{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:18:24Z","timestamp":1785417504510,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Research Project of Fuyang Normal University","award":["2025FSKJ31"],"award-info":[{"award-number":["2025FSKJ31"]}]},{"award":["2025FSKJ31"],"award-info":[{"award-number":["2025FSKJ31"]}],"id":[{"id":"https:\/\/ror.org\/02njz9p87","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Doctoral Talent Introduction Project of Fuyang Normal University","award":["2020KYQD0031"],"award-info":[{"award-number":["2020KYQD0031"]}]},{"award":["2020KYQD0031"],"award-info":[{"award-number":["2020KYQD0031"]}],"id":[{"id":"https:\/\/ror.org\/02njz9p87","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Major Project of Anhui Huatuo Academy of Traditional Chinese Medicine","award":["BZKZ2419"],"award-info":[{"award-number":["BZKZ2419"]}]},{"name":"Distinguished Young Project of Natural Science Foundation of Universities of Anhui Province","award":["2022AH020081"],"award-info":[{"award-number":["2022AH020081"]}]},{"name":"Central Financial Forestry Science and Technology Extension Demonstration Project","award":["Wan [2026] TG09-2"],"award-info":[{"award-number":["Wan [2026] TG09-2"]}]},{"name":"Key Project of Anhui Provincial University Scientific Research Project","award":["2023AH050420"],"award-info":[{"award-number":["2023AH050420"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background:\u00a0Pectobacterium brasiliense-mediated soft rot severely threatens the production of diverse cash crops worldwide and brings severe yield reduction risks. A virulent strain BS1113 was separated from diseased cigar tobacco plants collected in Yunnan, yet its virulence regulatory genes and copper resistance-related genetic background have not been fully analyzed so far. This study aims to decipher the genomic features of BS1113 and clarify its pathogenic and copper-tolerant characteristics via whole-genome sequencing, comparative genomics and indoor phenotype verification. Methods: Hybrid sequencing strategies combining Illumina short reads and PacBio long reads were adopted to obtain the complete circular genome sequence of strain BS1113. Subsequent comparative genomic analysis and multiple phenotypic identification experiments were conducted to characterize its genetic architecture and physiological traits. Results: Genome assembly results showed that the circular chromosome of BS1113 spans 4,916,962 bp with a GC content of 51.96%, which encodes a total of 4369 functional protein-coding genes. Genomic comparison revealed that BS1113 completely lacks the T3SS gene cluster, while it conserves intact T2SS, T6SS and I-F CRISPR-Cas systems; the chromosomal copper resistance operon copRSAB was also detected in this isolate. Pathogenicity tests validated that BS1113 satisfies all criteria of Koch\u2019s postulates on cigar tobacco hosts. In addition, BS1113 displayed prominent tolerance against eight mainstream copper bactericides widely used for tobacco disease management. Conclusions: This research generates the first complete high-quality genome of P. brasiliense isolated from cigar tobacco hosts. The genomic data explain the infection mechanism of this pathogen independent of intact T3SS, and also reveal the genetic basis supporting its persistent survival under long-term copper fungicide pressure in field cultivation environments.<\/jats:p>","DOI":"10.3390\/genes17070775","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:06:34Z","timestamp":1782875194000},"page":"775","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Complete Genome Analysis of Pectobacterium brasiliense BS1113, a Causal Agent of Cigar Tobacco Soft Rot, with Phenotypic Characterization of Virulence and Copper Tolerance"],"prefix":"10.3390","volume":"17","author":[{"given":"Xuemei","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Hu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuting","family":"Geng","sequence":"additional","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Li","sequence":"additional","affiliation":[{"name":"Joint Research Center for Chinese Herbal Medicine of Anhui ofIHM, Bozhou Vocational and Technical College, Bozhou 236800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Davidsson, P.R., Kariola, T., Niemi, O., and Palva, E.T. 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