{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T05:12:53Z","timestamp":1788671573231,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["075-15-2025-487"],"award-info":[{"award-number":["075-15-2025-487"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]},{"award":["075-15-2025-487"],"award-info":[{"award-number":["075-15-2025-487"]}],"id":[{"id":"https:\/\/ror.org\/00ghqgy32","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: Acomys cahirinus exhibits more favorable cardiac remodeling after ischemic injury than Mus musculus, yet the molecular organization of the intact myocardium remains poorly characterized. This study aimed to identify baseline interspecies differences in the adult left ventricle, with particular emphasis on ventricular cardiomyocyte transcriptional states. Methods: Intact left ventricles from adult male A. cahirinus and M. musculus were analyzed by single-nucleus RNA sequencing using three biological samples per species. Cross-species integration was followed by cell type annotation, pseudobulk differential expression analysis, Augur-based assessment of interspecies separability, coexpression module analysis, and focused reintegration analysis of ventricular cardiomyocytes. Cardiomyocyte profiles were further compared with published embryonic and early postnatal M. musculus heart datasets. Results: Both species contained comparable major myocardial cell populations, whereas marked interspecies transcriptional differences persisted within individual cell types. Ventricular cardiomyocytes exhibited the largest number of differentially expressed genes and were classified into three transcriptional states: contractile, matrix-associated, and alternative. Adult A. cahirinus cardiomyocytes retained a mature cardiomyocyte phenotype but showed greater enrichment of developmental, morphogenetic, and Hippo-associated gene programs than adult M. musculus cardiomyocytes. Their transcriptional profile did not correspond to embryonic or early postnatal mouse states. The integrated atlas further identified interspecies differences in fibroblast-associated and extracellular matrix-related programs. Conclusions: The intact myocardium of A. cahirinus exhibits cell type-specific transcriptional differences relative to M. musculus. Ventricular cardiomyocytes display a distinct mature transcriptional configuration rather than a direct immature state, which may contribute to the favorable response of A. cahirinus to myocardial injury.<\/jats:p>","DOI":"10.3390\/genes17091071","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:07:32Z","timestamp":1788538052000},"page":"1071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Single-Nucleus Transcriptomic Analysis of the Intact Left Ventricle in Acomys cahirinus and Mus musculus"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9179-515X","authenticated-orcid":false,"given":"Nikita","family":"Filatov","sequence":"first","affiliation":[{"name":"National Medical Research Centre of Cardiology Named After Academician E.I. Chazov, Ministry of Health of the Russian Federation, Moscow 121552, Russia"},{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0331-8765","authenticated-orcid":false,"given":"Kirill","family":"Kokorichev","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Cardiology Named After Academician E.I. Chazov, Ministry of Health of the Russian Federation, Moscow 121552, Russia"},{"name":"Institution \u201cCentre for Strategic Planning and Management of Biomedical Health Risks\u201d, Federal Medical Biological Agency, Centre for Strategic Planning of the FMBA of Russia, 10-1 Pogodinskaya Street, Moscow 119121, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2032-537X","authenticated-orcid":false,"given":"Dmitry","family":"Tychinin","sequence":"additional","affiliation":[{"name":"Institution \u201cCentre for Strategic Planning and Management of Biomedical Health Risks\u201d, Federal Medical Biological Agency, Centre for Strategic Planning of the FMBA of Russia, 10-1 Pogodinskaya Street, Moscow 119121, Russia"},{"name":"Life Improvement by Future Technologies (LIFT) Center, Moscow 121205, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-7150","authenticated-orcid":false,"given":"Taisiya","family":"Voronina","sequence":"additional","affiliation":[{"name":"Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420008, Russia"},{"name":"Life Improvement by Future Technologies (LIFT) Center, Moscow 121205, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2339-261X","authenticated-orcid":false,"given":"Elena","family":"Shagimardanova","sequence":"additional","affiliation":[{"name":"Life Improvement by Future Technologies (LIFT) Center, Moscow 121205, Russia"},{"name":"Genomics and Bioimaging Core Facility, Skolkovo Institute of Science and Technology, Moscow 143026, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Konstantin","family":"Dergilev","sequence":"additional","affiliation":[{"name":"National Medical Research Centre of Cardiology Named After Academician E.I. 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