{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:29:39Z","timestamp":1760660979844,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Xinjiang Uygur Autonomous Region Major Science and Technology Special Project","award":["2024A02004"],"award-info":[{"award-number":["2024A02004"]}]},{"name":"Tianshan Talent\u00a0Training\u00a0Program high level\u00a0talents\u00a0for\u00a0the\u00a0advancement\u00a0of\u00a0modern\u00a0agriculture\u00a0and\u00a0animal\u00a0husbandry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Backgrouds: To explore the differences in semen proteins among rams of three FecB genotypes (++, B+, and BB) in Duolang sheep. Methods:\u00a0 This study employed DIA quantitative proteomics technology to identify semen proteins from four wild-type (Group A), two heterozygous (Group B), and three homozygous (Group C) rams. Results: Compared with the ++ genotype, the differentially expressed proteins (DEPs) in the semen of B+ genotype rams are significantly involved in the biological process of innate immune response and are significantly enriched in the oxidative phosphorylation pathway in KEGG analysis. From a biological perspective, the innate immune response may affect the immune health of Duolang sheep, while oxidative phosphorylation influences energy metabolism, which in turn impacts reproductive performance. Compared with the BB genotype, the DEPs in the semen of B+ genotype rams participate in biological processes such as protein phosphorylation and protein hydrolysis during cellular protein catabolism. These DEPs are also significantly enriched in pathways related to Parkinson\u2019s disease and non-alcoholic fatty liver disease in KEGG analysis. These differences may affect the cellular metabolism and physiological functions of Duolang sheep, thereby being associated with their reproductive performance. Compared with the ++ genotype, the DEPs in the semen of BB genotype rams exhibit differences in molecular function, cellular component, KEGG pathway, domain function, and subcellular localization. For instance, they are involved in threonine-type endopeptidase activity and associated with pathways like Alzheimer\u2019s disease and retrograde endocannabinoid signaling. Conclusions:\u00a0These differences may have potential impacts on the physiology and reproductive performance of Duolang sheep.<\/jats:p>","DOI":"10.3390\/genes16101226","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:33:22Z","timestamp":1760632402000},"page":"1226","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Semen Proteomic Differences Among Three Genotypes of FecB Rams in Duolang Sheep"],"prefix":"10.3390","volume":"16","author":[{"given":"Yanlong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3570-8490","authenticated-orcid":false,"given":"Zhigang","family":"Niu","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Animal Biotechnology, Key Laboratory of Herbivorous Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiabao","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengfen","family":"Xue","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Animal Biotechnology, Key Laboratory of Herbivorous Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongcai","family":"Shi","sequence":"additional","affiliation":[{"name":"Xinjiang Key Laboratory of Animal Biotechnology, Key Laboratory of Herbivorous Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"ref_1","first-page":"43","article-title":"Effect of FecB gene mutation on superovulation in sheep","volume":"45","author":"Guo","year":"2024","journal-title":"J. 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