{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T21:17:42Z","timestamp":1783718262971,"version":"3.55.0"},"reference-count":64,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2023A1515011820"],"award-info":[{"award-number":["2023A1515011820"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2024VPPC-S06"],"award-info":[{"award-number":["2024VPPC-S06"]}]},{"name":"Open Research Project of the Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control of the Ministry of Education","award":["2023A1515011820"],"award-info":[{"award-number":["2023A1515011820"]}]},{"name":"Open Research Project of the Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control of the Ministry of Education","award":["2024VPPC-S06"],"award-info":[{"award-number":["2024VPPC-S06"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: The genus Dugesia (Platyhelminthes: Tricladida) includes a large diversity of free-living freshwater flatworms and is important for studies on regeneration and evolution. This study aims to describe a newly discovered asexual planarian species from southern China and explore its genetic characteristics and regenerative abilities. Methods: An integrative taxonomic analysis was conducted using morphology, karyology, histology, molecular phylogeny (18S, 28S, COI, mitogenome), and genome size estimation via flow cytometry. Regeneration was assessed by standardized amputations, and long-term asexual propagation was observed under laboratory conditions for three years. Results: Phylogenetic analyses using nuclear (18S, 28S rDNA) and mitochondrial (COI, mitogenome) markers confirmed that Dugesia cantonensis Guang Yang &amp; Zhinan Yin, sp. nov. forms a distinct clade within Dugesia. Its 18,125 bp mitogenome contains 36 genes but lacks atp8. D. cantonensis displays a distinctive morphology, notably a pharynx located near the head. All body fragments regenerated into complete individuals within nine days. Remarkably, one individual produced ~10\u2075 clonal descendants over three years via repeated amputation, maintaining stable regenerative ability and growth across generations. Karyological analysis revealed a diploid karyotype (2n = 16) consisting of eight chromosome pairs. The nuclear genome size was estimated at approximately 2.5 Gb using Danio rerio as an internal standard. Histological examination showed no detectable reproductive organs, confirming the species as an exclusively asexual lineage. Conclusions: D. cantonensis represents a new planarian strain with stable propagation and regeneration. These features make it a valuable resource for regenerative biology and comparative genomic studies.<\/jats:p>","DOI":"10.3390\/genes16060704","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T03:53:12Z","timestamp":1749786792000},"page":"704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Integrative Taxonomy and Mitochondrial Genome Evolution of Freshwater Planarians (Platyhelminthes: Tricladida): The Discovery of a New Clade in Southern China"],"prefix":"10.3390","volume":"16","author":[{"given":"Yimeng","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0752-7917","authenticated-orcid":false,"given":"Zhizhuo","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"},{"name":"Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, China"},{"name":"Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinyi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yexin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuying","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengwen","family":"Yang","sequence":"additional","affiliation":[{"name":"The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7333-4181","authenticated-orcid":false,"given":"Chunxia","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, School of Medicine, Jinan University, Guangzhou 510632, China"},{"name":"Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhinan","family":"Yin","sequence":"additional","affiliation":[{"name":"The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1688-515X","authenticated-orcid":false,"given":"Guang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology, School of Medicine, Jinan University, Guangzhou 510632, China"},{"name":"Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"ref_1","unstructured":"Little, C. 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