved hybrids. Front. Genet., 15.","DOI":"10.3389\/fgene.2024.1416283"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Khan, S., Wu, S.B., and Roberts, J. (2019). RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens. BMC Genom., 20.","DOI":"10.1186\/s12864-019-5460-4"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Brionne, A., Nys, Y., Hennequet-Antier, C., and Gautron, J. (2014). Hen uterine gene expression profiling during eggshell formation reveals putative proteins involved in the supply of minerals or in the shell mineralization process. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-220"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100970","DOI":"10.1016\/j.psj.2020.12.067","article-title":"Effects of dietary Bacillus subtilis supplementation and calcium levels on performance and eggshell quality of laying hens in the late phase of production","volume":"100","author":"Wang","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.3382\/ps.0701236","article-title":"Whitening of brown-shelled eggs: Mineral composition of uterine fluid and rate of protoporphyrin deposition","volume":"70","author":"Nys","year":"1991","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1111\/asj.12182","article-title":"Quantitative expression of candidate genes affecting eggshell color","volume":"85","author":"Zheng","year":"2014","journal-title":"Anim. Sci. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1093\/ps\/85.3.546","article-title":"A study on eggshell pigmentation: Biliverdin in blue-shelled chickens","volume":"85","author":"Zhao","year":"2006","journal-title":"Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"giaf006","DOI":"10.1093\/gigascience\/giaf006","article-title":"Multiomics analysis provides insights into musk secretion in muskrat and musk deer","volume":"14","author":"Wang","year":"2025","journal-title":"Gigascience"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pan, H., Huang, T., Yu, L., Wang, P., Su, S., Wu, T., Bai, Y., Teng, Y., Wei, Y., and Zhou, L. (2022). Transcriptome Analysis of the Adipose Tissue of Luchuan and Duroc Pigs. Animals, 12.","DOI":"10.3390\/ani12172258"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1016\/j.jgg.2024.08.002","article-title":"Insights into left-right asymmetric development of chicken ovary at the single-cell level","volume":"51","author":"Wang","year":"2024","journal-title":"J. Genet. Genom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.24272\/j.issn.2095-8137.2024.037","article-title":"Single nucleus\/cell RNA-seq of the chicken hypothalamic-pituitary-ovarian axis offers new insights into the molecular regulatory mechanisms of ovarian development","volume":"45","author":"Leng","year":"2024","journal-title":"Zool. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1080\/00071668.2020.1849556","article-title":"Metabolomic evaluation of serum metabolites of geese reared at different stocking densities","volume":"62","author":"Ying","year":"2021","journal-title":"Br. Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"108642","DOI":"10.1016\/j.meatsci.2021.108642","article-title":"Integrative analysis of transcriptomic and metabolomic profiles reveal the complex molecular regulatory network of meat quality in Enshi black pigs","volume":"183","author":"Zhan","year":"2022","journal-title":"Meat. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e185","DOI":"10.1002\/imt2.185","article-title":"Vaginal microbiota are associated with in vitro fertilization during female infertility","volume":"3","author":"Wang","year":"2024","journal-title":"Imeta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104181","DOI":"10.1016\/j.psj.2024.104181","article-title":"Research Note: Correlation between the reproductive tract microbiota and speckled eggs in laying hens","volume":"103","author":"Cheng","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"106826","DOI":"10.1016\/j.anireprosci.2021.106826","article-title":"Uterine structure and function contributes to the formation of the sandpaper-shelled eggs in laying hens","volume":"232","author":"Khogali","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Sah, N., Kuehu, D.L., Khadka, V.S., Deng, Y., Peplowska, K., Jha, R., and Mishra, B. (2018). RNA sequencing-based analysis of the laying hen uterus revealed the novel genes and biological pathways involved in the eggshell biomineralization. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-35203-y"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"609","DOI":"10.5713\/ajas.2012.12398","article-title":"Microarray analysis of genes involved with shell strength in layer shell gland at the early stage of active calcification","volume":"26","author":"Liu","year":"2013","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Chen, S., Chen, K., Xu, J., Li, F., Ding, J., Ma, Z., Li, G., and Li, H. (2021). Insights Into mRNA and Long Non-coding RNA Profiling RNA Sequencing in Uterus of Chickens With Pink and Blue Eggshell Colors. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.736387"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, Z., Meng, G., Bai, Y., Liu, R., Du, Y., and Su, L. (2017). Comparative transcriptome analysis provides clues to molecular mechanisms underlying blue-green eggshell color in the Jinding duck (Anas platyrhynchos). BMC Genom., 18.","DOI":"10.1186\/s12864-017-4135-2"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1071\/AN12345","article-title":"Egg quality and age of laying hens: Implications for product safety","volume":"53","author":"Roberts","year":"2013","journal-title":"Anim. Prod. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zi, X., Ge, X., Zhu, Y., Liu, Y., Sun, D., Li, Z., Liu, M., You, Z., Wang, B., and Kang, J. (2023). Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens. Vet. Sci., 10.","DOI":"10.3390\/vetsci10050341"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"110485","DOI":"10.1016\/j.ygeno.2022.110485","article-title":"Genomic and transcriptomic analyses reveal genetic adaptation to cold conditions in the chickens","volume":"114","author":"Zhao","year":"2022","journal-title":"Genomics"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ouyang, Q., Hu, S., Tang, B., Hu, B., Hu, J., He, H., Li, L., and Wang, J. (2022). Comparative Transcriptome Analysis Provides Novel Insights into the Effect of Lipid Metabolism on Laying of Geese. Animals, 12.","DOI":"10.3390\/ani12141775"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Xiang, X., Huang, X., Wang, J., Zhang, H., Zhou, W., Xu, C., Huang, Y., Tan, Y., and Yin, Z. (2023). Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period. Front. Genet., 14.","DOI":"10.3389\/fgene.2023.1222087"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1080\/19768354.2022.2146187","article-title":"Blood transcriptome comparison between sexes and their function in 4-week Rhode Island red chickens","volume":"26","author":"Kim","year":"2022","journal-title":"Anim. Cells. Syst"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Sun, T., Xiao, C., Yang, Z., Deng, J., and Yang, X. (2022). Grade follicles transcriptional profiling analysis in different laying stages in chicken. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08728-w"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Chen, X., Cao, J., Chang, C., Geng, A., Wang, H., Chu, Q., Yan, Z., Zhang, X., Zhang, Y., and Liu, H. (2023). Effects of Age on Compounds, Metabolites and Meat Quality in Beijing-You Chicken Breast Meat. Animals, 13.","DOI":"10.3390\/ani13213419"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"103118","DOI":"10.1016\/j.psj.2023.103118","article-title":"Integrating proteomics and metabolomics to elucidate the molecular network regulating of inosine monophosphate-specific deposition in Jingyuan chicken","volume":"102","author":"Huang","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"101604","DOI":"10.1016\/j.psj.2021.101604","article-title":"Untargeted metabolomics study on the effects of rearing ducks in cages on bone quality","volume":"101","author":"Yang","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wu, Y., Xiao, H., Zhang, H., Pan, A., Shen, J., Sun, J., Liang, Z., and Pi, J. (2023). Quasi-Targeted Metabolomics Approach Reveal the Metabolite Differences of Three Poultry Eggs. Foods, 12.","DOI":"10.3390\/foods12142765"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"9060","DOI":"10.3390\/cimb45110568","article-title":"Multi-Omics Analyses Unravel Metabolic and Transcriptional Differences in Tender Shoots from Two Sechium edule Varieties","volume":"45","author":"Du","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"103220","DOI":"10.1016\/j.psj.2023.103220","article-title":"Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities","volume":"103","author":"Xu","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3747","DOI":"10.3382\/ps\/pex187","article-title":"Recent advances in avian egg science: A review","volume":"96","author":"Wilson","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"832","DOI":"10.14202\/vetworld.2016.832-836","article-title":"Body weight, egg production, and egg quality traits of gray, brown, and white varieties of Japanese quail (Coturnix coturnix japonica) in coastal climatic condition of Odisha","volume":"9","author":"Bagh","year":"2016","journal-title":"Vet. World"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1016\/j.psj.2019.11.023","article-title":"A comparison between the quality of eggs from indigenous chicken breeds and that from commercial layers","volume":"99","author":"Lordelo","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Sun, T., Xiao, C., Yang, Z., Deng, J., and Yang, X. (2023). Transcriptome profiling analysis of uterus during chicken laying periods. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09521-z"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"103212","DOI":"10.1016\/j.psj.2023.103212","article-title":"Refine localizations of functional variants affecting eggshell color of Lueyang black-boned chicken in the SLCO1B3","volume":"103","author":"Wang","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1080\/00498250801986951","article-title":"Xenobiotic transporters of the human organic anion transporting polypeptides (OATP) family","volume":"38","author":"Hagenbuch","year":"2008","journal-title":"Xenobiotica"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.rvsc.2019.03.004","article-title":"The expression of prostaglandins-related genes in erythrocytes of broiler chicken responds to thiram-induced tibial dyschondroplasia and recombinant glutathione-S-transferase A3 protein","volume":"124","author":"Niu","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"117207","DOI":"10.1016\/j.bone.2024.117207","article-title":"Signaling pathways associated with Lgr6 to regulate osteogenesis","volume":"187","author":"King","year":"2024","journal-title":"Bone"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1007\/s43032-022-01101-y","article-title":"MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration","volume":"30","author":"Mao","year":"2023","journal-title":"Reprod. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1210\/en.2018-01076","article-title":"CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility","volume":"160","author":"Lim","year":"2019","journal-title":"Endocrinology"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wang, Y., Zhu, M., Guo, F., Song, Y., Fan, X., and Qin, G. (2020). Identification of Tumor Microenvironment-Related Prognostic Biomarkers in Luminal Breast Cancer. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.555865"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"10448","DOI":"10.1021\/acs.jafc.3c00708","article-title":"Comparative N-Glycoproteomic Investigation of Eggshell Cuticle and Mineralized Layer Proteins","volume":"71","author":"Zeng","year":"2023","journal-title":"J. Agric. Food. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"485","DOI":"10.3382\/ps.2013-03557","article-title":"RNA-seq analysis revealed novel genes and signaling pathway associated with disease resistance to avian influenza virus infection in chickens","volume":"93","author":"Wang","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Zhang, Q., Zhu, F., Liu, L., Zheng, C.W., Wang, D.H., Hou, Z.C., and Ning, Z.H. (2015). Integrating transcriptome and genome re-sequencing data to identify key genes and mutations affecting chicken eggshell qualities. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0125890"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Song, X., Li, S., He, S., Zheng, H., Li, R., Liu, L., Geng, T., Zhao, M., and Gong, D. (2024). Integration of Whole-Genome Resequencing and Transcriptome Sequencing Reveals Candidate Genes in High Glossiness of Eggshell. Animals, 14.","DOI":"10.3390\/ani14081141"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"104692","DOI":"10.1016\/j.psj.2024.104692","article-title":"Transcriptomic insight into the underlying mechanism of induced molting on reproductive remodeling, performance and egg quality in laying hen","volume":"104","author":"Ma","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.theriogenology.2017.06.015","article-title":"Expression and localization of cyclooxygenases in the oviduct of laying hens during the ovulatory cycle","volume":"101","author":"Elhamouly","year":"2017","journal-title":"Theriogenology"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.3382\/ps.2010-01329","article-title":"Changes in prostaglandin F2alpha receptor bindings in the hen oviduct uterus before and after oviposition","volume":"90","author":"Takahashi","year":"2011","journal-title":"Poult. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"103982","DOI":"10.1016\/j.psj.2024.103982","article-title":"Effects of dietary glucosamine sulfate sodium on early laying performance and eggshell quality of laying hens","volume":"103","author":"Wang","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.3382\/ps\/pey576","article-title":"Evidences in duck (Anas platyrhynchos) by transcriptome data for supporting the biliverdin was mainly synthesized by shell gland","volume":"98","author":"Zhang","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"eRBCA-2022-1729","DOI":"10.1590\/1806-9061-2022-1729","article-title":"Identification of Genes Affected Blue Eggshell Coloration in Xuefeng Black-Bone Chickens","volume":"26","author":"Zi","year":"2024","journal-title":"Braz. J. Poult. Sci."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Li, Z., Ren, T., Li, W., Zhou, Y., Han, R., Li, H., Jiang, R., Yan, F., Sun, G., and Liu, X. (2019). Association Between the Methylation Statuses at CpG Sites in the Promoter Region of the SLCO1B3, RNA Expression and Color Change in Blue Eggshells in Lushi Chickens. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00161"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1002\/dvdy.22690","article-title":"Yolk sac endoderm is the major source of serum proteins and lipids and is involved in the regulation of vascular integrity in early chick development","volume":"240","author":"Nakazawa","year":"2011","journal-title":"Dev. Dyn."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s10930-020-09956-3","article-title":"Investigation of the Substrate-Binding Site of a Prostaglandin E Synthase in Bombyx mori","volume":"40","author":"Yamamoto","year":"2021","journal-title":"Protein. J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s12020-008-9080-z","article-title":"Cyclooxygenases expression and distribution in the normal ovary and their role in ovarian cancer in the domestic hen (Gallus domesticus)","volume":"33","author":"Hales","year":"2008","journal-title":"Endocrine"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/16\/4\/383\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:03:35Z","timestamp":1760029415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/16\/4\/383"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,27]]},"references-count":70,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["genes16040383"],"URL":"https:\/\/doi.org\/10.3390\/genes16040383","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,27]]}}}