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Most mechanistic studies emphasize mutations in the pore-forming voltage-gated sodium channels \u03b1-subunit, whereas the contribution of insect-specific sodium-channel auxiliary subunits remains unclear. Methods: We used dsRNA feeding, pyrethroid bioassays, RNA sequencing, and RT-qPCR validation to evaluate the role of temperature-induced paralytic E (TipE) in M. usitatus. Results: The optimized RNAi condition was 400 \u03bcg\/mL of dsTipE for 48 h, which reduced TipE expression by 32.55%. TipE knockdown increased adult mortality after exposure to \u03bb-cyhalothrin and permethrin, indicating increased pyrethroid-associated susceptibility under laboratory conditions. Transcriptome sequencing identified 205 differentially expressed genes, with enrichment in metabolism-related pathways. RT-qPCR confirmed reduced expression of CYP307a1, an ecdysteroid-biosynthesis-related P450 gene, and SCD, a lipid-metabolism gene, while Nav \u03b1-subunit transcript abundance did not change significantly. Conclusions: These results identify TipE as an auxiliary-subunit factor associated with pyrethroid susceptibility in M. usitatus and provide a transcriptome-based working model linking sodium-channel auxiliary regulation with endocrine- and lipid-metabolism-related responses. This study expands the candidate space for pyrethroid resistance research beyond Nav \u03b1-subunit mutations, although the causal roles of CYP307a1 and SCD require further validation.<\/jats:p>","DOI":"10.3390\/genes17080854","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T07:36:13Z","timestamp":1784878573000},"page":"854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNAi-Mediated Knockdown of the Sodium Channel Auxiliary Subunit TipE Increases Pyrethroid-Associated Mortality and Reveals Metabolic Transcriptional Responses in Megalurothrips usitatus"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9813-9419","authenticated-orcid":false,"given":"Likui","family":"Wang","sequence":"first","affiliation":[{"name":"Sanya Institute, China Agricultural University, Sanya 572024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7799-3448","authenticated-orcid":false,"given":"Linlin","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3683-1001","authenticated-orcid":false,"given":"Zhengke","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-8918","authenticated-orcid":false,"given":"Pei","family":"Liang","sequence":"additional","affiliation":[{"name":"Sanya Institute, China Agricultural University, Sanya 572024, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1167-3986","authenticated-orcid":false,"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4695-5390","authenticated-orcid":false,"given":"Shaoying","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572024, China"},{"name":"School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571700, China"},{"name":"School of Tropical Fruits and Vegetables, Hainan University, Baoting 572300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Occurrence patterns of major insect pests on cowpea and evaluation of cowpea varieties resistant to Megalurothrips usitatus in Hainan","volume":"16","author":"Zhang","year":"2025","journal-title":"J. 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