{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:43:34Z","timestamp":1787507014757,"version":"build-2736575974"},"reference-count":134,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Vector-borne diseases account for 17% of all infectious diseases. The most effective strategies for controlling these diseases have focused on decreasing the vector population, primarily through the use of insecticides. Many insecticides have no specific targets, harming pollinators and beneficial insects. Additionally, the vector populations are developing resistance, reducing the effectiveness of these strategies and increasing ecological damage. Double-strand RNA (dsRNA) is widely used in insects to study gene function by knocking down their expression. Recently, this technology has been applied to develop RNAi-based insecticides for controlling agricultural pests. These biopesticides demonstrate high specificity, as insects do not develop resistance to them, and they cause minimal ecological damage. These pesticides knock down the expression of key genes related to vital functions, development, and reproduction, which affects the insect life cycle and consequently decreases their populations. This review focuses on using RNA interference (RNAi)-based insecticides for controlling major insect vectors, including mosquitoes, kissing bugs, and ticks. We examine the advancements and challenges associated with this technology, considering the complex life cycles and feeding behavior of these insects. Furthermore, we discuss gaps in knowledge about vector biology and delivery strategies for dsRNA, which need to be addressed to enhance the application and efficiency of this emerging technology for controlling vector-borne diseases.<\/jats:p>","DOI":"10.3390\/genes16111276","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T11:47:19Z","timestamp":1761652039000},"page":"1276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["RNAi-Based Bioinsecticides for Controlling Vector-Borne Diseases"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7810-9741","authenticated-orcid":false,"given":"Krystal","family":"Maya-Maldonado","sequence":"first","affiliation":[{"name":"Biochemistry Department, Center for Research and Advanced Studies, Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8136-883X","authenticated-orcid":false,"given":"Antonio","family":"Celestino-Montes","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Especializada en Microbiolog\u00eda, Universidad Aut\u00f3noma de Guerrero, Chilpancingo de los Bravo 39105, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4605-092X","authenticated-orcid":false,"given":"Victor","family":"Cardoso-Jaime","sequence":"additional","affiliation":[{"name":"Independent Researcher, Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2017). 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