{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T05:35:27Z","timestamp":1777008927979,"version":"3.51.4"},"reference-count":26,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFF0710500"],"award-info":[{"award-number":["2022YFF0710500"]}]},{"name":"National Key Research and Development Program of China","award":["2023YFF0724603"],"award-info":[{"award-number":["2023YFF0724603"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: Cataract is a progressive lens opacity. According to the World Health Organization, about 45 million people in the world are blind, with about half of these cases attributable to cataracts. Due to the complexity of cataract disease, current research on cataracts is far from sufficient, so it is especially important to understand the development process and the pathogenic factors of cataracts. Myodes rufocanus (M. rufocanus) is an animal of the M. rufocanus of the hamster family Volinae. In developing M. rufocanus, we found an individual of M. rufocanus with a congenital cataract phenotype. We confirmed the symptoms of cataract under natural light and using a slit lamp. Methods: Therefore, we analyzed the mechanism of congenital cataract in M. rufocanus from the aspects of pathological histology, physiology and biochemistry, and gene level, aiming to explore the feasibility of its development into an animal model of cataract. Cataract is a progressive lens opacity and a leading cause of visual impairment. Understanding its pathogenesis requires appropriate animal models. In a laboratory-bred colony of M. rufocanus, we identified individuals with a spontaneous congenital cataract phenotype, confirmed by gross observation and slit lamp examination. To characterize this phenotype and explore its potential as an animal model, we performed pathological, physiological, biochemical, and transcriptomic analyses using three cataract-affected and three normal age-matched male individuals (8 weeks old per group). Results: Blood tests revealed significantly lower white blood cell and lymphocyte counts in the cataract group, while blood glucose and other biochemical parameters showed no significant differences. Histologically, cataractous lenses exhibited eosinophilic aggregation in the nuclear region with disorganized fiber cells. Transcriptome analysis identified 6544 differentially expressed genes, including downregulation of crystallin genes (CRYBB2, CRYBA4, CRYGS) known to be associated with congenital cataract. KEGG pathway enrichment analysis highlighted retinol metabolism, tyrosine metabolism, and cytochrome P450-related pathways. RT-qPCR confirmed reduced CRYBB2 expression in cataractous eyes. Conclusions: This study provides the first transcriptome dataset for M. rufocanus ocular tissues and offers preliminary evidence that this naturally occurring cataract phenotype may serve as a potential model for congenital cataract research.<\/jats:p>","DOI":"10.3390\/genes17050495","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T09:01:24Z","timestamp":1776848484000},"page":"495","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Myodes rufocanus Cataract Identification and Transcriptome Analysis"],"prefix":"10.3390","volume":"17","author":[{"given":"Mingzhe","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuyun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengnan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulu","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0589-2178","authenticated-orcid":false,"given":"Xianfeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fushi","family":"Quan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi\u2019an Road, Changchun 130062, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/ceo.13892","article-title":"Cataract and systemic disease: A review","volume":"49","author":"Ang","year":"2021","journal-title":"Clin. Exp. Ophthalmol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wang, D., Tang, T., Li, P., Zhao, J., Shen, B., and Zhang, M. (2024). The global burden of cataracts and its attributable risk factors in 204 countries and territories: A systematic analysis of the global burden of disease study. Front. Public Health, 12.","DOI":"10.3389\/fpubh.2024.1366677"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.18240\/ijo.2023.09.21","article-title":"Time trends and gender disparities of Chinese cataract burden and their predictions","volume":"16","author":"Wu","year":"2023","journal-title":"Int. J. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"260","DOI":"10.7589\/2014-02-053","article-title":"Isolation of Pathogenic Yersinia enterocolitica 1B\/O:8 from Apodemus Mice in Japan","volume":"51","author":"Oda","year":"2014","journal-title":"J. Wildl. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"99","DOI":"10.3106\/041.036.0208","article-title":"A Note on the Genetic Status of the Dark Red-Backed Vole, Myodes rex, in Hokkaido, Japan","volume":"36","author":"Iwasa","year":"2011","journal-title":"Mammal Study"},{"key":"ref_6","first-page":"15","article-title":"Humoral Immune Response of Overwintered Gray Red-Backed Voles (Myodes rufocanus bedfordiae) under Cold Stress in Spring","volume":"100","author":"Kusumoto","year":"2015","journal-title":"Bull. Fac. Agric. Saga Univ."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Soininen, E.M., Valentini, A., Coissac, E., Miquel, C., Gielly, L., Brochmann, C., Brysting, A.K., S\u00f8nsteb\u00f8, J.H., Ims, R.A., and Yoccoz, N.G. (2009). Analysing diet of small herbivores: The efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures. Front. Zool., 6.","DOI":"10.1186\/1742-9994-6-16"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3106\/041.039.0204","article-title":"Ag-NORs Analysis in Three Species of Red-Backed Voles, with a Consideration of Generic Allocation of Anderson\u2019s Red-Backed Vole","volume":"39","author":"Suzuki","year":"2014","journal-title":"Mammal Study"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1387\/ijdb.041854jg","article-title":"Congenital hereditary cataracts","volume":"48","author":"Graw","year":"2004","journal-title":"Int. J. Dev. Biol."},{"key":"ref_10","first-page":"165","article-title":"Analysis of disease-causing gene mutation in three Chinese families with congenital inherited cataract","volume":"35","author":"Ma","year":"2018","journal-title":"Zhonghua Yi Xue Yi Chuan Xue Za Zhi"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.atherosclerosis.2015.02.031","article-title":"Clinical and genetic analysis of a family diagnosed with familial hypobetalipoproteinemia in which the proband was diagnosed with diabetes mellitus","volume":"239","author":"Wang","year":"2015","journal-title":"Atherosclerosis"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e8024","DOI":"10.15252\/msb.20178024","article-title":"Timing of gene expression in a cell-fate decision system","volume":"14","author":"Aymoz","year":"2018","journal-title":"Mol. Syst. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3069","DOI":"10.1093\/bioinformatics\/btv313","article-title":"DSigDB: Drug signatures database for gene set analysis","volume":"31","author":"Yoo","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ferraz, A.L., Ojeda, A., L\u00f3pez-B\u00e9jar, M., Fernandes, L.T., Castell\u00f3, A., Folch, J.M., and P\u00e9rez-Enciso, M. (2008). Transcriptome architecture across tissues in the pig. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-173"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"170185","DOI":"10.1038\/sdata.2017.185","article-title":"An RNA-Seq atlas of gene expression in mouse and rat normal tissues","volume":"4","author":"Leparc","year":"2017","journal-title":"Sci. Data"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gluck, C., Min, S., Oyelakin, A., Smalley, K., Sinha, S., and Romano, R.-A. (2016). RNA-seq based transcriptomic map reveals new insights into mouse salivary gland development and maturation. BMC Genom., 17.","DOI":"10.1186\/s12864-016-3228-7"},{"key":"ref_17","first-page":"1019","article-title":"A missense mutation in CRYBA4 associated with congenital cataract and microcornea","volume":"16","author":"Zhou","year":"2010","journal-title":"Mol. Vis."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Jiao, X., Kabir, F., Irum, B., Khan, A.O., Wang, Q., Li, D., Khan, A.A., Husnain, T., Akram, J., and Riazuddin, S. (2016). A Common Ancestral Mutation in CRYBB3 Identified in Multiple Consanguineous Families with Congenital Cataracts. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0157005"},{"key":"ref_19","first-page":"356","article-title":"Transcriptome profiling of differentiated lenses through RNA sequencing","volume":"56","author":"Zhang","year":"2020","journal-title":"Zhonghua Yan Ke Za Zhi"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Guo, R., Huang, D., Ji, J., and Liu, W. (2022). A novel mutation GJA8 NM_005267.5: C.124G > A, p.(E42K) causing congenital nuclear cataract. BMC Ophthalmol., 22.","DOI":"10.1186\/s12886-022-02386-y"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hassan, A.Y., Yousaf, S., Levin, M.R., Saeedi, O.J., Riazuddin, S., Alexander, J.L., and Ahmed, Z.M. (2021). Novel Homozygous Missense Variant in GJA3 Connexin Domain Causing Congenital Nuclear and Cortical Cataracts. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010240"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/humu.23395","article-title":"Mutation update of transcription factor genes FOXE3, HSF4, MAF, and PITX3 causing cataracts and other developmental ocular defects","volume":"39","author":"Anand","year":"2018","journal-title":"Hum. Mutat."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5694","DOI":"10.1128\/MCB.24.13.5694-5709.2004","article-title":"Synergistic transcription activation by Maf and Sox and their subnuclear localization are disrupted by a mutation in Maf that causes cataract","volume":"24","author":"Rajaram","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"ref_24","first-page":"1146","article-title":"Comparison of pseudophakic retinal straylight in spherical\/aspherical and hydrophobic\/hydrophilic intraocular lens","volume":"8","author":"Tang","year":"2015","journal-title":"Int. J. Ophthalmol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1080\/13816810.2018.1484930","article-title":"A novel MIP mutation in a Chinese family with congenital cataract","volume":"39","author":"Yuan","year":"2018","journal-title":"Ophthalmic Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.gene.2019.01.011","article-title":"A novel MAF missense mutation leads to congenital nuclear cataract by impacting the transactivation of crystallin and noncrystallin genes","volume":"692","author":"Si","year":"2019","journal-title":"Gene"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/5\/495\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T04:37:09Z","timestamp":1777005429000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/5\/495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,22]]},"references-count":26,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["genes17050495"],"URL":"https:\/\/doi.org\/10.3390\/genes17050495","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,22]]}}}