{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:02:35Z","timestamp":1761894155320,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Biological Breeding-National Science and Technology Major Project","award":["2023ZD04053"],"award-info":[{"award-number":["2023ZD04053"]}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20240915"],"award-info":[{"award-number":["BK20240915"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"name":"earmarked fund for CARS","award":["CARS-41"],"award-info":[{"award-number":["CARS-41"]}]},{"name":"Jiangsu Key Research and Development Program","award":["BE2022341"],"award-info":[{"award-number":["BE2022341"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Avian leukosis virus subgroup J (ALV-J) harms poultry via tumors and immunosuppression; MDM2-derived circRNA-0172\u2032s role in ALV-J infection is unknown. This study explores its molecular characteristics, circularization mechanism and functions for ALV-J control. Methods: CEF\/DF-1\/HD11 cells were cultured; RNA\/DNA was extracted. circRNA-0172 was validated via PCR, sequencing and RNase R; subcellular localization and circularization were analyzed with fractionation and vectors. miRanda\/TargetScan\/GO\/KEGG, CCK-8 and qRT-PCR assessed targets, pathways and functions. Results: circRNA-0172 (1180 bp, MDM2 exons 3\u201311) is cytoplasm localized, RNase R resistant, and circularized via flanking ERVL-MaLR repeats. Highly expressed in immune tissues\/HD11 cells, it is downregulated by ALV-J. Its overexpression inhibits DF-1 proliferation, downregulates Bcl2 and upregulates CCND1. Conclusions: This is the first confirmation of circRNA-0172\u2032s stability and repeat-dependent circularization; it regulates cell proliferation\/apoptosis, providing potential targets for ALV-J-associated avian leukemia.<\/jats:p>","DOI":"10.3390\/genes16111282","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T17:38:22Z","timestamp":1761759502000},"page":"1282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characteristics, Circularization Mechanism, and Potential Functions of circRNA-0172"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1525-7584","authenticated-orcid":false,"given":"Haojie","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5738-4186","authenticated-orcid":false,"given":"Hao","family":"Bai","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4228-6540","authenticated-orcid":false,"given":"Lingling","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5712-626X","authenticated-orcid":false,"given":"Guobin","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5228","DOI":"10.1093\/nar\/gkad280","article-title":"Sex-lethal regulates back-splicing and generation of the sex-differentially expressed circular RNAs","volume":"51","author":"Fan","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.7150\/ijbs.22531","article-title":"Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity","volume":"13","author":"Wang","year":"2017","journal-title":"Int. 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