{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:08:49Z","timestamp":1787314129796,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270919"],"award-info":[{"award-number":["32270919"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470927"],"award-info":[{"award-number":["32470927"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["JSSCTD202348"],"award-info":[{"award-number":["JSSCTD202348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Jiangsu Innovative and Entrepreneurial Research Team Program","award":["32270919"],"award-info":[{"award-number":["32270919"]}]},{"name":"Jiangsu Innovative and Entrepreneurial Research Team Program","award":["32470927"],"award-info":[{"award-number":["32470927"]}]},{"name":"Jiangsu Innovative and Entrepreneurial Research Team Program","award":["JSSCTD202348"],"award-info":[{"award-number":["JSSCTD202348"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: Major depressive disorder (MDD) and inflammatory bowel disease (IBD) form a \u201cbidirectional vicious cycle\u201d through the gut\u2013brain axis: psychological and emotional abnormalities can induce intestinal inflammation, while intestinal inflammation can in turn exacerbate mental health disorders. Ferroptosis is an iron-dependent form of regulated cell death that is driven by lipid peroxidation. Although this process has been molecularly defined in recent years, its role in the context of IBD and MDD remains insufficiently investigated. This study investigates the molecular roles of ferroptosis-related genes (FRGs) in both conditions and explores potential therapeutic strategies targeting these genes. Methods: We first identified differentially expressed FRGs (DE-FRGs) by comparing normal and disease samples. Subsequently, we screened for DE-FRGs in both IBD and MDD and named them Co-DEGs. Correlation analyses of these co-FRGs were performed, including comparisons between disease and control groups, as well as associations between Co-DEGs and immune cell infiltrations. Four distinct machine learning algorithms were employed to identify the core Co-DEGs associated with both IBD and MDD. Moreover, analyses of drug sensitivity, molecular docking, and molecular dynamics simulations were carried out to predict potential therapeutic agents for both conditions. Finally, single-cell sequencing analysis was also performed. Results: We identified 29 Co-DEGs in both IBD and MDD. Machine learning analysis identified RPL8 as a key common biomarker exhibiting a consistent expression trend in both diseases. A predictive approach integrating molecular docking and molecular dynamics simulations indicated that LE135, a compound targeting RPL8, is the most promising therapeutic candidate. Conclusions: These discoveries enhance the understanding of the shared and distinct regulatory mechanisms of FRGs in gut\u2013brain axis disorders. We have pinpointed key biomarkers and predicted potential therapeutic agents that may offer dual-targeting strategies for both IBD and MDD.<\/jats:p>","DOI":"10.3390\/genes16091111","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:08:58Z","timestamp":1758276538000},"page":"1111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Machine Learning Identifies Shared Regulatory Mechanisms of Genes Associated with Ferroptosis in Major Depressive Disorder and Inflammatory Bowel Disease"],"prefix":"10.3390","volume":"16","author":[{"given":"Jiyuan","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luojin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Sang","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9740-820X","authenticated-orcid":false,"given":"Liming","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China"},{"name":"Basic Medical Research Center, School of Medicine, Nantong University, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Akanyibah, F.A., He, C., Cai, P., Wang, X., Wang, Y., and Mao, F. 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