{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:06:35Z","timestamp":1792523195569,"version":"4.5.0"},"reference-count":85,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Project of the Education of Anhui Province","award":["2024AH051725"],"award-info":[{"award-number":["2024AH051725"]}]},{"name":"Scientific Research Project of the Education of Anhui Province","award":["2025AHGXZK31157"],"award-info":[{"award-number":["2025AHGXZK31157"]}]},{"DOI":"10.13039\/501100017588","name":"Horizontal Research Fund Project of Huainan Normal University","doi-asserted-by":"publisher","award":["2025HX413"],"award-info":[{"award-number":["2025HX413"]}],"id":[{"id":"10.13039\/501100017588","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025HX413"],"award-info":[{"award-number":["2025HX413"]}],"id":[{"id":"https:\/\/ror.org\/03n7a5z57","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Both NPs and Cd alone exert adverse effects on plant growth by disrupting physiological processes and gene expression. However, the mechanisms underlying their combined effects on plant genetic responses remain incompletely understood. Methods: The rice seedlings were used as the experimental material, with the following six treatments established: CK (Control, no NPs and Cd), 10 and 100 mg\u00b7L\u22121 NPs, and 0.5 mg\u00b7L\u22121 Cd alone and combination. Seedlings at the three-leaf stage were treated in hydroponic culture for 7 days, after which root-development parameters, root viability, MDA and soluble sugar contents, and SOD and POD activities were measured, along with transcriptomic analysis. Results: The results show that root length, number of root tips, and root surface area were highest in CK compared with all NPs and Cd treatments, particularly. Significant differences were observed between the CK and both the high-concentration NPs and all Cd treatments groups (p &lt; 0.05). Root growth progressively declined with increasing NP concentrations; the combination of 100 mg\u00b7L\u22121 NPs and 0.5 mg\u00b7L\u22121 Cd exhibited synergistic toxicity, which decreased root length, number of root tips, and root surface area by 14%, 22%, and 4% compared with 0.5 Cd, whereas 10 mg\u00b7L\u22121 NPs significantly alleviated Cd-induced root damage for the three root parameters above in the following order: 0.5 Cd &lt; 0.5 Cd-10 NPs &lt; 10 NPs. In terms of physiological changes, 10 mg\u00b7L\u22121 NPs reduced MDA levels and enhanced SOD and POD activities in roots exposed to Cd; in contrast, 100 mg\u00b7L\u22121 NPs exacerbated Cd-induced membrane peroxidation and decreased SOD and POD activities. In the high-concentration NPs and all Cd-treated groups, all aforementioned indicators exhibited significant differences compared with the CK (p &lt; 0.05). Transcriptomic and WGCNA analyses revealed that the expression levels of OsGRP162 (regulating resistance) and OsCYP2 (inhibiting lateral root formation under overexpression) were significantly higher in 0.5 Cd-100 NPs compared with the other five treatments, while OsTubA2 (positively regulating root length) exhibited a different pattern. Differentially expressed genes (DEGs) in experimental groups of 10 NPS_vs_0.5 Cd-10 NPS and 100 NPS_vs_0.5 Cd-100 NPS were predominantly enriched in glutathione metabolism and the MAPK signaling pathway, respectively. The key genes OsMT4C, OsMT4B and OsYSL2 (associated with transmembrane signal transduction), and OsABCB5 and OsCUL1-3 (involved in negative regulation of root elongation) exhibited reduced expression levels in 0.5 Cd-10 NPs, whereas OsYDA2 and OsAGO1c (related to antioxidant defense) showed upregulated expression. Conversely, the opposite gene expression patterns were observed in 0.5 Cd-100 NPs. Conclusions: These findings demonstrate that both NPs and Cd adversely affect rice seedlings; however, low concentrations of NPs mitigate Cd toxicity, while high concentrations exacerbate it. Therefore, to prevent elevated NP concentrations in plant growth environments, plastic usage and processing should be standardized.<\/jats:p>","DOI":"10.3390\/genes17070835","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T16:16:39Z","timestamp":1784650599000},"page":"835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic Changes and Physiological Genetic Responses of Oryza sativa L. Roots Under Stress of Nanoplastics (NPs) and Cadmium (Cd) in Single and Combination Forms"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4953-1211","authenticated-orcid":false,"given":"Haitao","family":"Liu","sequence":"first","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"},{"name":"Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyu","family":"Lv","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingwen","family":"Gong","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingling","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological Engineering, Huainan Normal University, Huainan 232038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"768297","DOI":"10.3389\/fmicb.2021.768297","article-title":"Environmental impacts of microplastics and nanoplastics: A current overview","volume":"12","author":"Amobonye","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"140200","DOI":"10.1016\/j.jhazmat.2025.140200","article-title":"Polyethylene plastic degradation: The dual pathways from macroplastics to nanoplastics","volume":"499","author":"Kharraf","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"117154","DOI":"10.1016\/j.ecoenv.2024.117154","article-title":"A comprehensive risk assessment of microplastics in soil, water, and atmosphere: Implications for human health and environmental safety","volume":"285","author":"Wang","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"117391","DOI":"10.1016\/j.trac.2023.117391","article-title":"A critical review of biodegradable plastic mulch films in agriculture: Definitions, scientific background and potential impacts","volume":"170","author":"Campanale","year":"2024","journal-title":"TrAC-Trend. Anal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/s41565-023-01534-9","article-title":"Microplastic fragmentation by rotifers in aquatic ecosystems contributes to global nanoplastic pollution","volume":"19","author":"Zhao","year":"2023","journal-title":"Nat. Nanotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100628","DOI":"10.1016\/j.impact.2026.100628","article-title":"Integrating approaches and regulations to address plastic pollution: Spotlighting nanosized particles","volume":"42","author":"Novak","year":"2026","journal-title":"NanoImpact"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"120267","DOI":"10.1016\/j.envres.2024.120267","article-title":"Environmental behavior and toxic effects of micro (nano) plastics and engineered nanoparticles on marine organisms under ocean acidification: A review","volume":"263","author":"Liu","year":"2024","journal-title":"Environ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.trac.2018.10.025","article-title":"Micro-(nano) plastics in freshwater ecosystems: Abundance, toxicological impact and quantification methodology","volume":"110","author":"Strungaru","year":"2019","journal-title":"TrAC-Trend. Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127784","DOI":"10.1016\/j.chemosphere.2020.127784","article-title":"Nanoplastic occurrence in a soil amended with plastic debris","volume":"262","author":"Wahl","year":"2021","journal-title":"Chemosphere"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1016\/j.envpol.2018.01.024","article-title":"Current opinion: What is a nanoplastic?","volume":"235","author":"Gigault","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"127863","DOI":"10.1016\/j.jclepro.2021.127863","article-title":"Micro- and nanoplastics in the environment: Occurrence, detection, characterization and toxicity\u2014A critical review","volume":"313","author":"Ali","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"167672","DOI":"10.1016\/j.scitotenv.2023.167672","article-title":"Macro, meso, micro and nanoplastics in horticultural soils in Argentina: Abundance, size distribution and fragmentation mechanism","volume":"906","author":"Berenstein","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104529","DOI":"10.1016\/j.pce.2026.104529","article-title":"Hidden particles: Abundance, chemical characteristics, metal binding properties, and environmental risk of microplastics in the downstream of riverine sediment-water ecosystems","volume":"114","author":"Mahapatra","year":"2026","journal-title":"Phys. Chem. Earth"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"118491","DOI":"10.1016\/j.trac.2025.118491","article-title":"Micro (nano) plastic detection in soils: Analytical techniques, AI applications, and future challenges","volume":"194","author":"Huang","year":"2025","journal-title":"TrAC-Trend. Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"127723","DOI":"10.1016\/j.envpol.2026.127723","article-title":"Nanoplastics in soil and aquatic ecosystems: Sources, impacts, and nature-based remediation strategies","volume":"395","author":"Kaya","year":"2026","journal-title":"Environ. Pollut."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"172951","DOI":"10.1016\/j.scitotenv.2024.172951","article-title":"Micro- and nanoplastics in agricultural soils: Assessing impacts and navigating mitigation","volume":"931","author":"Seo","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"104357","DOI":"10.1016\/j.pce.2026.104357","article-title":"Evaluating the multidimensional impacts of micro- and nanoplastics on soil properties, yield, and quality of horticultural crops","volume":"143","author":"Kavalackal","year":"2026","journal-title":"Phys. Chem. Earth"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"179538","DOI":"10.1016\/j.scitotenv.2025.179538","article-title":"Occurrence and environmental consequences of microplastics and nanoplastics from agricultural reuse of wastewater and biosolids in the soil ecosystem: A review","volume":"980","author":"Carpanez","year":"2025","journal-title":"Sci. Total Environ."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"124237","DOI":"10.1016\/j.envres.2026.124237","article-title":"Systemic distribution and transplacental transfer of micro- and nanoplastics in a filter-feeding marine predator","volume":"298","author":"Salvia","year":"2026","journal-title":"Environ. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"113642","DOI":"10.1016\/j.envres.2022.113642","article-title":"Micro (nano) plastics and plastic additives effects in marine annelids: A literature review","volume":"214","author":"Pires","year":"2022","journal-title":"Environ. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"123543","DOI":"10.1016\/j.envres.2025.123543","article-title":"High levels of microplastic and nanoplastic contamination in birds from remote Himalayan forests","volume":"291","author":"Zhou","year":"2025","journal-title":"Environ. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"107586","DOI":"10.1016\/j.psep.2025.107586","article-title":"Microplastics and nanoplastics across the food web: Challenges and mitigation strategies in securing human health","volume":"201","author":"Chen","year":"2025","journal-title":"Process Saf. Environ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8487","DOI":"10.2147\/IJN.S170723","article-title":"Potential adverse effects of nanoparticles on the reproductive system","volume":"13","author":"Wang","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"120517","DOI":"10.1016\/j.envpol.2022.120517","article-title":"Role of heteroaggregation and internalization in the toxicity of differently sized and charged plastic nanoparticles to freshwater microalgae","volume":"316","author":"Li","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"130725","DOI":"10.1016\/j.chemosphere.2021.130725","article-title":"Toxic effects of polystyrene nanoplastics on microalgae Chlorella vulgaris: Changes in biomass, photosynthetic pigments and morphology","volume":"280","author":"Khoshnamvand","year":"2021","journal-title":"Chemosphere"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s12951-023-01830-5","article-title":"Advances in transport and toxicity of nanoparticles in plants","volume":"21","author":"Gao","year":"2023","journal-title":"J. Nanobiotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"121620","DOI":"10.1016\/j.jhazmat.2019.121620","article-title":"Impact of polystyrene nanoplastics (PSNPs) on seed germination and seedling growth of wheat (Triticum aestivum L.)","volume":"385","author":"Lian","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"142363","DOI":"10.1016\/j.jhazmat.2026.142363","article-title":"Biodegradable nanoplastics pose a greater risk: Polylactic acid exceeds polystyrene in phytotoxicity and bioaccumulation in lettuce","volume":"512","author":"Li","year":"2026","journal-title":"J. Hazard. Mater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"124540","DOI":"10.1016\/j.envres.2026.124540","article-title":"Cross-regional assessment of heavy metal contamination and Cd exceedance risk in specialty vegetable-producing soils across China","volume":"301","author":"Wang","year":"2026","journal-title":"Environ. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wang, F., Wang, Z.H., Kou, C.L., Ma, Z.H., and Zhao, D. (2016). Responses of wheat yield, macro- and micro-nutrients, and heavy metals in soil and wheat following the application of manure compost on the north China plain. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146453"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"125275","DOI":"10.1016\/j.watres.2025.125275","article-title":"Impact of fertilizers on polyethylene terephthalate nanoplastics migration in groundwater: A combined experimental and simulation approach","volume":"291","author":"Cai","year":"2025","journal-title":"Water Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"100245","DOI":"10.1016\/j.clwas.2025.100245","article-title":"Potential use of sewage sludge as fertilizer in organic farming","volume":"10","author":"Balkrishna","year":"2025","journal-title":"Clean. Waste Syst."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"133643","DOI":"10.1016\/j.scitotenv.2019.133643","article-title":"Adsorption behavior of organic pollutants and metals on micro\/nanoplastics in the aquatic environment","volume":"694","author":"Yu","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.scitotenv.2016.01.029","article-title":"Magnetite nanoparticle (NP) uptake by wheat plants and its effect on cadmium and chromium toxicological behavior","volume":"565","year":"2016","journal-title":"Sci. Total Environ."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"114498","DOI":"10.1016\/j.envpol.2020.114498","article-title":"Do polystyrene nanoplastics affect the toxicity of cadmium to wheat (Triticum aestivum L.)?","volume":"263","author":"Lian","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"138758","DOI":"10.1016\/j.jhazmat.2025.138758","article-title":"Translocation of polystyrene nanoplastics in distinct plant species: Novel insight from a split-root system and transcriptomic analysis","volume":"494","author":"Xu","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"119339","DOI":"10.1016\/j.watres.2022.119339","article-title":"Negative impacts of nanoplastics on the purification function of submerged plants in constructed wetlands: Responses of oxidative stress and metabolic processes","volume":"227","author":"Liu","year":"2022","journal-title":"Water Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"123412","DOI":"10.1016\/j.jhazmat.2020.123412","article-title":"Response of rice (Oryza sativa L.) roots to nanoplastic treatment at seedling stage","volume":"401","author":"Zhou","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"142188","DOI":"10.1016\/j.scitotenv.2020.142188","article-title":"Cadmium stress in paddy fields: Effects of soil conditions and remediation strategies","volume":"754","author":"Hussain","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9925","DOI":"10.1021\/acs.est.3c01134","article-title":"Role of residual monomers in the manifestation of (cyto)toxicity by polystyrene microplastic model particles","volume":"57","author":"Zhang","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"ref_41","first-page":"194","article-title":"Investigation and evaluation of typical heavy metal elements in soil around mining subsidence area","volume":"47","author":"Wang","year":"2019","journal-title":"Int. J. Coal Sci. Technol."},{"key":"ref_42","unstructured":"Li, X.F., Sun, Y., and Zhang, W. (2025). Experiment Guidance of Plant Physiology, Higher Education Press."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s00128-022-03659-4","article-title":"Oxidative damage in roots of rice (Oryza sativa L.) seedlings exposed to microplastics or combined with Cadmium","volume":"110","author":"Liu","year":"2023","journal-title":"Bull. Environ. Contam. Tox."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"126791","DOI":"10.1016\/j.chemosphere.2020.126791","article-title":"Interactions of microplastics and cadmium on plant growth and arbuscular mycorrhizal fungal communities in an agricultural soil","volume":"254","author":"Wang","year":"2020","journal-title":"Chemosphere"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.ecoenv.2018.06.034","article-title":"Carboxylatedmulti-walled carbon nanotubes exacerbated oxidative damage in roots of Vicia faba L. seedlings under combined stress of lead and cadmium","volume":"161","author":"Rong","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"129128","DOI":"10.1016\/j.jhazmat.2022.129128","article-title":"Integrated physiological, transcriptomic and metabolomic analysis of the response of Trifolium pratense L. to Pb toxicity","volume":"436","author":"Meng","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chi, W.C., Chen, Y.A., Hsiung, Y.C., Fu, S.F., Chou, C.H., Trinh, N.N., Chen, Y.C., and Huang, H.J. (2013). Autotoxicity mechanism of Oryza sativa: Transcriptome response in rice roots exposed to ferulic acid. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-351"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"Feature Counts: An efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with deseq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.3389\/fpls.2017.01432","article-title":"Integrating early transcriptomic responses to rhizotoxins in rice (Oryza sativa. L.) reveals key regulators and a potential early biomarker of cadmium toxicity","volume":"8","author":"Huang","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1242\/dev.090548","article-title":"To branch or not to branch: The role of pre-patterning in lateral root formation","volume":"140","author":"Xuan","year":"2013","journal-title":"Development"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00128-024-03899-6","article-title":"A study on the growth and physiological toxicity effects of the combined exposure of microplastics and cadmium on the Vicia faba L. Seedlings","volume":"112","author":"Wang","year":"2024","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"127241","DOI":"10.1016\/j.jhazmat.2021.127241","article-title":"Nanotoxicological effects and transcriptome mechanisms of wheat (Triticum aestivum L.) under stress of polystyrene nanoplastics","volume":"423","author":"Lian","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.plaphy.2019.01.007","article-title":"Cadmium tolerance is associated with the root-driven coordination of cadmium sequestration, iron regulation, and ROS scavenging in rice","volume":"136","author":"Bari","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"166434","DOI":"10.1016\/j.scitotenv.2023.166434","article-title":"Under flooding conditions, controlled-release fertiliser coated microplastics affect the growth and accumulation of cadmium in rice by increasing the ffuidity of cadmium and interfering with metabolic pathways","volume":"901","author":"Zhao","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"127531","DOI":"10.1016\/j.jhazmat.2021.127531","article-title":"Effects of microplastics on soil properties: Current knowledge and future perspectives","volume":"424","author":"Wang","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_57","first-page":"25","article-title":"Current research status of woody plants responding to drought stress","volume":"60","author":"Ma","year":"2023","journal-title":"J. Sichuan Univ. (Nat. Sci. Ed.)"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"8896","DOI":"10.1038\/s41598-023-36119-y","article-title":"Morpho-physiological responses and growth indices of triticale to drought and salt stresses","volume":"13","author":"Alagoz","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"108795","DOI":"10.1016\/j.plaphy.2024.108795","article-title":"Micro and nanoplastics pollution: Sources, distribution, uptake in plants, toxicological effects, and innovative remediation strategies for environmental sustainability","volume":"213","author":"Kumar","year":"2024","journal-title":"Plant Physiol. Bioch."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"124373","DOI":"10.1016\/j.envpol.2024.124373","article-title":"Polystyrene nanoplastics distinctly impact cadmium uptake and toxicity in Arabidopsis thaliana","volume":"356","author":"Li","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1093\/aob\/mcm156","article-title":"Oxidative stress and antioxidants in tomato (Solanum lycopersic) plants Subjected to Boron Toxicity","volume":"100","author":"Cervilla","year":"2007","journal-title":"Ann. Bot."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"113892","DOI":"10.1016\/j.envpol.2019.113892","article-title":"Microplastic particles increase arsenic toxicity to rice seedlings","volume":"259","author":"Dong","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"13394","DOI":"10.1007\/s11356-020-10388-w","article-title":"Polystyrene nanoplastics affect growth and microcystin production of Microcystis aeruginosa","volume":"28","author":"Zheng","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"124335","DOI":"10.1016\/j.chemosphere.2019.07.066","article-title":"Evaluation of single and combined effects of cadmium and microplastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio)","volume":"236","author":"Banaee","year":"2019","journal-title":"Chemosphere"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"115315","DOI":"10.1016\/j.ecoenv.2023.115315","article-title":"Root system architecture and genomic plasticity to salinity provide insights into salt-tolerant traits in tall fescue","volume":"262","author":"Fan","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.4161\/psb.22455","article-title":"Reactive oxygen species generation and signaling in plants","volume":"7","author":"Tripathy","year":"2012","journal-title":"Plant Signal. Behav."},{"key":"ref_67","first-page":"246","article-title":"Expression of bowman-birk trypsin inhibitor at different development stages in rice leaves and seed germination","volume":"20","author":"Xu","year":"2012","journal-title":"J. Agric. Biotechnol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.scib.2023.11.046","article-title":"Diurnal regulation of alternative splicing associated with thermotolerance in rice by two glycine-rich RNA-binding proteins","volume":"69","author":"Yang","year":"2024","journal-title":"Sci. Bull."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.cj.2025.02.006","article-title":"The rice OsCBL3-OsCIPK31 module regulates root development via abscisic acid and auxin signaling pathways","volume":"13","author":"Yu","year":"2025","journal-title":"Crop J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1360\/02yc0172","article-title":"Structure, expression characteristics and chromosomal localization of the rice OSgrp-2 Gene","volume":"46","author":"Liu","year":"2003","journal-title":"Sci. China Ser. C Life Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"112367","DOI":"10.1016\/j.postharvbio.2023.112367","article-title":"Cold shock treatment delays broccoli flower bud yellowing by repressing the gene expression of glycine-rich RNA-binding protein 2, a senescence-associated protein involved in the regulation of stomata opening","volume":"201","author":"Hsiao","year":"2023","journal-title":"Postharvest Biol. Technol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.plantsci.2018.10.019","article-title":"Functional screening of salt tolerance genes from a halophyte Sporobolus virginicus and transcriptomic and metabolomic analysis of salt tolerant plants expressing glycine-rich RNA-binding protein","volume":"278","author":"Tada","year":"2019","journal-title":"Plant Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.plantsci.2013.10.006","article-title":"Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Oryza sativa) under drought stress conditions","volume":"214","author":"Yang","year":"2014","journal-title":"Plant Sci."},{"key":"ref_74","first-page":"4","article-title":"Oxidative signaling response to cadmium exposure","volume":"156","author":"Nemmiche","year":"2017","journal-title":"Toxicol. Sci."},{"key":"ref_75","first-page":"1","article-title":"Research advance on action mode and application of antimicrobial peptides from insects","volume":"31","author":"Osama","year":"2014","journal-title":"Chem. Bioeng."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"984","DOI":"10.3390\/plants13070984","article-title":"The impact of nanomaterials on photosynthesis and antioxidant mechanisms in gramineae plants: Research progress and future prospects","volume":"13","author":"Li","year":"2024","journal-title":"Plants"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2476","DOI":"10.1111\/pce.13843","article-title":"OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice","volume":"43","author":"Chang","year":"2020","journal-title":"Plant Cell Environ."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"148173","DOI":"10.1016\/j.gene.2024.148173","article-title":"A long non-coding RNA associated with H3K7me3 methylation negatively regulates OsZIP16 transcription under cadmium stress","volume":"901","author":"Li","year":"2024","journal-title":"Gene"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s00299-024-03303-x","article-title":"Metal transport proteins and transcription factor networks in plant responses to cadmium stress","volume":"43","author":"Liu","year":"2024","journal-title":"Plant Cell Rep."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"32534","DOI":"10.1038\/srep32534","article-title":"Purple foliage coloration in tea (Camellia sinensis L.) arises from activation of the R2R3-MYB transcription factor CsAN1","volume":"6","author":"Sun","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"132758","DOI":"10.1016\/j.jhazmat.2023.132758","article-title":"Multi-omics analysis reveals differential molecular responses to cadmium toxicity in rice root tip and mature zone","volume":"462","author":"Kuang","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"134086","DOI":"10.1016\/j.jhazmat.2024.134086","article-title":"Mechanistic insight into the intensification of arsenic toxicity to rice (Oryza sativa L.) by nanoplastic: Phytohormone and glutathione metabolism modulation","volume":"469","author":"Jiang","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"4100","DOI":"10.1038\/s41467-025-59449-z","article-title":"Suppressing an auxin efflux transporter enhances rice adaptation to temperate habitats","volume":"16","author":"Cui","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1016\/j.molp.2025.07.011","article-title":"A novel OsCRK14\u2013OsRLCK57\u2013MAPK signaling module activates OsbZIP66 to confer drought resistance in rice","volume":"18","author":"Ye","year":"2025","journal-title":"Mol. Plant"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s10142-023-01061-9","article-title":"OsMBTB32, a MATH-BTB domain-containing protein that interacts with OsCUL1s to regulate salt tolerance in rice","volume":"23","author":"Ullah","year":"2023","journal-title":"Funct. Integr. Genom."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/7\/835\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T16:22:31Z","timestamp":1784650951000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/7\/835"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":85,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["genes17070835"],"URL":"https:\/\/doi.org\/10.3390\/genes17070835","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]}}}