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While the effects of PCBs on gene expression are well-documented using short-read RNA sequencing, the regulatory roles of alternative splicing (AS) and differential transcript usage (DTU) are uncharacterized. AS has been implicated in MASLD. Previously, we reported that chronic (34 wks.) exposure of normal, low-fat-diet (LFD)-fed male mice to Ar1260 resulted in 12 hepatic RNA modifications. Proteomic analysis of these same liver samples identified Ar1260 exposure-associated changes in selenoproteins: GPX4 and SELENBP2 were increased and SELENOS and SELENOF were reduced. Methods: Here we used long-read isoform sequencing (IsoSeq) to identify DTU in four genes in the Ar1260-exposed livers: Adpgk, Blvra, Mup2, and Ndufaf6. Results: Network analysis of the corresponding proteins revealed a strong association with pathways relevant to MASLD including lipid metabolism, glycolysis, and oxidative stress. Conclusions: These findings suggest that PCB exposure alters the transcript isoform landscape of key metabolic genes involved in MASLD.<\/jats:p>","DOI":"10.3390\/genes17020126","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T08:14:23Z","timestamp":1769415263000},"page":"126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long-Read Isoform Sequencing Reveals Aroclor1260-Induced Isoform Usage in Mouse Livers"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9248-9311","authenticated-orcid":false,"given":"Belinda J.","family":"Petri","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Kentucky IDeA Networks of Biomedical Research Excellence Data Science Core, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Department of Neuroscience Training, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4132-7304","authenticated-orcid":false,"given":"Kellianne M.","family":"Piell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4356-1743","authenticated-orcid":false,"given":"Banrida","family":"Wahlang","sequence":"additional","affiliation":[{"name":"Hepatobiology and Toxicology Center, University of Louisville, Louisville, KY 40202, USA"},{"name":"The University of Louisville Superfund Research Center, Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Center for Integrative Environmental Health Sciences (CIEHS), University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6805-7220","authenticated-orcid":false,"given":"Julia H.","family":"Chariker","sequence":"additional","affiliation":[{"name":"Kentucky IDeA Networks of Biomedical Research Excellence Data Science Core, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Department of Neuroscience Training, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3487-6572","authenticated-orcid":false,"given":"Eric C.","family":"Rouchka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Kentucky IDeA Networks of Biomedical Research Excellence Data Science Core, University of Louisville School of Medicine, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.","family":"Cave","sequence":"additional","affiliation":[{"name":"Hepatobiology and Toxicology Center, University of Louisville, Louisville, KY 40202, USA"},{"name":"The University of Louisville Superfund Research Center, Division of Gastroenterology, Hepatology & Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Center for Integrative Environmental Health Sciences (CIEHS), University of Louisville, Louisville, KY 40202, USA"},{"name":"Alcohol Research Center, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3358-4378","authenticated-orcid":false,"given":"Carolyn M.","family":"Klinge","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Louisville School of Medicine, Louisville, KY 40202, USA"},{"name":"Center for Integrative Environmental Health Sciences (CIEHS), University of Louisville, Louisville, KY 40202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,25]]},"reference":[{"key":"ref_1","first-page":"101133","article-title":"A multi-society Delphi consensus statement on new fatty liver disease nomenclature","volume":"29","author":"Rinella","year":"2024","journal-title":"J. Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e2454707","DOI":"10.1001\/jamanetworkopen.2024.54707","article-title":"Estimated Burden of Metabolic Dysfunction\u2013Associated Steatotic Liver Disease in US Adults, 2020 to 2050","volume":"8","author":"Le","year":"2025","journal-title":"JAMA Netw. Open"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hirsova, P., Bamidele, A.O., Wang, H., Povero, D., and Revelo, X.S. (2021). Emerging Roles of T Cells in the Pathogenesis of Nonalcoholic Steatohepatitis and Hepatocellular Carcinoma. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.760860"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1016\/j.cell.2021.04.015","article-title":"Mechanisms and disease consequences of nonalcoholic fatty liver disease","volume":"184","author":"Loomba","year":"2021","journal-title":"Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jhep.2024.04.036","article-title":"A turning point in hepatology? EASL reflects on the first approved drug for MASH","volume":"81","author":"Francque","year":"2024","journal-title":"J. Hepatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1056\/NEJMoa2413258","article-title":"Phase 3 Trial of Semaglutide in Metabolic Dysfunction\u2013Associated Steatohepatitis","volume":"392","author":"Sanyal","year":"2025","journal-title":"N. Engl. J. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5","DOI":"10.17925\/EE.2024.20.2.2","article-title":"Metabolic Dysfunction-associated Steatotic Liver Disease and Type 2 Diabetes: A Deadly Synergy","volume":"20","author":"Leith","year":"2024","journal-title":"Touchrev Endocrinol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1111\/apt.15738","article-title":"Review article: The emerging role of genetics in precision medicine for patients with non-alcoholic steatohepatitis","volume":"51","author":"Carlsson","year":"2020","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1053\/j.gastro.2020.01.052","article-title":"NAFLD 2020: The State of the Disease","volume":"158","author":"Cotter","year":"2020","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.reprotox.2016.10.001","article-title":"Metabolism disrupting chemicals and metabolic disorders","volume":"68","author":"Heindel","year":"2017","journal-title":"Reprod. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/hep.23321","article-title":"Toxicant-associated steatohepatitis in vinyl chloride workers","volume":"51","author":"Cave","year":"2010","journal-title":"Hepatology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1055\/a-2540-2861","article-title":"Environmental Pollutants, Occupational Exposures, and Liver Disease","volume":"45","author":"Beier","year":"2025","journal-title":"Semin. Liver Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104138","DOI":"10.1016\/j.etap.2023.104138","article-title":"Disruption of the mouse liver epitranscriptome by long-term aroclor 1260 exposure","volume":"100","author":"Piell","year":"2023","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s00424-018-2136-x","article-title":"Alternative splicing isoforms in health and disease","volume":"470","author":"Kim","year":"2018","journal-title":"Pflug. Arch."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41514-020-00052-5","article-title":"Differential transcript usage unravels gene expression alterations in Alzheimer\u2019s disease human brains","volume":"7","author":"Iohan","year":"2021","journal-title":"npj Aging Mech. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1038\/s41588-025-02099-0","article-title":"Long-read RNA sequencing atlas of human microglia isoforms elucidates disease-associated genetic regulation of splicing","volume":"57","author":"Humphrey","year":"2025","journal-title":"Nat. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1016\/j.molcel.2007.07.018","article-title":"Rapid, Transcript-Specific Changes in Splicing in Response to Environmental Stress","volume":"27","author":"Pleiss","year":"2007","journal-title":"Mol. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e186478","DOI":"10.1172\/JCI186478","article-title":"Disrupted minor intron splicing activates reductive carboxylation-mediated lipogenesis to drive metabolic dysfunction\u2013associated steatotic liver disease progression","volume":"135","author":"Fu","year":"2025","journal-title":"J. Clin. Investig."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"104260","DOI":"10.1016\/j.etap.2023.104260","article-title":"Altered splicing factor and alternative splicing events in a mouse model of diet- and polychlorinated biphenyl-induced liver disease","volume":"103","author":"Petri","year":"2023","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1093\/toxsci\/kfv245","article-title":"Pyruvate Kinase Isoform Switching and Hepatic Metabolic Reprogramming by the Environmental Contaminant 2,3,7,8-Tetrachlorodibenzo-p-Dioxin","volume":"149","author":"Nault","year":"2015","journal-title":"Toxicol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"110022","DOI":"10.1016\/j.celrep.2021.110022","article-title":"Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing","volume":"37","author":"Leung","year":"2021","journal-title":"Cell Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/s41576-025-00828-z","article-title":"Transcriptomics in the era of long-read sequencing","volume":"26","author":"Liu","year":"2025","journal-title":"Nat. Rev. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/s41587-019-0217-9","article-title":"Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome","volume":"37","author":"Wenger","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.ymthe.2024.11.025","article-title":"Long-read RNA sequencing: A transformative technology for exploring transcriptome complexity in human diseases","volume":"33","author":"Ament","year":"2025","journal-title":"Mol. Ther."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Lio, C.T., D\u00fcz, T., Hoffmann, M., Willruth, L.L., Baumbach, J., List, M., and Tsoy, O. (2024). Comprehensive benchmark of differential transcript usage analysis for static and dynamic conditions. bioRxiv, bioRxiv:2024.01.14.575548.","DOI":"10.1101\/2024.01.14.575548"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1002\/hep.20701","article-title":"Design and validation of a histological scoring system for nonalcoholic fatty liver disease","volume":"41","author":"Kleiner","year":"2005","journal-title":"Hepatology"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Liang, W., Menke, A.L., Driessen, A., Koek, G.H., Lindeman, J.H., Stoop, R., Havekes, L.M., Kleemann, R., and van den Hoek, A.M. (2014). Establishment of a general NAFLD scoring system for rodent models and comparison to human liver pathology. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115922"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1007\/s00044-020-02581-w","article-title":"Dioxin-like and non-dioxin-like PCBs differentially regulate the hepatic proteome and modify diet-induced nonalcoholic fatty liver disease severity","volume":"29","author":"Jin","year":"2020","journal-title":"Med. Chem. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115726","DOI":"10.1016\/j.envpol.2020.115726","article-title":"Nontarget analysis reveals gut microbiome-dependent differences in the fecal PCB metabolite profiles of germ-free and conventional mice","volume":"268","author":"Li","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"304","DOI":"10.12688\/f1000research.23297.1","article-title":"GFF Utilities: GffRead and GffCompare","volume":"9","author":"Pertea","year":"2020","journal-title":"F1000Res"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/s41592-024-02229-2","article-title":"SQANTI3: Curation of long-read transcriptomes for accurate identification of known and novel isoforms","volume":"21","author":"Kondratova","year":"2024","journal-title":"Nat. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.12688\/f1000research.8900.2","article-title":"DRIMSeq: A Dirichlet-multinomial framework for multivariate count outcomes in genomics","volume":"5","author":"Nowicka","year":"2016","journal-title":"F1000Res"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s13059-017-1277-0","article-title":"stageR: A general stage-wise method for controlling the gene-level false discovery rate in differential expression and differential transcript usage","volume":"18","author":"Soneson","year":"2017","journal-title":"Genome Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"E5593","DOI":"10.1073\/pnas.1419161111","article-title":"rMATS: Robust and flexible detection of differential alternative splicing from replicate RNA-Seq data","volume":"111","author":"Shen","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein-protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ajcnut.2023.05.024","article-title":"Obesogens and Obesity: State-of-the-Science and Future Directions Summary from a Healthy Environment and Endocrine Disruptors Strategies Workshop","volume":"118","author":"Heindel","year":"2023","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"162920","DOI":"10.1016\/j.scitotenv.2023.162920","article-title":"Polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, pesticides, and diabetes in the Anniston Community Health Survey follow-up (ACHS II): Single exposure and mixture analysis approaches","volume":"877","author":"Pavuk","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103928","DOI":"10.1016\/j.etap.2022.103928","article-title":"Multiomics analysis of the impact of polychlorinated biphenyls on environmental liver disease in a mouse model","volume":"94","author":"Petri","year":"2022","journal-title":"Env. Toxicol. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Kaminska, D. (2024). The Role of RNA Splicing in Liver Function and Disease: A Focus on Metabolic Dysfunction-Associated Steatotic Liver Disease. Genes, 15.","DOI":"10.3390\/genes15091181"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Liu, Q., Fang, L., and Wu, C. (2022). Alternative Splicing and Isoforms: From Mechanisms to Diseases. Genes, 13.","DOI":"10.3390\/genes13030401"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1093\/toxsci\/kfv250","article-title":"Polychlorinated Biphenyl-Xenobiotic Nuclear Receptor Interactions Regulate Energy Metabolism, Behavior, and Inflammation in Non-alcoholic-Steatohepatitis","volume":"149","author":"Wahlang","year":"2016","journal-title":"Toxicol. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.semcdb.2017.08.011","article-title":"Building a complex complex: Assembly of mitochondrial respiratory chain complex I","volume":"76","author":"Formosa","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"8713","DOI":"10.3390\/ijms15058713","article-title":"Role of mitochondria in nonalcoholic fatty liver disease","volume":"15","author":"Nassir","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mej\u00eda-Guzm\u00e1n, J.E., Belmont-Hern\u00e1ndez, R.A., Ch\u00e1vez-Tapia, N.C., Uribe, M., and Nu\u00f1o-L\u00e1mbarri, N. (2025). Metabolic-Dysfunction-Associated Steatotic Liver Disease: Molecular Mechanisms, Clinical Implications, and Emerging Therapeutic Strategies. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26072959"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3694","DOI":"10.1074\/jbc.M115.679902","article-title":"The Structural and Functional Characterization of Mammalian ADP-dependent Glucokinase","volume":"291","author":"Richter","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chao, H.-W., Chao, S.-W., Lin, H., Ku, H.-C., and Cheng, C.-F. (2019). Homeostasis of Glucose and Lipid in Non-Alcoholic Fatty Liver Disease. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20020298"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Dobre, M.-Z., Virgolici, B., and Cioarc\u0103-Nedelcu, R. (2025). Lipid Hormones at the Intersection of Metabolic Imbalances and Endocrine Disorders. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47070565"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.jprot.2015.12.009","article-title":"Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse","volume":"133","author":"Vehmas","year":"2016","journal-title":"J. Proteom."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mancuso, C. (2025). The Heme Oxygenase\/Biliverdin Reductase System and Its Genetic Variants in Physiology and Diseases. Antioxidants, 14.","DOI":"10.3390\/antiox14020187"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.tem.2015.02.001","article-title":"Biliverdin reductase isozymes in metabolism","volume":"26","author":"Hosick","year":"2015","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Wegiel, B., and Otterbein, L. (2012). Go Green: The Anti-Inflammatory Effects of Biliverdin Reductase. Front. Pharmacol., 3.","DOI":"10.3389\/fphar.2012.00047"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1111\/bph.13392","article-title":"What is the evidence for the role of TRP channels in inflammatory and immune cells?","volume":"173","author":"Parenti","year":"2016","journal-title":"Br. J. Pharmacol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Wu, F., Bu, S., and Wang, H. (2024). Role of TRP Channels in Metabolism-Related Diseases. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25020692"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0083-6729(10)83006-7","article-title":"Chapter Six\u2014Major Urinary Protein Regulation of Chemical Communication and Nutrient Metabolism","volume":"Volume 83","author":"Litwack","year":"2010","journal-title":"Vitamins & Hormones"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Syed-Abdul, M.M. (2024). Lipid Metabolism in Metabolic-Associated Steatotic Liver Disease (MASLD). Metabolites, 14.","DOI":"10.3390\/metabo14010012"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.1007\/s10620-018-5242-x","article-title":"Role of the Circadian Clock in the Metabolic Syndrome and Nonalcoholic Fatty Liver Disease","volume":"63","author":"Shetty","year":"2018","journal-title":"Dig. Dis. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jhep.2024.07.034","article-title":"Targeting the liver clock improves fibrosis by restoring TGF-\u03b2 signaling","volume":"82","author":"Crouchet","year":"2024","journal-title":"J. Hepatol."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/2\/126\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T05:15:01Z","timestamp":1769490901000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/2\/126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,25]]},"references-count":58,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["genes17020126"],"URL":"https:\/\/doi.org\/10.3390\/genes17020126","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,25]]}}}