{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:40:09Z","timestamp":1760575209758,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2022-03321"],"award-info":[{"award-number":["RGPIN-2022-03321"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: The bacterium Brochothrix campestris is closely related to Brochothrix thermosphacta, a known food spoilage agent, and Listeria monocytogenes, the causative agent of listeriosis. B. campestris garnered attention several years ago because it produces brochocin-C, a bacteriocin capable of inhibiting the growth of certain pathogens. It has been recently suggested that phages play a significant role in the evolution of B. thermosphacta, similar to the role they play for L. monocytogenes. However, understanding the role of phages in the evolution of B. campestris has been challenging because only a draft of genome sequences of a single B. campestris strain was previously available. Methods: In this study, DNA from the B. campestris type strain DSM 4712 was sequenced using Oxford Nanopore and Illumina technologies to obtain complete, high-quality genome sequences. Results: The assembly revealed the presence of a plasmid and a phage-plasmid. Additionally, chromosomal analysis identified several genomic islands, including one harboring the brochocin genes, suggesting that these genes may have undergone horizontal transfer. Conclusions: This study underscores the potential importance of phages in the evolution of B. campestris, and also highlights the need for further research into the interactions between Brochothrix species and their associated phages to better understand these complex biological relationships.<\/jats:p>","DOI":"10.3390\/genes16101218","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:57:42Z","timestamp":1760529462000},"page":"1218","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Relationship Between Brochothrix campestris and Its Phages: A Cross-Replicon and Interspecies Perspective"],"prefix":"10.3390","volume":"16","author":[{"given":"Sabrina A.","family":"Att\u00e9r\u00e9","sequence":"first","affiliation":[{"name":"D\u00e9partement des Sciences Animales, Facult\u00e9 des Sciences de L\u2019agriculture et de L\u2019alimentation, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"},{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1954-9528","authenticated-orcid":false,"given":"Laurie C.","family":"Pich\u00e9","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Animales, Facult\u00e9 des Sciences de L\u2019agriculture et de L\u2019alimentation, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"},{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1338-5785","authenticated-orcid":false,"given":"Antony T.","family":"Vincent","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Animales, Facult\u00e9 des Sciences de L\u2019agriculture et de L\u2019alimentation, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"},{"name":"Institut de Biologie Int\u00e9grative et des Syst\u00e8mes, Universit\u00e9 Laval, Quebec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.fm.2018.01.015","article-title":"Genotypic and phenotypic characterization of the food spoilage bacterium Brochothrix thermosphacta","volume":"81","author":"Illikoud","year":"2019","journal-title":"Food Microbiol."},{"key":"ref_2","first-page":"99","article-title":"Brochothrix campestris sp. nov","volume":"38","author":"Talon","year":"1988","journal-title":"Int. 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