{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:38:15Z","timestamp":1785418695672,"version":"3.56.0"},"reference-count":24,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470549"],"award-info":[{"award-number":["32470549"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32470549"],"award-info":[{"award-number":["32470549"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei","doi-asserted-by":"crossref","award":["2021CFB510"],"award-info":[{"award-number":["2021CFB510"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Hubei International Scientific and Technological Cooperation Base of Ecology and Environment","award":["GH202501"],"award-info":[{"award-number":["GH202501"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Mosquitoes, including Culex quinquefasciatus and Aedes aegypti, are important vectors of dengue fever, Zika virus, West Nile virus, Japanese encephalitis virus, Eastern equine encephalitis virus, etc. Biological control has always been urgent in mosquito prevention due to resistance developing to synthetic insecticides and environmental toxicity by insecticides. Methods: The leaf essential oil of Mosla. chinensis was isolated, and major components were identified via GC-MS, followed by olfactory behavior assays to evaluate its repellent activity against C. quinquefasciatus. Additionally, the odorant-binding protein 1 and odorant-binding protein 2 (CquiOBP1-2) genes were prokaryotically expressed, and their fluorescence competitive binding activities with the active components of essential oils were examined. Results: The bioassays indicated this essential oil greatly repels C. quinquefasciatus, which will significantly protect people against vector-borne diseases. In the fluorescence competitive binding experiments, the CquiOBP1-2 proteins exhibit great binding capacities to volatile components, including Citronellal, Citronellol, Geraniol, Limonene and Isopulegol. Furthermore, the behavioral experimental results also indicate that the mixture of these five ligand compounds has an obvious repellent effect on mosquitoes, highlighting that they may be applied as potential mosquito repellent agents. Moreover, molecular docking and site-directed mutation analysis further confirm Phe123 and Gln77 are both key amino acid residues of CquiOBP1-2 proteins involved in the olfactory recognition of repellent ligand compounds from M. chinensis essential oil. Conclusions: The behavioral experimental verification and the exploration of olfactory molecular mechanisms are helpful to promote the biological control of plant essential oils in mosquito pests.<\/jats:p>","DOI":"10.3390\/genes17060707","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T14:37:42Z","timestamp":1781879862000},"page":"707","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["OBP-Mediated Molecular Mechanism Underlying the Olfactory Repellent Effect of Mosla chinensis Essential Oil Against Culex quinquefasciatus"],"prefix":"10.3390","volume":"17","author":[{"given":"Jinfeng","family":"Xiong","sequence":"first","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Ai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University, Wuhan 430079, China"},{"name":"Academy of Frontier Interdisciplinary Research, Central China Normal University, Wuhan 430079, China"},{"name":"Research Center for Territorial Spatial Conservation, Utilization and Computational Governance, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1007\/s00436-011-2593-z","article-title":"Larvicidal activity of micronized aqueous suspension of calcium hydroxide against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)","volume":"110","author":"Estrada","year":"2012","journal-title":"Parasitol. 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