{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:00:35Z","timestamp":1785423635733,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Citrus sinensis, Olea europaea var. sylvestris, Populus nigra, Prunus dulcis, and Punica granatum are economically and medicinally important perennial plant species. Carotenoid cleavage oxygenase (CCO) genes encode key enzymes involved in carotenoid degradation and play essential roles in plant growth, development, and responses to environmental stresses. In this study, a comprehensive genome-wide comparative analysis of the CCO gene family was conducted in C. sinensis, O. europaea var. sylvestris, P. nigra, P. dulcis, and P. granatum to investigate their structural diversity, evolutionary relationships, and potential biological functions. Methods: Chromosomal distribution, phylogenetic relationships, gene structure, conserved protein motifs, homology modeling, subcellular localization, cis-regulatory elements, and miRNA interactions were analyzed. Results: A total of 12, 23, 22, 11, and 17 CCO genes were identified in C. sinensis, O. europaea var. sylvestris, P. nigra, P. dulcis, and P. granatum, respectively. Most CCO proteins were acidic, and genes were concentrated on specific chromosomes. Phylogenetic analysis grouped CCO genes into three main clades. Gene structure analysis revealed intronless and intron-containing genes of varying lengths. Some CCO proteins possessed all conserved motifs, while others lacked certain motifs or had multiple copies. \u03b2-sheets were the predominant secondary structural elements, and CCO proteins were predicted to be localized in chloroplasts, mitochondria, peroxisomes, the cytoplasm, and the nucleus. Stress-related cis-elements and miRNAs were identified. Conclusions: These findings provide valuable insights into the diversity and evolutionary characteristics of the CCO gene family and suggest that CCO genes may contribute to plant stress responses and metabolic processes. Overall, this study provides a comprehensive comparative analysis of the CCO gene family in these five perennial plant species and offers a valuable genomic resource for future functional characterization, comparative genomic studies, and molecular breeding applications.<\/jats:p>","DOI":"10.3390\/genes17080903","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:55:11Z","timestamp":1785419711000},"page":"903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unraveling the Phylogenetic, Structural, and Functional Dynamics of CCO Genes in Citrus sinensis, Olea europaea var. sylvestris, Populus nigra, Prunus dulcis, and Punica granatum: A Comprehensive Bioinformatic Comparative Analysis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0281-1048","authenticated-orcid":false,"given":"Ummahan","family":"\u00d6z","sequence":"first","affiliation":[{"name":"Department of Plant and Animal Production, Manisa Celal Bayar University, 45600 Manisa, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1080\/13102818.2020.1824618","article-title":"Genome-Wide Identification and Expression Analysis of the Carotenoid Metabolic Pathway Genes in Pepper (Capsicum annuum L.)","volume":"34","author":"Guo","year":"2020","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Haider, M.Z., Sami, A., Shafiq, M., Anwar, W., Ali, S., Ali, Q., Muhammad, S., Manzoor, I., Shahid, M.A., and Ali, D. (2023). Genome-Wide Identification and In Silico Expression Analysis of Carotenoid Cleavage Oxygenases Gene Family in Oryza sativa (Rice) in Response to Abiotic Stress. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1269995"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.plaphy.2017.12.001","article-title":"Genome-Wide Analysis of Carotenoid Cleavage Oxygenase Genes and Their Responses to Various Phytohormones and Abiotic Stresses in Apple (Malus domestica)","volume":"123","author":"Chen","year":"2018","journal-title":"Plant Physiol. Biochem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.plaphy.2021.02.041","article-title":"Genome-Wide Identification, Characterization and Expression Analysis of the Carotenoid Cleavage Oxygenase (CCO) Gene Family in Saccharum","volume":"162","author":"Su","year":"2021","journal-title":"Plant Physiol. Biochem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yao, Y., Jia, L., Cheng, Y., Ruan, M., Ye, Q., Wang, R., Yao, Z., Zhou, G., Liu, J., and Yu, J. (2022). Evolutionary Origin of the Carotenoid Cleavage Oxygenase Family in Plants and Expression of Pepper Genes in Response to Abiotic Stresses. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.792832"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s11105-021-01284-9","article-title":"Genome-Wide Identification and Expression Analysis of the Carotenoid Cleavage Oxygenase Gene Family in Five Rosaceae Species","volume":"39","author":"Zhao","year":"2021","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113652","DOI":"10.1016\/j.scienta.2024.113652","article-title":"Genome-Wide Identification of Carotenoid Cleavage Oxygenase Genes in Orah Mandarin and the Mechanism by Which CrCCD4b1 Affects Peel Color","volume":"338","author":"Wen","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"108253","DOI":"10.1016\/j.plaphy.2023.108253","article-title":"Genome-Wide Analysis of Carotenoid Cleavage Oxygenases and Identification of Ripening-Associated DzNCED5a in Durian (Durio zibethinus) Fruit","volume":"206","author":"Tantisuwanichkul","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Xue, G., Hu, L., Zhu, L., Chen, Y., Qiu, C., Fan, R., Ma, X., Cao, Z., Chen, J., and Shi, J. (2023). Genome-Wide Identification and Expression Analysis of CCO Gene Family in Liriodendron chinense. Plants, 12.","DOI":"10.3390\/plants12101975"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1007\/s00344-015-9520-y","article-title":"Genome-Wide Classification and Abiotic Stress-Responsive Expression Profiling of Carotenoid Oxygenase Genes in Brassica rapa and Brassica oleracea","volume":"35","author":"Kim","year":"2016","journal-title":"J. Plant Growth Regul."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Naeem, S., Wang, Y., Han, S., Khan, M.H., Ilyas, M., Shah, M.K.N., Ali, M., Ali, M.A., Ahmad, P., and Ahmad, N. (2024). Genome-Wide Analysis and Identification of Carotenoid Cleavage Oxygenase (CCO) Gene Family in Coffee (Coffea arabica) under Abiotic Stress. BMC Genom. Data, 25.","DOI":"10.1186\/s12863-024-01248-4"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ahmad, A., Sami, A., Habib, U., Ali, M., Shafiq, M., Haider, M.Z., Ahmad, S., Ali, B., Harakeh, S., and Makki, R.M. (2024). Genome-Wide Analysis of Carotenoid Cleavage Oxygenases (CCO) Gene Family in Arachis hypogaea (Peanut) under Biotic Stress. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10351-w"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Akram, J., Siddique, R., Shafiq, M., Tabassum, B., Manzoor, M.T., Javed, M.A., Anwar, S., Nisa, B.U., Saleem, M.H., and Javed, B. (2023). Genome-Wide Identification of CCO Gene Family in Cucumber (Cucumis sativus) and Its Comparative Analysis with A. thaliana. BMC Plant Biol., 23.","DOI":"10.1186\/s12870-023-04647-4"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s13353-021-00634-3","article-title":"Genome-Wide Characterization of Carotenoid Oxygenase Gene Family in Three Cotton Species and Functional Identification of GaNCED3 in Drought and Salt Stress","volume":"62","author":"Cai","year":"2021","journal-title":"J. Appl. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/s11103-024-01433-0","article-title":"Genome-Wide Identification and In Silico Expression Analysis of CCO Gene Family in Sunflower (Helianthus annuus) against Abiotic Stress","volume":"114","author":"Sami","year":"2024","journal-title":"Plant Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4737","DOI":"10.1007\/s11033-013-2570-y","article-title":"Genome-Wide Identification and Transcription Analysis of Soybean Carotenoid Oxygenase Genes during Abiotic Stress Treatments","volume":"40","author":"Wang","year":"2013","journal-title":"Mol. Biol. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Yu, J., Wang, Y., Bai, H., Zhang, X., and Wang, R. (2024). Genome-Wide Identification and Expressional Analysis of Carotenoid Cleavage Oxygenase (CCO) Gene Family in Betula platyphylla under Abiotic Stress. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10777-2"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yue, X.Q., Zhang, Y., Yang, C.K., Li, J.G., Rui, X., Ding, F., Hu, F.C., Wang, X.H., Ma, W.Q., and Zhou, K.B. (2022). Genome-Wide Identification and Expression Analysis of Carotenoid Cleavage Oxygenase Genes in Litchi (Litchi chinensis Sonn.). BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03772-w"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"100272","DOI":"10.1016\/j.prmcm.2023.100272","article-title":"Botanical Description, Chemical Composition, Traditional Uses and Pharmacology of Citrus sinensis: An Updated Review","volume":"8","author":"Dongre","year":"2023","journal-title":"Pharmacol. Res. Mod. Chin. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"40748","DOI":"10.1007\/s11356-023-31526-0","article-title":"Chemical Composition and Antioxidant and Antibacterial Properties of the Essential Oils Extracted from Citrus sinensis Peels by Hydrodistillation and Microwave Methods","volume":"31","author":"Hacib","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fanelli, V., Mascio, I., Falek, W., Miazzi, M.M., and Montemurro, C. (2022). Current Status of Biodiversity Assessment and Conservation of Wild Olive (Olea europaea L. subsp. europaea var. sylvestris). Plants, 11.","DOI":"10.3390\/plants11040480"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kyriakopoulou, C.I., and Kalogianni, D.P. (2020). Genetic Identification of the Wild Form of Olive (Olea europaea var. sylvestris) Using Allele-Specific Real-Time PCR. Foods, 9.","DOI":"10.3390\/foods9040467"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100880","DOI":"10.1016\/j.scp.2022.100880","article-title":"Phytochemistry, Chemical Composition and Therapeutic Uses of Populus nigra L. Aerial Parts from 1991\u20132021 Onwards: An Overview","volume":"30","author":"Tebbi","year":"2022","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kis, B., Avram, S., Pavel, I.Z., Lombrea, A., Buda, V., Dehelean, C.A., Soica, C., Yerer, M.B., Bojin, F., and Folescu, R. (2020). Recent Advances Regarding the Phytochemical and Therapeutic Uses of Populus nigra L. Buds. Plants, 9.","DOI":"10.3390\/plants9111464"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ben Khadher, T., Sassi-Aydi, S., Aydi, S., Mars, M., and Bouajila, J. (2023). Phytochemical Profiling and Biological Potential of Prunus dulcis Shell Extracts. Plants, 12.","DOI":"10.20944\/preprints202306.0680.v1"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11295-023-01588-9","article-title":"Genome-Wide Association Analysis and Validation with KASP Markers for Nut and Shell Traits in Almond (Prunus dulcis [Mill.] D.A. Webb)","volume":"19","author":"Sideli","year":"2023","journal-title":"Tree Genet. Genomes"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.lwt.2015.08.076","article-title":"Phytochemical Investigations and Evaluation of Antidiabetic Potential of Prunus dulcis Nuts","volume":"66","author":"Qureshi","year":"2016","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"113877","DOI":"10.1016\/j.jep.2021.113877","article-title":"A Unique Understanding of Traditional Medicine of Pomegranate, Punica granatum L. and Its Current Research Status","volume":"271","author":"Ge","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"113256","DOI":"10.1016\/j.biopha.2022.113256","article-title":"Medicinal Uses, Pharmacological Activities, Phytochemistry, and the Molecular Mechanisms of Punica granatum L. (Pomegranate) Plant Extracts: A Review","volume":"153","author":"Maphetu","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jan, R., Asaf, S., Numan, M., and Kim, K.M. (2021). Plant Secondary Metabolite Biosynthesis and Transcriptional Regulation in Response to Biotic and Abiotic Stress Conditions. Agronomy, 11.","DOI":"10.3390\/agronomy11050968"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (2005). Protein Identification and Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook, Humana Press.","DOI":"10.1385\/1592598900"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s43897-021-00020-x","article-title":"MG2C: A User-Friendly Online Tool for Drawing Genetic Maps","volume":"1","author":"Chao","year":"2021","journal-title":"Mol. Hortic."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree of Life (iTOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1093\/bioinformatics\/btu817","article-title":"GSDS 2.0: An Upgraded Gene Feature Visualization Server","volume":"31","author":"Hu","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"W39","DOI":"10.1093\/nar\/gkv416","article-title":"The MEME Suite","volume":"43","author":"Bailey","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 Web Portal for Protein Modeling, Prediction and Analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"W585","DOI":"10.1093\/nar\/gkm259","article-title":"WoLF PSORT: Protein Localization Predictor","volume":"35","author":"Horton","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: A Web Tool for Visualizing Clustering of Multivariate Data Using Principal Component Analysis and Heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a Database of Plant Cis-Acting Regulatory Elements and a Portal to Tools for In Silico Analysis of Promoter Sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W155","DOI":"10.1093\/nar\/gkr319","article-title":"psRNATarget: A Plant Small RNA Target Analysis Server","volume":"39","author":"Dai","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"D155","DOI":"10.1093\/nar\/gky1141","article-title":"miRBase: From microRNA Sequences to Function","volume":"47","author":"Kozomara","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Shaffique, S., Hussain, S., Kang, S.M., Imran, M., Injamum-Ul-Hoque, M., Khan, M.A., and Lee, I.J. (2023). Phytohormonal Modulation of the Drought Stress in Soybean: Outlook, Research Progress, and Cross-Talk. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1237295"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.bbagrm.2011.05.001","article-title":"Role of miRNAs and siRNAs in Biotic and Abiotic Stress Responses of Plants","volume":"1819","author":"Khraiwesh","year":"2012","journal-title":"Biochim. Biophys. Acta Gene Regul. Mech."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.tplants.2012.01.010","article-title":"Functions of microRNAs in Plant Stress Responses","volume":"17","author":"Sunkar","year":"2012","journal-title":"Trends Plant Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.bbagrm.2008.04.004","article-title":"The Role of microRNAs and Other Endogenous Small RNAs in Plant Stress Responses","volume":"1779","author":"Shukla","year":"2008","journal-title":"Biochim. Biophys. Acta Gene Regul. Mech."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s13562-022-00770-9","article-title":"RNA- and miRNA-Interference to Enhance Abiotic Stress Tolerance in Plants","volume":"31","author":"Tiwari","year":"2022","journal-title":"J. Plant Biochem. Biotechnol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S1672-0229(11)60022-3","article-title":"Plant Small RNAs: Biogenesis, Mode of Action and Their Roles in Abiotic Stresses","volume":"9","author":"Guleria","year":"2011","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zhang, F., Yang, J., Zhang, N., Wu, J., and Si, H. (2022). Roles of microRNAs in Abiotic Stress Response and Characteristics Regulation of Plant. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.919243"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.1093\/jxb\/ert164","article-title":"Emerging Roles of microRNAs in the Mediation of Drought Stress Response in Plants","volume":"64","author":"Ding","year":"2013","journal-title":"J. Exp. Bot."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Sarwar, S., Sami, A., Haider, M.Z., Tasawar, L., Akram, J., Ahmad, A., Shafiq, M., Zaki, H.E.M., Ondrasek, G., and Shahid, M.S. (2025). Genome-Wide Identification and In Silico Expression Analysis of CCO Gene Family in Citrus clementina (Citrus) in Response to Abiotic Stress. Plants, 14.","DOI":"10.3390\/plants14020249"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Cheng, C., Yang, R., Yin, L., Zhang, J., Gao, L., Lu, R., Yang, Y., Wang, P., Mu, X., and Zhang, S. (2023). Characterization of Carotenoid Cleavage Oxygenase Genes in Cerasus humilis and Functional Analysis of ChCCD1. Plants, 12.","DOI":"10.3390\/plants12112114"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Kiraga, J., Mackiewicz, P., Mackiewicz, D., Kowalczuk, M., Biecek, P., Polak, N., Smolarczyk, K., Dudek, M.R., and Cebrat, S. (2007). The Relationships between the Isoelectric Point and Length of Proteins, Taxonomy and Ecology of Organisms. BMC Genom., 8.","DOI":"10.1186\/1471-2164-8-163"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.1038\/s41598-020-59981-6","article-title":"Machine Learning Improves Our Knowledge about miRNA Functions towards Plant Abiotic Stresses","volume":"10","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Shriram, V., Kumar, V., Devarumath, R.M., Khare, T.S., and Wani, S.H. (2016). MicroRNAs As Potential Targets for Abiotic Stress Tolerance in Plants. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00817"}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/8\/903\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:00:22Z","timestamp":1785420022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/8\/903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":55,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["genes17080903"],"URL":"https:\/\/doi.org\/10.3390\/genes17080903","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}