{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:25:54Z","timestamp":1785432354897,"version":"3.56.0"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013805","name":"Shanxi Agricultural University","doi-asserted-by":"publisher","award":["2023BQ109"],"award-info":[{"award-number":["2023BQ109"]}],"id":[{"id":"10.13039\/501100013805","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2023BQ109"],"award-info":[{"award-number":["2023BQ109"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Objectives: Low-temperature stress has become a key factor severely restricting seedling growth in wheat, highlighting the growing importance of research on low-temperature tolerance in wheat. Most previous studies focused on the aboveground organs, and only a minority specifically targeted the roots. Methods: In this study, a recombinant inbred line population derived from a cross between DH118 and Jinmai 919 was used to dissect the genetic regulatory mechanism underlying root growth and development in wheat seedlings under low-temperature conditions. Results: A total of 9 QTLs associated with low-temperature tolerance coefficient were identified; among them, QCi.saw-2B and QCi.saw-5B were validated to be significantly associated with cold tolerance in a natural population including 289 wheat accessions. Moreover, 13 candidate genes from QTL intervals regulating root growth and development under low-temperature stress were further identified based on RNA sequencing. Conclusions: These results provide a foundation for further exploring the regulatory mechanism of root growth and development in wheat under low-temperature stress.<\/jats:p>","DOI":"10.3390\/genes17040447","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:38:26Z","timestamp":1776080306000},"page":"447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["QTL Mapping and RNA-Seq Revealed Loci and Candidate Genes Associated with Cold Tolerance of Wheat Root at Seedling Stage"],"prefix":"10.3390","volume":"17","author":[{"given":"Xuanshang","family":"Liu","sequence":"first","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University, Jinzhong 030800, China"},{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyan","family":"Si","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University, Jinzhong 030800, China"},{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwei","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Agriculture, Shanxi Agricultural University, Jinzhong 030800, China"},{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Qiao","sequence":"additional","affiliation":[{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingwei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3700-0042","authenticated-orcid":false,"given":"Jiajia","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00122-024-04802-x","article-title":"Genetic dissection of flag leaf morphology traits and fine mapping of a novel QTL (Qflw.sxau-6BL) in bread wheat (Triticum aestivum L.)","volume":"138","author":"Qiao","year":"2025","journal-title":"Theor. 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