{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:51:23Z","timestamp":1787698283576,"version":"build-2784847793"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: SIRT1 is a NAD+-dependent protein deacetylase regulating metabolic and stress response pathways. Genetic variations in the SIRT1 gene may contribute to the pathogenesis of type 2 diabetes mellitus (T2DM). This case\u2013control study investigates the associations of two SIRT1 promoter polymorphisms, rs12778366 and rs3758391, in patients with type 2 diabetes mellitus (T2DM), gestational diabetes mellitus (GDM), preeclampsia, and healthy controls. Methods: This case\u2013control study compared the genotypes between T2DM and pregnant and non-pregnant controls. We also compared genotypes between pregnant women with T2DM, GDM, preeclampsia, and healthy pregnant controls. Genomic DNA was extracted and analyzed using PCR-RFLP for the detection of rs12778366 and rs3758391 polymorphisms. Genotype frequencies were compared using chi-square tests, and odds ratios (ORs) with 95% confidence intervals (CIs) were calculated. Results: The study included 66 patients with T2DM, 36 with GDM, 12 with preeclampsia, and 81 pregnant and non-pregnant controls (33 pregnant controls). Although rs3758391 was more frequent in T2DM, the difference was not statistically significant between SIRT1 polymorphisms and T2DM. The CT genotype was more prevalent in T2DM (54.5%) compared to controls (33.4%); however, this difference was not significant. We finally found no significant association of the investigated SIRT1 polymorphisms with any of the conditions studied. In addition, the small sample size, especially for preeclampsia cases, limits the statistical power to detect significant associations. Conclusions: Although no significant association was observed between SIRT1 polymorphisms and diabetes, the findings of our study underscore the need for further studies examining SIRT1 polymorphisms in various ethnic groups, with a focus on leveraging these genetic variations in diabetes pathophysiology. Larger studies in the Greek population could also provide additional meaningful findings.<\/jats:p>","DOI":"10.3390\/genes16080886","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T14:50:03Z","timestamp":1753714203000},"page":"886","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Association of SIRT1 Promoter Polymorphisms with Type 2 Diabetes Mellitus and Pregnancy-Related Complications in the Greek Population"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9155-0889","authenticated-orcid":false,"given":"Sophia","family":"Letsiou","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Food Sciences, University of West Attica, 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eirini","family":"Prountzou","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Food Sciences, University of West Attica, 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9810-8308","authenticated-orcid":false,"given":"Despina","family":"Vougiouklaki","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Food Sciences, University of West Attica, 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8785-7522","authenticated-orcid":false,"given":"Maria","family":"Trapali","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, University of West Attica, 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8331-2402","authenticated-orcid":false,"given":"Michail","family":"Papapanou","sequence":"additional","affiliation":[{"name":"Second Department of Obstetrics and Gynecology, Aretaieion University Hospital, Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Siateli","sequence":"additional","affiliation":[{"name":"Medical School SAPIENZA, Universita di Roma, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Ladias","sequence":"additional","affiliation":[{"name":"Microbiological Center Life Check, 11526 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4692-2081","authenticated-orcid":false,"given":"Dimitra","family":"Houhoula","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Food Sciences, University of West Attica, 12243 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8559-8977","authenticated-orcid":false,"given":"Panagiotis","family":"Halvatsiotis","sequence":"additional","affiliation":[{"name":"2nd Propaedeutic Department of Internal Medicine, Medical School, National and Kapodistrian University of Athens, \u201cATTIKON\u201d University Hospital, 12461 Chaidari, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,27]]},"reference":[{"key":"ref_1","first-page":"supp_az_0416","article-title":"Pathophysiology of Type 2 Diabetes: The Evolution of Our Understanding","volume":"65","author":"Brunton","year":"2016","journal-title":"J. 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