{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:04:00Z","timestamp":1789981440392,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["252300420726"],"award-info":[{"award-number":["252300420726"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570544"],"award-info":[{"award-number":["32570544"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32570544"],"award-info":[{"award-number":["32570544"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Investigation of Aquatic Biodiversity and Environmental Conditions in Key Waters of Henan Province"},{"name":"Aquatic Seed Industry Research Project in Henan Province"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Background\/Objectives: The monotypic genus Araiocypris is currently classified in Xenocyprididae, yet its familial placement has long been controversial and lacks sufficient molecular phylogenetic support. Mitogenomic resources for the family Tanichthyidae remain limited, and the phylogenetic relationship between Araiocypris and tanichthyid species remains unexplored. This study was conducted to re-evaluate the taxonomic status of Araiocypris and characterize the mitogenomic evolution of Tanichthyidae. Methods: The complete mitochondrial genomes of Tanichthys huidongensis and Araiocypris batodes were sequenced, assembled and annotated. Comparative mitogenomic analyses were performed across seven Tanichthyidae species. A maximum-likelihood phylogenetic tree was reconstructed using concatenated sequences of 13 protein-coding genes and 2 ribosomal RNA genes to clarify the phylogenetic position of Araiocypris. Results: Based on mitochondrial phylogenomic evidence, phylogenetic reconstruction strongly supports the reassignment of A. batodes from Xenocyprididae to Tanichthyidae, as it nests within the Tanichthyidae clade and forms a sister group with the genus Tanichthys. Genetic divergence analysis confirms the validity of Araiocypris as a distinct genus. Tanichthyidae mitogenomes display highly conserved architecture with the typical vertebrate gene order. All protein-coding genes undergo strong purifying selection, with cox1 showing the slowest evolutionary rate. Conserved structural domains including CSB-F, CSB-E, CSB-D and CSB1\u20133 were identified in the control region. Conclusions: This study provides solid mitogenomic evidence for the familial reclassification of Araiocypris and systematically reveals the evolutionary patterns of Tanichthyidae mitogenomes, offering an important reference for further taxonomic and phylogenetic studies of Cypriniformes.<\/jats:p>","DOI":"10.3390\/genes17091153","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:03:47Z","timestamp":1789977827000},"page":"1153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Complete Mitochondrial Genomes of Araiocypris batodes and Tanichthys huidongensis Provide Phylogenetic Evidence for the Reassignment of Araiocypris to Tanichthyidae"],"prefix":"10.3390","volume":"17","author":[{"given":"Liangjie","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Chunhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Zunyi Vocational and Technical College, Zunyi 563000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Shufang","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0765-0351","authenticated-orcid":false,"given":"Junjie","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science, South China Normal University, Guangzhou 510631, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Chaoqun","family":"Su","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7100-1989","authenticated-orcid":false,"given":"Chenxi","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Jiahui","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Gaoyou","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Xusheng","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1909-3610","authenticated-orcid":false,"given":"Shaoyong","family":"Jing","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Tiezhu","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China"},{"name":"Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Fan","family":"Li","sequence":"additional","affiliation":[{"name":"Branch of Shanghai Science and Technology Museum, Shanghai Natural History Museum, Shanghai 200041, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"ref_1","first-page":"424","article-title":"Inferring the Tree of Life of the Order Cypriniformes, the Earth\u2019s Most Diverse Clade of Freshwater Fishes: Implications of Varied Taxon and Character Sampling","volume":"46","author":"Mayden","year":"2008","journal-title":"J. Syst. Evol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1093\/oxfordjournals.molbev.a003741","article-title":"Mitogenomic Exploration of Higher Teleostean Phylogenies: A Case Study for Moderate-Scale Evolutionary Genomics with 38 Newly Determined Complete Mitochondrial DNA Sequences","volume":"18","author":"Miya","year":"2001","journal-title":"Mol. Biol. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.ympev.2008.12.015","article-title":"Reconstructing the Phylogenetic Relationships of the Earth\u2019s Most Diverse Clade of Freshwater Fishes\u2014Order Cypriniformes (Actinopterygii: Ostariophysi): A Case Study Using Multiple Nuclear Loci and the Mitochondrial Genome","volume":"51","author":"Mayden","year":"2009","journal-title":"Mol. Phylogenetics Evol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.ympev.2009.01.006","article-title":"Molecular Systematics of the Cyprinoidea (Teleostei: Cypriniformes), the World\u2019s Largest Clade of Freshwater Fishes: Further Evidence from Six Nuclear Genes","volume":"52","author":"Chen","year":"2009","journal-title":"Mol. Phylogenetics Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"21067.1-13","DOI":"10.25225\/jvb.21067","article-title":"Two New Species of Tanichthys (Teleostei: Cypriniformes) from China","volume":"71","author":"Li","year":"2022","journal-title":"J. Vertebr. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1111\/jfb.15012","article-title":"Descriptions of Six New Species in White Cloud Mountain Minnow Tanichthys albonubes Complex (Cypriniformes: Tanichthyidae) in Southern China","volume":"100","author":"Jin","year":"2022","journal-title":"J. Fish. Biol."},{"key":"ref_7","unstructured":"Fricke, R., Eschmeyer, W.N., and van der Laan, R. (2026). Eschmeyer\u2019s Catalog of Fishes: Genera, Species, References, California Academy of Sciences."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1080\/23802359.2023.2282794","article-title":"The Complete Mitochondrial Genome of the Tanichthys flavianalis (Cypriniformes: Tanichthyidae) and Its Phylogeny","volume":"8","author":"Gong","year":"2023","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/23802359.2023.2171244","article-title":"Characterization of the Complete Mitochondrial Genome Sequence of Tanichthys albiventris and Phylogenetic Analysis","volume":"8","author":"Yang","year":"2023","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_10","first-page":"101","article-title":"Araiocypris Batodes, a New Genus and Species of Cyprinid Fish from Northern Vietnam (Ostariophysi: Cyprinidae)","volume":"56","author":"Conway","year":"2008","journal-title":"Raffles Bull. Zool."},{"key":"ref_11","unstructured":"Li, F. (2024). Freshwater Fishes of China, Straits Publishing House."},{"key":"ref_12","unstructured":"Eschmeyer, W.N., Fricke, R., and van der Laan, R. (2016). Catalog of Fishes: Genera, Species, References, California Academy of Sciences."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6","DOI":"10.11646\/zootaxa.4476.1.4","article-title":"Phylogenetic Classification of Extant Genera of Fishes of the Order Cypriniformes (Teleostei: Ostariophysi)","volume":"4476","author":"Tan","year":"2018","journal-title":"Zootaxa"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Stout, C.C., Tan, M., Lemmon, A.R., Lemmon, E.M., and Armbruster, J.W. (2016). Resolving Cypriniformes Relationships Using an Anchored Enrichment Approach. BMC Evol. Biol., 16.","DOI":"10.1186\/s12862-016-0819-5"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1385\/1-59259-192-2:71","article-title":"DNASTAR\u2019s Lasergene Sequence Analysis Software","volume":"Volume 132","author":"Misener","year":"1999","journal-title":"Bioinformatics Methods and Protocols"},{"key":"ref_16","first-page":"95","article-title":"BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT","volume":"Volume 41","author":"Hall","year":"1999","journal-title":"Proceedings of the Nucleic Acids Symposium Series"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1093\/molbev\/mst141","article-title":"MitoFish and MitoAnnotator: A Mitochondrial Genome Database of Fish with an Accurate and Automatic Annotation Pipeline","volume":"30","author":"Iwasaki","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2582","DOI":"10.1093\/molbev\/msy159","article-title":"MACSE v2: Toolkit for the Alignment of Coding Sequences Accounting for Frameshifts and Stop Codons","volume":"35","author":"Ranwez","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1111\/1755-0998.13096","article-title":"PhyloSuite: An Integrated and Scalable Desktop Platform for Streamlined Molecular Sequence Data Management and Evolutionary Phylogenetics Studies","volume":"20","author":"Zhang","year":"2020","journal-title":"Mol. Ecol. Resour."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: Fast Model Selection for Accurate Phylogenetic Estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"W242","DOI":"10.1093\/nar\/gkw290","article-title":"Interactive Tree of Life (iTOL) v3: An Online Tool for the Display and Annotation of Phylogenetic and Other Trees","volume":"44","author":"Letunic","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-Depth Characterization and Visualization of Bacterial Genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.gpb.2021.12.002","article-title":"KaKs_Calculator 3.0: Calculating Selective Pressure on Coding and Non-Coding Sequences","volume":"20","author":"Zhang","year":"2022","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9077","DOI":"10.1093\/nar\/gkab688","article-title":"tRNAscan-SE 2.0: Improved Detection and Functional Classification of Transfer RNA Genes","volume":"49","author":"Chan","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Alvanou, M.V., Apostolidis, A.P., Lattos, A., Michaelidis, B., and Giantsis, I.A. (2023). The Coding Mitogenome of the Freshwater Crayfish Pontastacus leptodactylus (Decapoda:Astacidea:Astacidae) from Lake Vegoritida, Greece and Its Taxonomic Classification. Genes, 14.","DOI":"10.3390\/genes14020494"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1002\/aqc.2840","article-title":"Genetic Diversity and Population History of Tanichthys albonubes (Teleostei: Cyprinidae): Implications for Conservation","volume":"28","author":"Zhao","year":"2018","journal-title":"Aquat. Conserv. Mar. Freshw. Ecosyst."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"474","DOI":"10.17520\/biods.2019290","article-title":"Genetic relationships of hatchery populations and wild populations of Tanichthys albonubes near Guangzhou","volume":"28","author":"Li","year":"2020","journal-title":"Biodivers. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1046\/j.1365-294X.2003.02063.x","article-title":"The Incomplete Natural History of Mitochondria","volume":"13","author":"Ballard","year":"2004","journal-title":"Mol. Ecol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3109\/19401736.2013.869682","article-title":"Complete Sequence and Rearrangement of the Mitochondrial Genome of Garra qiaojiensis (Cypriniformes: Cyprinidae)","volume":"27","author":"Xiong","year":"2016","journal-title":"Mitochondrial DNA Part A"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhang, C., Zhang, K., Peng, Y., Zhou, J., Liu, Y., and Liu, B. (2022). Novel Gene Rearrangement in the Mitochondrial Genome of Three Garra and Insights Into the Phylogenetic Relationships of Labeoninae. Front. Genet., 13.","DOI":"10.3389\/fgene.2022.922634"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1080\/23802359.2023.2290128","article-title":"Complete Mitochondrial Genome of Golden Variant of Freshwater Fish Labeo rajasthanicus (Cypriniformes: Cyprinidae): Endemic to India","volume":"8","author":"Singh","year":"2023","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1080\/23802359.2023.2288437","article-title":"The Complete Mitochondrial Genome of a New Species of the Genus Schizothorax from Sichuan, China (Cypriniformes: Cyprinidae)","volume":"8","author":"Wang","year":"2023","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1080\/23802359.2024.2318401","article-title":"The Complete Mitochondrial Genome of Gobio Huanghensis (Cypriniformes: Cyprinidae) in the Yellow River, China","volume":"9","author":"Du","year":"2024","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1080\/23802359.2023.2274617","article-title":"The Complete Mitochondrial Genome of Rhinogobius maculagenys (Gobiidae:Gobionellinae)","volume":"8","author":"Hu","year":"2023","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1080\/23802359.2024.2410438","article-title":"Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of the Hybrid Flat Fish Platichthys stellatus (\u2640) \u00d7 Platichthys bicoloratus (\u2642)","volume":"9","author":"Baek","year":"2024","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/S0168-9525(02)02722-1","article-title":"The Ka\/Ks Ratio: Diagnosing the Form of Sequence Evolution","volume":"18","author":"Hurst","year":"2002","journal-title":"Trends Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1745-6150-6-13","article-title":"Nonsynonymous Substitution Rate (Ka) Is a Relatively Consistent Parameter for Defining Fast-Evolving and Slow-Evolving Protein-Coding Genes","volume":"6","author":"Wang","year":"2011","journal-title":"Biol. Direct"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"7819","DOI":"10.1002\/ece3.5369","article-title":"The Complete Mitochondrial Genome of Rhynchocypris oxycephalus (Teleostei: Cyprinidae) and Its Phylogenetic Implications","volume":"9","author":"Zhang","year":"2019","journal-title":"Ecol. Evol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Liu, C., Li, D., Zhang, Y., P\u00e9r\u00e9, M., Zhuang, Z., Liu, J., Zhou, H., and Chen, X. (2023). Phylogenetic Analyses of Pristipomoides (Perciformes: Lutjanidae) Based on New Mitochondrial Genomes. Fishes, 8.","DOI":"10.3390\/fishes8070343"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Fiteha, Y.G., and Magdy, M. (2022). The Evolutionary Dynamics of the Mitochondrial tRNA in the Cichlid Fish Family. Biology, 11.","DOI":"10.3390\/biology11101522"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1073\/pnas.84.5.1324","article-title":"Bizarre tRNAs Inferred from DNA Sequences of Mitochondrial Genomes of Nematode Worms","volume":"84","author":"Wolstenholme","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zapelloni, F., Jurado-Rivera, J.A., Jaume, D., Juan, C., and Pons, J. (2021). Comparative Mitogenomics in Hyalella (Amphipoda: Crustacea). Genes, 12.","DOI":"10.3390\/genes12020292"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4518","DOI":"10.3390\/ijms16034518","article-title":"tRNA Biology in Mitochondria","volume":"16","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.ympev.2005.08.007","article-title":"Comparison of Evolutionary Rates in the Mitochondrial DNA Cytochrome b Gene and Control Region and Their Implications for Phylogeny of the Cobitoidea (Teleostei: Cypriniformes)","volume":"39","author":"Tang","year":"2006","journal-title":"Mol. Phylogenetics Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1007\/s11033-008-9271-y","article-title":"The Complete Mitochondrial Genome of Rock Carp Procypris rabaudi (Cypriniformes: Cyprinidae) and Phylogenetic Implications","volume":"36","author":"Zhang","year":"2009","journal-title":"Mol. Biol. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.3109\/19401736.2014.958727","article-title":"Complete Mitochondrial Genome of Ptychobarbus kaznakovi (Teleostei: Cypriniformes: Cyprinidae), and Repetitive Sequences in the D-Loop","volume":"27","author":"Ma","year":"2016","journal-title":"Mitochondrial DNA Part A"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1080\/09168451.2016.1270739","article-title":"Comparative Analysis of the Complete Mitochondrial Genomes of Three Geographical Topmouth Culter (Culter alburnus) Groups and Implications for Their Phylogenetics","volume":"81","author":"Shi","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_51","first-page":"2965","article-title":"Mitochondrial Genome Sequence Characteristics and Phylogenetic Analysis of Schizothorax argentatus","volume":"39","author":"Liu","year":"2023","journal-title":"CJBT"},{"key":"ref_52","first-page":"46","article-title":"Structure Analysis and Comparative Study of mtDNA Control Region in Mystus macropterus","volume":"37","author":"Wei","year":"2015","journal-title":"J. Southwest Univ. (Nat. Sci. Ed.)"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3897\/zookeys.546.5964","article-title":"Complete Mitochondrial DNA Sequence of the Endangered Fish (Bahaba Taipingensis): Mitogenome Characterization and Phylogenetic Implications","volume":"546","author":"Zhao","year":"2015","journal-title":"Zookeys"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"146847","DOI":"10.1016\/j.gene.2022.146847","article-title":"The Complete Mitochondrial Genome of the Roosterfish Nematistius pectoralis Gill 1862: Purifying Selection in Protein Coding Genes, Organization of the Control Region, and Insights into Family-Level Phylogenomic Relationships in the Recently Erected Order Carangiformes","volume":"845","author":"Baeza","year":"2022","journal-title":"Gene"},{"key":"ref_55","first-page":"266","article-title":"Structure and evolution of mitochondrial DNA control region in fishs: A case study to bitterlings fishes","volume":"12","author":"Liu","year":"2002","journal-title":"Prog. Nat. Sci."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/9\/1153\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:26:10Z","timestamp":1789979170000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/17\/9\/1153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,20]]},"references-count":55,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["genes17091153"],"URL":"https:\/\/doi.org\/10.3390\/genes17091153","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,20]]}}}