{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:45Z","timestamp":1786978425202,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wuhan Municipal Health Commission","award":["WX23Z52"],"award-info":[{"award-number":["WX23Z52"]}]},{"award":["WX23Z52"],"award-info":[{"award-number":["WX23Z52"]}],"id":[{"id":"https:\/\/ror.org\/04jjn5s14","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Objectives: Heart failure (HF) arises from multiple interrelated pathological processes. Among these, lysosomal impairment and loss of autophagic homeostasis are increasingly recognized as important contributors to myocardial damage and ventricular remodeling. This study sought to identify lysophagy-associated signature genes in HF and to define their biological roles, cellular origins, and potential diagnostic relevance. Methods: Bulk myocardial transcriptome datasets, including GSE16499, GSE57338, and GSE76701, were integrated with the human cardiac single-cell dataset GSE145154. Differential expression analysis was first performed to identify lysophagy-related differentially expressed genes (DEGs). Candidate hub genes were then screened using support vector machine-recursive feature elimination (SVM-RFE) and least absolute shrinkage and selection operator (LASSO) regression. Functional enrichment analysis, Gene Set Enrichment Analysis (GSEA), immune infiltration assessment, single-cell transcriptomic mapping, and regulatory network analysis were subsequently conducted. The expression profiles of the selected genes were validated in a murine HF model, and VAMP8 overexpression assays were performed in H9c2 cells. Results: Five hub genes, namely VAMP8, STX2, MCOLN1, DERL1, and PTP4A2, were consistently and markedly decreased in failing myocardial tissue. These genes were mainly linked to SNARE-dependent vesicle trafficking and lysophagy regulation. A diagnostic model incorporating these hub genes demonstrated good discriminatory performance in both the training dataset and a small independent validation cohort, supporting further evaluation of their potential diagnostic value. Single-cell analysis further indicated that these genes were primarily enriched in cardiac FOLR2+ tissue-resident macrophages (TRMs). Pseudotime and cell\u2013cell communication analyses associated this module with FOLR2+ TRM cell states and predicted interactions with cardiac stromal cells. In the HF mouse model, the mRNA levels of all five hub genes were decreased, with concurrent reductions in VAMP8, MCOLN1 and DERL1 protein expression. In Ang II\/LLOMe-induced H9c2 cells, VAMP8 overexpression was associated with reduced cardiomyocyte injury, attenuation of changes in the abundance of lysosome- and autophagy-related proteins, and fewer ultrastructural abnormalities, suggesting a potential cardioprotective effect. Conclusions: VAMP8, STX2, MCOLN1, DERL1, and PTP4A2 were identified as candidate molecular markers of HF that reflect alterations in a lysophagy- and vesicular-transport-related program associated with FOLR2+ tissue-resident macrophages. These findings provide new insights into immune-microenvironment remodeling in HF and suggest potential directions for mechanistic and therapeutic investigations.<\/jats:p>","DOI":"10.3390\/genes17080957","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:42:17Z","timestamp":1786974137000},"page":"957","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Bulk and Single-Cell Transcriptomic Analyses Identify a FOLR2+ Tissue-Resident Macrophage-Associated Lysophagy Gene Module in Heart Failure"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0627-5043","authenticated-orcid":false,"given":"Qi","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0768-6684","authenticated-orcid":false,"given":"Yanli","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Jianghan University, Wuhan 430056, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8151-0941","authenticated-orcid":false,"given":"Deqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Jianghan University, Wuhan 430056, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2934-3638","authenticated-orcid":false,"given":"Guoxing","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Medicine, Jianghan University, Wuhan 430056, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biyun","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1201-7036","authenticated-orcid":false,"given":"Qien","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5569-4538","authenticated-orcid":false,"given":"Fen","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e396","DOI":"10.1016\/S2214-109X(23)00557-0","article-title":"Characteristics, management, and outcomes in women and men with congestive heart failure in 40 countries at different economic levels: An analysis from the Global Congestive Heart Failure (G-CHF) registry","volume":"12","author":"Joseph","year":"2024","journal-title":"Lancet Glob. 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