{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:47:00Z","timestamp":1773287220198,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD2401304"],"award-info":[{"award-number":["2024YFD2401304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Collaborative Innovation Project of the Zhejiang Provincial Department of Agriculture","award":["2130199"],"award-info":[{"award-number":["2130199"]}]},{"name":"Zhoushan Municipal Applied Technology Research Project","award":["2025C31022"],"award-info":[{"award-number":["2025C31022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: The sarcoplasmic reticulum Ca2+-ATPase 1 (Atp2a1) is a key regulator of calcium homeostasis and muscle relaxation, yet its roles in fish remain poorly understood. Methods: We investigated the structural characteristics, phylogenetic relationships, and transcriptional regulation of atp2a1 in Acrossocheilus fasciatus, a stream-dwelling cyprinid sensitive to environmental fluctuations. Results: Bioinformatic analyses revealed that the 991-aa Atp2a1 protein is highly conserved among teleosts but exhibits divergence from mammals in the Cation_ATPase_N domain and transmembrane regions TM3, TM9, and TM10. Phylogenetic analysis clustered A. fasciatus most closely with Onychostoma macrolepis. Tissue-specific qRT-PCR demonstrated predominant expression in skeletal muscle, followed by testis, brain, heart, and gill. Promoter prediction identified binding motifs for KLF9, CTCF, MAZ, KLF5, ONECUT3, and HOXB13. qRT-PCR analysis showed that long-term cold acclimation (16 \u00b0C vs. 24 \u00b0C) markedly downregulated atp2a1 expression (ANOVA, p &lt; 0.05, n = 3), whereas moderate flow velocity (2 BL\u00b7s\u22121 vs. 0 BL\u00b7s\u22121) significantly upregulated it (ANOVA, p &lt; 0.05, n = 3). Alternative splicing analysis based on RNA-seq data further revealed a corresponding decrease and increase in skipped exon (SE) inclusion under cold and flow conditions, respectively (Padj &lt; 0.05). Conclusions: These results further raise the possibility that the regulatory complexity of atp2a1 contributes to adaptation of teleosts under fluctuating environments.<\/jats:p>","DOI":"10.3390\/genes16111385","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T10:24:58Z","timestamp":1763375098000},"page":"1385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Structural Conservation and Transcriptional Plasticity of atp2a1 in Acrossocheilus fasciatus Under Temperature and Flow Acclimation"],"prefix":"10.3390","volume":"16","author":[{"given":"Ye","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-513X","authenticated-orcid":false,"given":"Yongyao","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316000, China"},{"name":"College of Fisheries, Zhejiang Ocean University, Zhoushan 316000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peihao","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Fisheries, Zhejiang Ocean University, Zhoushan 316000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangjie","family":"Chu","sequence":"additional","affiliation":[{"name":"College of Fisheries, Zhejiang Ocean University, Zhoushan 316000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1061-2107","authenticated-orcid":false,"given":"Bo","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Fisheries, Zhejiang Ocean University, Zhoushan 316000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.biophys.093008.131331","article-title":"P-Type ATPases","volume":"40","author":"Palmgren","year":"2011","journal-title":"Annu. 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