{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:45:20Z","timestamp":1774323920246,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:00:00Z","timestamp":1540771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.31572592 and No.31472256"],"award-info":[{"award-number":["No.31572592 and No.31472256"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Key Laboratory Innovation Platform Fund of Bureau of Hunan Province","award":["15K011"],"award-info":[{"award-number":["15K011"]}]},{"name":"the Science and Technology Project of Changsha","award":["K1705044 and ZD1601003"],"award-info":[{"award-number":["K1705044 and ZD1601003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes"],"abstract":"<jats:p>The peripheral tissue pacemaker is responsive to light and other zeitgebers, especially food availability. Generally, the pacemaker can be reset and entrained independently of the central circadian structures. Studies involving clock-gene expressional patterns in fish peripheral tissues have attracted considerable attention. However, the rhythmic expression of clock genes in skeletal muscle has only scarcely been investigated. The present study was designed to investigate the core clock and functional gene expression rhythms in crucian carp. Meanwhile, the synchronized effect of food restrictions (short-term fasting) on these rhythms in skeletal muscle was carefully examined. In fed crucian carp, three core clock genes (Clock, Bmal1a, and Per1) and five functional genes (Epo, Fas, IGF1R2, Jnk1, and MyoG) showed circadian rhythms. By comparison, four core clock genes (Clock, Bmal1a, Cry3, and Per2) and six functional genes (Epo, GH, IGF2, Mstn, Pnp5a, and Ucp1) showed circadian rhythms in crucian carp muscle after 7-day fasting. In addition, three core clock genes (Clock, Per1, and Per3) and six functional genes (Ampk1a, Lpl, MyoG, Pnp5a, PPAR\u03b1, and Ucp1) showed circadian rhythms in crucian carp muscle after 15-day fasting. However, all gene rhythmic expression patterns differed from each other. Furthermore, it was found that the circadian genes could be altered by feed deprivation in crucian carp muscle through the rhythms correlation analysis of the circadian genes and functional genes. Hence, food-anticipatory activity of fish could be adjusted through the food delivery restriction under a light\u2013dark cycle. These results provide a potential application in promoting fish growth by adjusting feeding conditions and nutritional state.<\/jats:p>","DOI":"10.3390\/genes9110526","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T11:10:41Z","timestamp":1540811441000},"page":"526","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Impact of Short-Term Fasting on The Rhythmic Expression of the Core Circadian Clock and Clock-Controlled Genes in Skeletal Muscle of Crucian Carp (Carassius auratus)"],"prefix":"10.3390","volume":"9","author":[{"given":"Ping","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China"},{"name":"Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingsheng","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Aquatic Biotechnology, University of Duisburg-Essen, 45141 Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute of Hunan Province, Changsha 410153, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanan","family":"Wu","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute of Hunan Province, Changsha 410153, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianshe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"He","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute of Hunan Province, Changsha 410153, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuying","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Engineering, Changsha University, Changsha 410003, China"},{"name":"Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.conb.2013.02.018","article-title":"Circadian timekeeping and output mechanisms in animals","volume":"23","author":"Hardin","year":"2013","journal-title":"Curr. 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