{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:44:02Z","timestamp":1787028242441,"version":"build-2736575974"},"reference-count":74,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Teaching Reform Research in Higher Education Institutions of Jiangxi Province","award":["JXJG-23-23-5"],"award-info":[{"award-number":["JXJG-23-23-5"]}]},{"name":"Project of Teaching Reform Research in Higher Education Institutions of Jiangxi Province","award":["32160784"],"award-info":[{"award-number":["32160784"]}]},{"name":"National Natural Science Foundation of China","award":["JXJG-23-23-5"],"award-info":[{"award-number":["JXJG-23-23-5"]}]},{"name":"National Natural Science Foundation of China","award":["32160784"],"award-info":[{"award-number":["32160784"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: The testis, a critical reproductive organ in male animals, is responsible for sperm production and androgen secretion. Testis weight often correlates with reproductive performance, yet the genetic factors influencing testicular traits in chickens remain unclear. Methods: Previous genome-wide association studies (GWAS) have identified key genes affecting testicular traits in Kangle Yellow chickens, along with the associated regulatory pathways and Gene Ontology (GO) terms, through bioinformatic analyses. In this study, we utilized the existing literature, full-length transcriptome data, and proteome analyses to select key candidate genes. Results: We identified 13 associated markers for chicken testicular traits with 262 candidate genes. Nine candidate genes were found to regulate chicken testicular traits referred to integrated analysis, including CDH3, ZFPM1, CFAP52, ST6GAL1, IGF2BP2, SPG7, CDT1, NFAT5, and OPRK1. Physical interactions among these genes were also observed, implicating mechanisms such as cell adhesion molecules and neuroactive ligand\u2013receptor interaction. Conclusions: These findings provide a genetic basis for improving testicular traits in Chinese native chicken breeds.<\/jats:p>","DOI":"10.3390\/genes16060637","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T12:28:42Z","timestamp":1748435322000},"page":"637","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Novel Genetic Loci Involved in Testis Traits of the Jiangxi Local Breed Based on GWAS Analyses"],"prefix":"10.3390","volume":"16","author":[{"given":"Jing-E","family":"Ma","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Huang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7684-6331","authenticated-orcid":false,"given":"Bahareldin Ali Abdalla","family":"Gibril","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiguo","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Poultry Genetic Improvement, Nanchang Normal University, Nanchang 330032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Estermann, M.A., Major, A.T., and Smith, C.A. 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