{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T12:40:44Z","timestamp":1770813644737,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Grant No.82400299"],"award-info":[{"award-number":["Grant No.82400299"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: The RNA-binding protein CELF1 is crucial for cardiac development, but its role in cardiomyocyte hypertrophy is unclear. This study investigates the effects of acute CELF1 knockdown on alternative splicing and hypertrophic growth in cardiomyocytes. Methods: Neonatal rat cardiomyocytes (NRCMs) were transfected with two siRNAs targeting CELF1. Hypertrophy was assessed by cell size and expression of hypertrophic markers via qPCR and Western blot. RNA sequencing was performed in NRCMs to identify alternative splicing events. Tead1 function was tested by knockdown in NRCMs. Selected mechanistic assays were performed primarily in HeLa cells. Results: CELF1 knockdown in NRCMs increased cardiomyocyte size and upregulated hypertrophic markers, while its overexpression restored the phenotype. RNA-seq revealed that CELF1 knockdown alters the alternative splicing pattern. Specifically, the splicing of the transcription factor Tead1 shifted from the full-length long Tead1 isoform (Tead1-L) to the exon 4-skipped short isoform (Tead1-S). In HeLa cells, CELF1 interacted with hnRNPC, an m6A reader and splicing factor, and CELF1 perturbation correlated with changes in global m6A abundance. Conclusions: These findings suggest that CELF1 regulates hypertrophic phenotypes in cardiomyocytes and is associated with alternative splicing of Tead1.<\/jats:p>","DOI":"10.3390\/genes17020159","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T16:08:17Z","timestamp":1769702897000},"page":"159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7052-135X","authenticated-orcid":false,"given":"Lingjie","family":"Hu","sequence":"first","affiliation":[{"name":"School of Medicine, Tongji University, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaili","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siying","family":"Zeng","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqiao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine, Tongji University, Shanghai 200120, China"},{"name":"Institute of Clinical Science, Fudan University, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le","family":"Ni","sequence":"additional","affiliation":[{"name":"School of Medicine, Tongji University, Shanghai 200120, China"},{"name":"School of Medicine, Shanghai Jiao Tong University, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1016\/S0140-6736(25)01637-X","article-title":"Burden of 375 diseases and injuries, risk-attributable burden of 88 risk factors, and healthy life expectancy in 204 countries and territories, including 660 subnational locations, 1990\u20132023: A systematic analysis for the Global Burden of Disease Study 2023","volume":"406","author":"Hay","year":"2025","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1016\/S0140-6736(25)01917-8","article-title":"Global burden of 292 causes of death in 204 countries and territories and 660 subnational locations, 1990\u20132023: A systematic analysis for the Global Burden of Disease Study 2023","volume":"406","author":"Naghavi","year":"2025","journal-title":"Lancet"},{"key":"ref_3","first-page":"IV90","article-title":"Neurohumoral activation in preclinical heart failure. 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