{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:50:09Z","timestamp":1782136209773,"version":"3.54.5"},"reference-count":44,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Disease Reports"],"abstract":"<jats:p>Infections represent an important problem in neonates because of the high mortality. An increase in neonatal infections has been found in Cuban hospitals in recent years. The aim of this study was to provide evidence on the clinical and microbiological behavior of Gram-negative bacilli that cause neonatal infections in hospitals of Havana, Cuba. It was carried out as a descriptive cross-sectional investigation from September 2017 to July 2018 in The Tropical Medicine Institute \u201cPedro Kouri\u201d (IPK). Sixty-one Gram-negative bacilli isolated from neonates with infections in six Gyneco-Obstetric and Pediatric Hospitals of Havana were analyzed for their species and antimicrobial susceptibility. Late-onset infections were more common than early-onset ones and included urinary tract infection in the community (87%) and sepsis in hospitals (63.3%). Catheter use (47%) and prolonged stay (38%) were the most frequent risk factors. Species of major pathogens were Escherichia coli (47%) and Klebsiella spp. (26%). The isolated Gram-negative bacilli showed high resistance rates to third-generation cephalosporins, ciprofloxacin and gentamicin, while being more susceptible to carbapenems, fosfomycin, colistin and amikacin. The present study revealed the clinical impact of Gram-negative bacilli in neonatology units in hospitals of Havana. Evaluation of antimicrobial susceptibilities to the isolates from neonates is necessary for selection of appropriate empirical therapy and promotion of the rational antibiotic use.<\/jats:p>","DOI":"10.3390\/idr13010025","type":"journal-article","created":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T06:22:32Z","timestamp":1615270952000},"page":"219-229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Characterization of Neonatal Infections by Gram-Negative Bacilli and Associated Risk Factors, Havana, Cuba"],"prefix":"10.3390","volume":"13","author":[{"given":"Arlenis","family":"Oliva","sequence":"first","affiliation":[{"name":"William Soler Hospital, Havana 10800, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yenisel","family":"Carmona","sequence":"additional","affiliation":[{"name":"Tropical Medicine Institute \u201cPedro Kouri\u201d, Havana 11400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"de La C. L\u00f3pez","sequence":"additional","affiliation":[{"name":"Public Health Ministry, Havana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Public Health Ministry, Havana 10400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8402-5349","authenticated-orcid":false,"given":"Meiji Soe","family":"Aung","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0146-6211","authenticated-orcid":false,"given":"Nobumichi","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianelys","family":"Qui\u00f1ones","sequence":"additional","affiliation":[{"name":"Tropical Medicine Institute \u201cPedro Kouri\u201d, Havana 11400, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,9]]},"reference":[{"key":"ref_1","first-page":"80","article-title":"Neonatal sepsis etiology and sensitivity to antibiotics in the new Hospital in Guadalajara","volume":"16","author":"Mansilla","year":"2011","journal-title":"Rev. Mex Enferm. Infecc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3109\/00365548.2014.971053","article-title":"Diagnostic utility of biomarkers for neonatal sepsis\u2014A systematic review","volume":"47","author":"Hedegaard","year":"2014","journal-title":"Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s13052-018-0515-8","article-title":"Secondary prevention of early-onset sepsis: A less invasive Italian approach for managing neonates at risk","volume":"44","author":"Berardi","year":"2018","journal-title":"Ital. J. Pediatr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0140-6736(14)60496-7","article-title":"Every Newborn: Progress, priorities, and potential beyond survival","volume":"384","author":"Lawn","year":"2014","journal-title":"Lancet"},{"key":"ref_5","first-page":"228","article-title":"Mortalidad neonatal, an\u00e1lisis de registros de vigilancia e historias cl\u00ednicas neonatales del a\u00f1o 2011 en Hu\u00e1nuco Y Ucayali, Per\u00fa","volume":"31","author":"Kusunoki","year":"2014","journal-title":"Rev. Peru Med. Exp. Salud Publica"},{"key":"ref_6","unstructured":"(2016). Statistical Health Yearbook, Direction of Medical Records and Health Statistics, ISSN Electronic Version."},{"key":"ref_7","first-page":"52","article-title":"Neonatal infection: Behavior in an intensive care unit","volume":"82","author":"Franco","year":"2010","journal-title":"Rev. Cubana Pediatr\u00eda"},{"key":"ref_8","unstructured":"(2018, January 05). Available online: https:\/\/www.bfarm.de\/SharedDocs\/Kundeninfos\/EN\/08\/2017\/04963-17_kundeninfo_en.pdf?__blob=publicationFile&v=1."},{"key":"ref_9","unstructured":"(2018, October 02). Desaf\u00edos en los M\u00e9todos de Evaluaci\u00f3n de la Sensilidad a Polimixinas (Colistina\/Polimixina b). Bolet\u00edn informativo 5 [Internet]. 2017 [citado 2 de octubre 2018] (Septiembre): 1\u201315, Available online: http:\/\/antimicrobianos.com.ar\/ATB\/wp-content\/uploads\/2017\/09\/Boletin-PCC-NAC-Nro.5-Metodos-de-Evaluacion-Sensibilidad-a-Polimixinas-Sep-20171.pdf."},{"key":"ref_10","unstructured":"Alvarez, R. Personal Communication."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1016\/j.jpeds.2014.12.023","article-title":"Reappraisal of guidelines for management of neonates with suspected early-onset sepsis","volume":"166","author":"Benitz","year":"2015","journal-title":"J. Pediatr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4067\/S0370-41062014000100007","article-title":"Diagnostic predictive scale of urinary infection in febrile neonates with no apparent focus","volume":"85","author":"Alfonso","year":"2014","journal-title":"Rev. Chil. Pediatr\u00eda"},{"key":"ref_13","first-page":"34","article-title":"Infecci\u00f3n del tracto urinario causada por Enterobacteriaceae y su relaci\u00f3n con reflujo v\u00e9sico-ureteral en reci\u00e9n nacidos","volume":"74","author":"Acosta","year":"2017","journal-title":"Bol. Med. Hosp. Infant. Mex"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1097\/INF.0000000000000110","article-title":"Urinary tract infection in outpatient febrile infants younger than 30 days of age: A 10-year evaluation","volume":"33","author":"Bonadio","year":"2014","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_15","unstructured":"Campo, A., Heredia, S., Quesada, S., Alonso, R.M., P\u00e9rez, B., and Amador, R. (2018, May 05). Infection Associated with Health Care in Newborns. Gynec Obstetric Hospital of Guanabacoa. International Health Convention, Cuba Salud 2018. [Internet] 2018 [Revisado-may-2018]. Available online: http:\/\/www.convencionsalud2018.sld.cu\/index.php\/connvencionsalud\/2018\/paper\/viewFile\/1767\/728."},{"key":"ref_16","first-page":"1028","article-title":"Clinical-epidemiological characterization of the newborn with infection associated with medical care","volume":"19","author":"Sarmiento","year":"2015","journal-title":"MEDISAN"},{"key":"ref_17","first-page":"711","article-title":"Risk factors associated with nosocomial infection in a third level Neonatal Intensive Care Unit","volume":"15","author":"Torres","year":"2015","journal-title":"Gac. Med. Mex."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s40748-017-0043-3","article-title":"Prevention and treatment of neonatal nosocomial infections","volume":"3","author":"Ramasethu","year":"2017","journal-title":"Matern. Health Neonatol. Perinatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.idc.2014.01.005","article-title":"Antimicrobial Stewardship in the NICU","volume":"28","author":"Cantey","year":"2014","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_20","first-page":"1006","article-title":"Neonatal sepsis","volume":"13","author":"Camacho","year":"2012","journal-title":"Pediatrics"},{"key":"ref_21","first-page":"60","article-title":"Severe neonatal sepsis in an Intensive Care Unit. Villa Clara","volume":"87","author":"Clemades","year":"2015","journal-title":"Rev. Cubana Pediatr\u00eda"},{"key":"ref_22","first-page":"145","article-title":"Neonatal sepsis. Characterization in very low birth weight infants. Eleven years experience","volume":"40","author":"Genes","year":"2013","journal-title":"Pediatrics"},{"key":"ref_23","first-page":"1084","article-title":"Clinical and epidemiological aspects in patients with neonatal infection","volume":"20","author":"Alfonso","year":"2016","journal-title":"MEDISAN"},{"key":"ref_24","first-page":"141","article-title":"Clinical and paraclinical characteristics of newborns with sepsis in a level IV hospital in Cali, Colombia","volume":"22","author":"Pino","year":"2018","journal-title":"Infectio"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"F257","DOI":"10.1136\/archdischild-2014-306213","article-title":"Late-onset neonatal sepsis: Recent developments","volume":"100","author":"Dong","year":"2015","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Xu, H., Dai, Q., Xu, Y., Gong, Z., Dai, G., Ding, M., Duggan, C., Hu, Z., and Hu, F.B. (2015). Time trends and risk factor associated with premature birth and infants deaths due to prematurity in Hubei Province, China from 2001 to 2012. BMC Pregnancy Childbirth, 15.","DOI":"10.1186\/s12884-015-0767-x"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s41043-018-0136-2","article-title":"Incidence and risk factors of neonatal infections in a rural Bangladeshi population: A community-based prospective study","volume":"37","author":"Mitra","year":"2018","journal-title":"J. Health Popul. Nutr."},{"key":"ref_28","first-page":"182","article-title":"Clinical, microbiological and epidemiological characterization in neonates with healthcare-related infections","volume":"88","author":"Castillo","year":"2016","journal-title":"Rev. Cubana Pediatr\u00eda"},{"key":"ref_29","first-page":"9","article-title":"Infections related to health care in Neonatal Intensive Care Units: An integrative review","volume":"16","author":"Oliveira","year":"2017","journal-title":"Enferm. Glob."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.clp.2014.10.007","article-title":"Antibiotic stewardship: Reassessment of guidelines for management of neonatal sepsis","volume":"42","author":"Cotton","year":"2015","journal-title":"Clin. Perinatol."},{"key":"ref_31","first-page":"483","article-title":"Hospital infection in newborns admitted to a neonatal intensive care service. Santiago de Cuba","volume":"14","author":"Manet","year":"2010","journal-title":"MEDISAN"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Huynh, B.T., Padget, M., Garin, B., Herindrainy, P., Kermorvant, E., Watier, L., Guillemot, D., and Delarocque-Astagneau, E. (2015). Burden of bacterial resistance among neonatal infections in low income countries: How convincing is the epidemiological evidence?. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-0843-x"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Crivaro, V., Bogdanovie, L., Bagattini, M., Iula, V.D., Catania, M., Raimondi, F., Triassi, M., and Zarrilli, R. (2015). Surveillance of healthcare-associated infections in a neonatal intensive care unit in Italy. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-0909-9"},{"key":"ref_34","first-page":"323","article-title":"Characterization of in-hospital isolates of Klebsiella pneumoniae in a tertiary hospital","volume":"54","author":"Bustamante","year":"2015","journal-title":"Rev. Cubana Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s12098-010-0272-1","article-title":"Etiology and antimicrobial resistance of neonatal sepsis at a tertiary care center in eastern India: A 3 year study","volume":"78","author":"Viswanathan","year":"2011","journal-title":"Indian J. Pediatr."},{"key":"ref_36","unstructured":"Garc\u00eda, J.L., and Varona, F. (2009). Antimicrobials: Considerations for Use in Pediatrics, Camaguey. [1st ed.]."},{"key":"ref_37","first-page":"400","article-title":"Infections with the genera Klebsiella and Acinetobacter in Cuban pediatric hospitals and antibiotic resistance","volume":"66","author":"Santisteban","year":"2014","journal-title":"Rev. Cubana Med. Trop."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"39","DOI":"10.25100\/cm.v45i1.1318","article-title":"Profile resistance of pathogens causing urinary tract infection in the pediatric population, and antibiotic treatment response, Hospital Pablo Tobon Uribe 2010\u20132011","volume":"45","author":"Serna","year":"2014","journal-title":"Colomb. Med."},{"key":"ref_39","first-page":"180","article-title":"Systematic review of drug resistance in Enterobacteriaceae from hospital isolates in Colombia","volume":"34","year":"2014","journal-title":"Biom\u00e9dica"},{"key":"ref_40","first-page":"317","article-title":"Etiology and patterns of antimicrobial resistance in early and late neonatal sepsis, in a Neonatal Intensive Care Unit","volume":"113","author":"Lona","year":"2015","journal-title":"Arch. Argent Pediatr."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Pokhrel, B., Koirala, T., Shah, G., Joshi, S., and Baral, P. (2018). Bacteriological profile and antibiotic susceptibility of neonatal sepsis in Neonatal Intensive Care Unit of a tertiary hospital in Nepal. BMC Pediatr., 18.","DOI":"10.1186\/s12887-018-1176-x"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/S0213-005X(02)72803-6","article-title":"Interpreted reading of the antibiogram of non-fermenting Gram-negative bacilli","volume":"20","author":"Vila","year":"2002","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.patbio.2007.09.018","article-title":"Stenotrophomonasmaltophilia responsible for respiratory infections in Neonatal Intensive Care Unit: Antibiotic susceptibility and molecular typing","volume":"57","author":"Abbassi","year":"2009","journal-title":"Pathol. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.5152\/ced.2015.2180","article-title":"Stenotrophomonasmaltophilia Outbreak in Neonatal Intensive Care Unit and Outbreak Management","volume":"9","author":"Cetin","year":"2015","journal-title":"J. Pediatr. Inf."}],"container-title":["Infectious Disease Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2036-7449\/13\/1\/25\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:35:23Z","timestamp":1760160923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2036-7449\/13\/1\/25"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,9]]},"references-count":44,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["idr13010025"],"URL":"https:\/\/doi.org\/10.3390\/idr13010025","relation":{},"ISSN":["2036-7449"],"issn-type":[{"value":"2036-7449","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,9]]}}}