{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T10:16:13Z","timestamp":1788171373823,"version":"build-2803163510"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gansu Academy of Agricultural Sciences Doctoral Fund","award":["2024GAAS30"],"award-info":[{"award-number":["2024GAAS30"]}]},{"name":"Gansu Provincial Department of Agriculture and Rural Affairs Science and Technology Support Program","award":["KJZC-2026-27"],"award-info":[{"award-number":["KJZC-2026-27"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260483"],"award-info":[{"award-number":["32260483"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32260483"],"award-info":[{"award-number":["32260483"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background: Glutathione S-transferases (GSTs) play pivotal roles in plant growth, abiotic stress responses, detoxification of xenobiotics, and maintenance of redox homeostasis. Methods: In this study, transcriptomic analysis was employed to identify differentially expressed genes (DEGs) in the pea cultivar DX27 under freezing stress (\u22124 \u00b0C) at 3, 6, and 12 h. Results:GO and KEGG enrichment analyses of global transcriptomic data further reveal that DEGs are significantly enriched in the following functional categories: oxidation\u2013reduction processes, stress responses, glutathione metabolism, and secondary metabolite biosynthesis. A total of 52 PsatGST genes were identified and classified into nine subfamilies based on phylogenetic relationships. Chromosomal localization revealed a non-random distribution across seven chromosomes. Promoter cis-element analysis indicated that PsatGST genes harbor diverse regulatory elements associated with light responsiveness, hormone signaling (auxin, abscisic acid, gibberellin, salicylic acid, and MeJA), and abiotic\/biotic stress responses. PsatGSTF2 expression analysis showed pronounced up-regulation under freezing stress (\u22124 \u00b0C) at 3, 6, and 12 h. Conclusions: These findings provide a foundational framework for understanding the evolutionary history and functional diversification of the PsatGST gene family and offer valuable candidate genes for the breeding of stress-tolerant pea varieties.<\/jats:p>","DOI":"10.3390\/genes17091052","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T09:21:19Z","timestamp":1788168079000},"page":"1052","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification of the PsatGST Gene Family and Expression Analysis Under Freezing Stress in Peas (Pisum sativum L.)"],"prefix":"10.3390","volume":"17","author":[{"given":"Zaoxia","family":"Niu","sequence":"first","affiliation":[{"name":"Institute of Crop Science, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Crop Science, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Bolin","family":"Sun","sequence":"additional","affiliation":[{"name":"Zhangye City Economic Crop Technology Promotion Station, Zhangye 734000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Gengmei","family":"Min","sequence":"additional","affiliation":[{"name":"Institute of Crop Science, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Zongwen","family":"Chai","sequence":"additional","affiliation":[{"name":"Gansu Provincial General Station of Agricultural Technology Extension, Lanzhou 730020, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Yang","family":"Shao","sequence":"additional","affiliation":[{"name":"Linxia Hui Autonomous Prefecture Academy of Agricultural Sciences, Linxia 731100, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0131","DOI":"10.1199\/tab.0131","article-title":"Glutathione transferases","volume":"8","author":"Dixon","year":"2010","journal-title":"Arab. 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