{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T21:13:48Z","timestamp":1776287628397,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T00:00:00Z","timestamp":1510272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Virginia Bioinformatics Institute Core Lab and Fralin Life Science Institute Next-Gen DNA sequencing grant"},{"name":"Virginia Agricultural Experiment Station"},{"name":"Molecular\/Translational Plant Science graduate student research and travel awards"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>Contact with poison ivy plants is widely dreaded because they produce a natural product called urushiol that is responsible for allergenic contact delayed-dermatitis symptoms lasting for weeks. For this reason, the catchphrase most associated with poison ivy is \u201cleaves of three, let it be\u201d, which serves the purpose of both identification and an appeal for avoidance. Ironically, despite this notoriety, there is a dearth of specific knowledge about nearly all other aspects of poison ivy physiology and ecology. As a means of gaining a more molecular-oriented understanding of poison ivy physiology and ecology, Next Generation DNA sequencing technology was used to develop poison ivy root and leaf RNA-seq transcriptome resources. De novo assembled transcriptomes were analyzed to generate a core set of high quality expressed transcripts present in poison ivy tissue. The predicted protein sequences were evaluated for similarity to SwissProt homologs and InterProScan domains, as well as assigned both GO terms and KEGG annotations. Over 23,000 simple sequence repeats were identified in the transcriptome, and corresponding oligo nucleotide primer pairs were designed. A pan-transcriptome analysis of existing Anacardiaceae transcriptomes revealed conserved and unique transcripts among these species.<\/jats:p>","DOI":"10.3390\/genes8110317","type":"journal-article","created":{"date-parts":[[2017,11,10]],"date-time":"2017-11-10T11:12:26Z","timestamp":1510312346000},"page":"317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Sequencing and De Novo Assembly of the Toxicodendron radicans (Poison Ivy) Transcriptome"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0045-1368","authenticated-orcid":false,"given":"Alexandra","family":"Weisberg","sequence":"first","affiliation":[{"name":"Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97330, USA"},{"name":"Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunjune","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5127-7294","authenticated-orcid":false,"given":"James","family":"Westwood","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7088-8020","authenticated-orcid":false,"given":"John","family":"Jelesko","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1016\/S0196-0644(87)80739-4","article-title":"Plant-induced dermatitis","volume":"16","author":"Epstein","year":"1987","journal-title":"Ann. Emerg. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0733-8635(18)30155-4","article-title":"Occupational poison ivy and oak dermatitis","volume":"3","author":"Epstein","year":"1994","journal-title":"Derrmatol. Clin."},{"key":"ref_3","first-page":"99","article-title":"Toxicodendron dermatitis","volume":"42","author":"Gayer","year":"1988","journal-title":"Cutis"},{"key":"ref_4","unstructured":"Mitchell, J., and Rook, A. (1979). Botanical Dermatology: Plants and Plant Products Injurious to the Skin, Greengrass."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.1021\/ja01327a064","article-title":"The toxic principle of the poison ivy","volume":"56","author":"Hill","year":"1934","journal-title":"J. Am. Chem. Soc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/cber.19220550123","article-title":"Uber den hauptbestandteil des japan-lacs, viii. Mitteilung: Stellung der doppelbindungen in der seitenkette des urushiols und beweisfuhrung, dab das urushiol eine mischung ist","volume":"55","author":"Majima","year":"1922","journal-title":"Ber. Dtsch. Chem. Ges."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1002\/cber.19070400472","article-title":"Ueber einen hauptbestandteil des japanischen lackes","volume":"40","author":"Majima","year":"1907","journal-title":"Ber. Dtsch. Chem. Ges."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1021\/jo01016a067","article-title":"On the isolation of the allegenically active components of the toxic principle of poison ivy","volume":"30","author":"Markiewitz","year":"1965","journal-title":"J. Org. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/171841b0","article-title":"Separation and structural determination of the olefinic components of poison ivy urushiol, cardanol and cardol","volume":"171","author":"Symes","year":"1953","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/jps.2600670411","article-title":"New GLC analysis of urushiol congeners in different plant parts of poison ivy, Toxicodendron radicans","volume":"67","author":"Craig","year":"1978","journal-title":"J. Pharmacol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Aziz, M., Sturtevant, D., Winston, J., Collakova, E., Jelesko, J.G., and Chapman, K.D. (2017). MALDI-MS imaging of urushiols in poison ivy stem. Molecules, 22.","DOI":"10.3390\/molecules22050711"},{"key":"ref_12","first-page":"161","article-title":"The systematics and ecology of poison-ivy and the poison-oaks (Toxicodendron, Anacardiaceae)","volume":"73","author":"Gillis","year":"1971","journal-title":"Rhodora"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"616","DOI":"10.5962\/p.358663","article-title":"The dispersal of fruits and seeds of poison-ivy, Toxicodendron radicans, by ruffed grouse, Bonasa umbellus, and squirrels, Tamiasciurus hudsonicus and Sciurus carolinensis","volume":"113","author":"Penner","year":"1999","journal-title":"Can. Field-Nat."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.2307\/2265596","article-title":"Host preferences of Rhus radicans (Anacardiaceae) in a southern deciduous harwood forest","volume":"77","author":"Talley","year":"1996","journal-title":"Ecology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9086","DOI":"10.1073\/pnas.0602392103","article-title":"Biomass and toxicity responses of poison ivy (Toxicodendron radicans) to elevated atmospheric CO2","volume":"103","author":"Mohan","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1614\/WS-06-190","article-title":"Rising atmospheric carbon dioxide and potential impacts on the growth and toxicity of poison ivy (Toxicodendron radicans)","volume":"55","author":"Ziska","year":"2007","journal-title":"Weed Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"93","DOI":"10.5962\/p.267783","article-title":"Poison ivy and its kin","volume":"35","author":"Gillis","year":"1975","journal-title":"Arnoldia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"34","DOI":"10.2307\/2996596","article-title":"Hurricane impacts on liana populations in an old-growth southeastern bottomland forest","volume":"124","author":"Allen","year":"1997","journal-title":"J. Torrey Bot. Soc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.foreco.2005.08.021","article-title":"Twelve years post-hurricane liana dynamics in an old-growth southeastern floodplain forest","volume":"218","author":"Allen","year":"2005","journal-title":"For. Ecol. Manag."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"571","DOI":"10.5962\/p.363508","article-title":"Plant community composition and relationships of disturbed and undisturbed alvar woodland","volume":"116","author":"Catling","year":"2002","journal-title":"Can. Field-Nat."},{"key":"ref_21","unstructured":"Martin, A.C., Zim, H.S., and Nelson, A.L. (1951). American Wildlife & Plants, Dover Publications, Inc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1017\/S0890037X00045942","article-title":"Grazing animals as weed control agents","volume":"10","author":"Popay","year":"1996","journal-title":"Weed Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ppees.2008.02.001","article-title":"Fungal and animal associates of Toxicodendron spp. (Anacardiaceae) in North America","volume":"10","author":"Senchina","year":"2008","journal-title":"Perspect. Plant Ecol. Evol. Syst."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1676\/0043-5643(2000)112[0249:UOSHAF]2.0.CO;2","article-title":"Use of successional habitat and fruit resources by songbirds during autumn migration in central New Jersey","volume":"112","author":"Suthers","year":"2000","journal-title":"Wilson Bull."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1649\/790.1","article-title":"Beetle interactions with poison ivy and poison oak (Toxicodendron p. Mill. Sect. Toxicodendron, Anacardiaceae)","volume":"59","author":"Senchina","year":"2005","journal-title":"Coleopt. Bull."},{"key":"ref_26","first-page":"120","article-title":"Great diversity of insect floral associates may partially explain ecological success of poison ivy (Toxicodendron radicans subps negundo greene gillis, Anacardiaceae)","volume":"40","author":"Senchina","year":"2007","journal-title":"Gt. Lakes Entomol."},{"key":"ref_27","unstructured":"Dewick, P.M. (1997). Medicinal Natural Products: A Biosynthetic Approach, John Wiley & Sons."},{"key":"ref_28","unstructured":"Bernfeld, P. (1967). The biosynthesis of phenolic plant products. The Biogenesis of Natural Compounds, Pergamon Press Ltd.. [2nd ed.]."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1656\/045.024.0210","article-title":"Differential responses to light and nutrient availability by geographically isolated poison ivy accessions","volume":"24","author":"Jelesko","year":"2017","journal-title":"Northeast. Nat."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0091-6749(72)90120-0","article-title":"Comparison of the contact allergenicity of the four pentadecylcatechols derived from poison ivy urushiol in human subjects","volume":"49","author":"Johnson","year":"1972","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21273\/HORTSCI.48.12.1525","article-title":"Germinating and culturing axenic poison ivy seedlings","volume":"48","author":"Benhase","year":"2013","journal-title":"HortScience"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","article-title":"A revised medium for rapid growth and bio assays with tobacco tissue cultures","volume":"15","author":"Murashige","year":"1962","journal-title":"Physiol. Plant."},{"key":"ref_33","unstructured":"Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (2006). Current Protocols in Molecular Biology, John Wiley & Sons."},{"key":"ref_34","unstructured":"Andrews, S. (2010, April 26). FastQC: A Quality Control Tool for High Throughput Sequence Data. Available online: http:\/\/www.Bioinformatics.Babraham.Ac.Uk\/projects\/fastqc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"237","DOI":"10.3389\/fgene.2013.00237","article-title":"Blobology: Exploring raw genome data for contaminants, symbionts and parasites using taxon-annotated GC-coverage plots","volume":"4","author":"Kumar","year":"2013","journal-title":"Front. Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3210","DOI":"10.1093\/bioinformatics\/btv351","article-title":"BUSCO: Assessing genome assembly and annotation completeness with single-copy orthologs","volume":"31","author":"Simao","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-Seq using the Trinity platform for reference generation and analysis","volume":"8","author":"Haas","year":"2013","journal-title":"Nat. Protocols"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W29","DOI":"10.1093\/nar\/gkr367","article-title":"Hmmer web server: Interactive sequence similarity searching","volume":"39","author":"Finn","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"D222","DOI":"10.1093\/nar\/gkt1223","article-title":"Pfam: The protein families database","volume":"42","author":"Finn","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nmeth.1701","article-title":"Signalp 4.0: Discriminating signal peptides from transmembrane regions","volume":"8","author":"Petersen","year":"2011","journal-title":"Nat. Methods"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1093\/nar\/gkm160","article-title":"Rnammer: Consistent and rapid annotation of ribosomalRNA genes","volume":"35","author":"Lagesen","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The sequence alignment\/map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C. (2011). RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"R14","DOI":"10.1186\/gb-2010-11-2-r14","article-title":"Gene ontology analysis for RNA-seq: Accounting for selection bias","volume":"11","author":"Young","year":"2010","journal-title":"Genome Biol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Foster, Z., Sharpton, T., and Grunwald, N. (2017). Metacoder: An R package for visualization and manipulation of community taxonomic diversity data. PLoS Comp. Biol., 13.","DOI":"10.1371\/journal.pcbi.1005404"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkl031","article-title":"WEGO: A web tool for plotting GO annotations","volume":"34","author":"Ye","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_52","unstructured":"Thiel, T. (2017, July 31). Misa\u2014Microsatellite identification tool. Available online: http:\/\/pgrc.ipk-gatersleben.de\/misa\/."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3--new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_54","first-page":"184","article-title":"Analysis of plant pan-genomes and transcriptomes with GET_HOMOLOGUES-EST, a clustering solution for sequences of the same species","volume":"8","author":"Cantalapiedra","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Backman, T.W.H., and Girke, T. (2016). SystemPipeR: NGS workflow and report generation environment. BMC Bioinform., 17.","DOI":"10.1186\/s12859-016-1241-0"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/j.1759-6831.2009.00045.x","article-title":"Phylogenetic analysis of Toxicodendron (Anacardiaceae) and its biogeographic implications on the evolution of north temperate and tropical intercontinental disjunctions","volume":"47","author":"Nie","year":"2009","journal-title":"J. Syst. Evol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lulin, H., Xiao, Y., Pei, S., Wen, T., and Shangqin, H. (2012). The first Illumina-based de novo transcriptome sequencing and analysis of safflower flowers. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0038653"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1104\/pp.113.234864","article-title":"De novo assembly and characterization of the transcriptome of the parasitic weed dodder identifies genes associated with plant parasitism","volume":"166","author":"Ranjan","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Zhang, J., Ruhlman, T., Mower, J., and Jansen, R. (2013). Comparative analyses of two Geraniaceae transcriptomes using next-generation sequencing. BMC Plant Biol., 13.","DOI":"10.1186\/1471-2229-13-228"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Kalra, S., Puniya, B., Kulshreshtha, D., Kumar, S., Kaur, J., Ramachandran, S., and Singh, K. (2013). De novo transcriptome sequencing reveals important molecular networks and metabolic pathways of the plant, Chlorophytum borivilianum. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0083336"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Liu, S., Li, W., Wu, Y., Chen, C., and Lei, J. (2013). De novo transcriptome assembly in chili pepper (Capsicum frutescens) to identify genes involved in the biosynthesis of capsaicinoids. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0048156"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1093\/bioinformatics\/bts094","article-title":"Oases: Robust de novoRNA-Seq assembly across the dynamic range of expression levels","volume":"28","author":"Schulz","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1093\/bioinformatics\/btu077","article-title":"SOAPdenovo-Trans: De novo transcriptome assembly with short RNA-Seq reads","volume":"30","author":"Xie","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1094\/PDIS-09-13-0946-PDN","article-title":"First report of seedling blight of eastern poison ivy (Toxicodendron radicans) by Colletotrichum fioriniae in Virginia","volume":"98","author":"Kasson","year":"2014","journal-title":"Plant Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3852\/07-174R","article-title":"Colletotrichum acutatum var. Fioriniae (teleomorph: Glomerella acutata var. Fioriniae var. Nov.) infection of a scale insect","volume":"100","author":"Marcelino","year":"2008","journal-title":"Mycologia"},{"key":"ref_66","first-page":"13","article-title":"Entomopathogenic activity of a variety of the fungus, Colletotrichum acutatum, recovered from the elongate hemlock scale, Fiorinia externa","volume":"9","author":"Marcelino","year":"2009","journal-title":"J. Insect Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1663\/0013-0001(2003)057[0354:TPIVAS]2.0.CO;2","article-title":"Toxic phenols in various Anacardiaceae species","volume":"57","author":"Sosa","year":"2003","journal-title":"Econ. Bot."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"20414","DOI":"10.3390\/ijms141020414","article-title":"Characterization of 42 microsatellite markers from poison ivy, Toxicodendron radicans (Anacardiaceae)","volume":"14","author":"Hsu","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"265","DOI":"10.2307\/2394183","article-title":"A monographic study of Rhus and its immediate allies in North and Central America, including the West Indies","volume":"37","author":"Barkley","year":"1937","journal-title":"Ann. MO. Bot. Gard."}],"container-title":["Genes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4425\/8\/11\/317\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:48:51Z","timestamp":1760208531000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4425\/8\/11\/317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,10]]},"references-count":69,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11]]}},"alternative-id":["genes8110317"],"URL":"https:\/\/doi.org\/10.3390\/genes8110317","relation":{},"ISSN":["2073-4425"],"issn-type":[{"value":"2073-4425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,10]]}}}