{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:34:05Z","timestamp":1792607645225,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T00:00:00Z","timestamp":1356307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The risk to public health from the large number of dog stools present on streets of urban areas is cause for concern. Dog faeces may be a serious hazard because they may contain microorganisms that are both pathogenic to humans and resistant to several classes of antibiotics. The aim of this study was to evaluate the potential for zoonotic infections and for the presence of antibiotic resistant bacteria in canine faeces which contaminates the urban environment. A total of 418 canine faecal samples were collected from streets in seven areas of Bari, Southern Italy. We have isolated multi-drug resistant Enterococci and meticillin-resistant Staphylococcus aureus from these dog faecal samples. The presence of the resistant bacteria in an urban environment may represent a public health hazard which requires control measures by competent authorities. No Salmonella, Yersinia or Campylobacter species were isolated. Giardia cysts were detected in 1.9% of the samples. The predominant Enterococcus species were E. faecium (61.6%), E. gallinarum (23.3%) and E. casseliflavus (5.5%). Other species, including E. faecalis were also isolated. These strains were resistant to clindamycin (86.3%), tetracycline (65.7%), erythromycin (60.27%) and ampicillin (47.9%). High-level aminoglycoside resistance (HLAR) was found in 65.7% of enterococci. Resistance to three or more antibiotics and six or more antibiotics were observed in 67.12% and 38.4% of Enterococcus spp., respectively. Resistance to vancomycin and teicoplanin was not detected in any of the Enterococcus spp. isolated. Methicillin-resistant Staphylococcus aureus was isolated in 0.7% of the faecal samples. Canine faeces left on the streets may represent a risk factor for transmission of microorganisms and a reservoir of multidrug- resistant bacteria thus contributing to the spread of resistance genes into an urban area.<\/jats:p>","DOI":"10.3390\/ijerph10010072","type":"journal-article","created":{"date-parts":[[2012,12,24]],"date-time":"2012-12-24T07:41:06Z","timestamp":1356334866000},"page":"72-84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Environmental Contamination by Dog\u2019s Faeces: A Public Health Problem?"],"prefix":"10.3390","volume":"10","author":[{"given":"Vittoria","family":"Cinquepalmi","sequence":"first","affiliation":[{"name":"Department of Interdisciplinary Medicine, Occupational Medicine Section , University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Monno","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Fumarola","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianpiero","family":"Ventrella","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, University of Bari, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Calia","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Greco","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, University of Bari, Valenzano, 70010 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danila","family":"De Vito","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Soleo","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Medicine, Occupational Medicine Section , University of Bari, Piazza Giulio Cesare, 11, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2012,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1099\/00207713-37-4-327","article-title":"Yersinia rohdei sp. nov. isolated from human and dog feces and surface water","volume":"37","author":"Aleksic","year":"1987","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/j.1863-2378.2008.01145.x","article-title":"Evaluation of the risk of shedding Salmonellae and other potential pathogens by therapy dogs fed raw diets in Ontario and Alberta","volume":"55","author":"Lefebvre","year":"2008","journal-title":"Zoonoses Pub. Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/1471-2180-10-73","article-title":"Detection and quantification of 14 Campylobacter species in pet dogs reveals an increase in species richness in feces of diarrheic animals","volume":"10","author":"Chaban","year":"2010","journal-title":"BMC Microbiol."},{"key":"ref_4","first-page":"285","article-title":"Escherichia coli as a pathogen in dogs and cats","volume":"30","author":"Beutin","year":"1999","journal-title":"Vet. Res."},{"key":"ref_5","first-page":"417","article-title":"Prevalence of Giardia antigen in stool samples from dogs and cats","volume":"155","author":"Bianciardi","year":"2004","journal-title":"Rev. Med. Vet."},{"key":"ref_6","first-page":"319","article-title":"Risk of Toxocara canis eggs in stray and domestic dog hair in Egypt","volume":"10","author":"Holt","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1046\/j.1439-0450.2002.00561.x","article-title":"The importance of pets as a reservoir of resistant Enterococcus strain with special reference to vancomycin","volume":"49","author":"Rodrigues","year":"2002","journal-title":"J. Vet. Med. B Inf. Vet. Pub. Health"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1128\/AAC.40.10.2285","article-title":"Presence of vancomycin-resistant enterococci in farm pet animal","volume":"40","author":"Devriese","year":"1996","journal-title":"Antimicrob. Agent Chemother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1128\/JCM.42.3.1384-1385.2004","article-title":"Dogs should be included in surveillance programs for vancomycin-resistant enterococci","volume":"42","author":"Herrero","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1089\/107662903322541892","article-title":"Intestinal colonization by van A or van B2-Containing enterococcal isolates from healthy animals in Spain","volume":"9","author":"Torres","year":"2003","journal-title":"Microb. Drug Resist."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.vetmic.2008.04.026","article-title":"Monitoring of antimicrobial resistance in healthy dogs: First report of canine ampicillin-resistant Enterococcus faecium clonal complex 17","volume":"132","author":"Damborg","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.ijfoodmicro.2010.10.024","article-title":"The occurrence of virulence traits among high-level aminoglycosides resistant Enterococcus isolates obtained from feces of humans, animals, and birds in South Korea","volume":"144","author":"Han","year":"2011","journal-title":"Int. J. Food. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0140-6736(94)91926-7","article-title":"Human carriage of methicillin-resistant Staphylococcus aureus linked with pet dog","volume":"344","author":"Cefai","year":"1994","journal-title":"Lancet"},{"key":"ref_14","first-page":"150","article-title":"Transmission of Staphylococcus aureus between human and domestic animals","volume":"24","author":"Savelkoul","year":"1997","journal-title":"Europ. J. Clin. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e26","DOI":"10.1086\/344772","article-title":"Asyntomatic nasal carriage of mupirocin-resistant (MRSA) in a pet dog associated with MRSA infection in household contact","volume":"36","author":"Manian","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1093\/jac\/dki312","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus among staff and pets in a small animal referral hospital in the U.K","volume":"56","author":"Loeffler","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/jac\/dki471","article-title":"Molecular characterization of methicillin-resistant Sthaphylococcus aureus strain from pet animals and their relationship to human isolates","volume":"57","author":"Strommenger","year":"2006","journal-title":"J. Antimicrob. Chemoter."},{"key":"ref_18","first-page":"591","article-title":"Methicillin-resistant Staphylococcus aureus isolates from companion animals","volume":"45","author":"Rich","year":"2004","journal-title":"Vet. Rec."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.vetmic.2005.06.003","article-title":"Methicillin-resistant Staphylococcus aureus (MRSA) isolated from animals and veterinary personel in Ireland","volume":"109","author":"Abbot","year":"2005","journal-title":"Vet. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.vetmic.2007.11.011","article-title":"Extended-spectrum beta-lactamases belonging to CTX-M group produced by Escherichia coli strain isolated from companion animals treated with eurofloxacin","volume":"129","author":"Moreno","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_21","unstructured":"(1997). Eurovet Guide 1998-1999, the User\u2019s Guide to Veterinary Europe, Librairie V\u00e9t\u00e9rinaire. [2nd]."},{"key":"ref_22","unstructured":"(2006). Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard M2-A8."},{"key":"ref_23","unstructured":"Swenson, J.M., Patel, J.B., and Jorgensen, J.H. (2007). Manual of Clinical Microbiology, ASM Press. [9th]."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1128\/jcm.33.1.24-27.1995","article-title":"Detection of glycopeptides resistance and identification to the species level of clinically relevant enterococci by PCR","volume":"33","author":"Evers","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","first-page":"57","article-title":"Characterization of methicillin resistant Staphylococcus aureus (MRSA) isolated at the Policlinico Hospital of Bari (Italy)","volume":"28","author":"Corrente","year":"2005","journal-title":"New Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/JCM.01232-09","article-title":"Multiplex-PCR method for species identification of coagulase-positive staphylococci","volume":"48","author":"Sasaki","year":"2010","journal-title":"J. Clin Microbiol."},{"key":"ref_27","first-page":"4","article-title":"Accurate diagnosis of Giardia spp. and proper fecal examination procedures","volume":"7","author":"Dryden","year":"2006","journal-title":"Vet. Ther."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Berktas, M., Yaman, G., and Ozturk, O. (2008). vanC gene-related intrinsic teicoplanin resistance detected in Enterococcus casseliflavus and Enterococcus gallinarum strains by the BD Phoenix automated microbiology system. J. Clin. Microbiol.","DOI":"10.1128\/JCM.00795-08"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1177\/104063879700900424","article-title":"Salmonella shedding in racing sled dogs","volume":"9","author":"Cantor","year":"1997","journal-title":"J. Vet. Diagn. Invest."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1086\/500211","article-title":"Human health implications of Salmonella-contaminated natural pet treats and raw pet food","volume":"42","author":"Finley","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3972","DOI":"10.3390\/ijerph7113972","article-title":"Environmental monitoring and analysis of faecal contamination in an urban setting in the city of Bari (Apulia region, Italy): Health and hygiene implications","volume":"7","author":"Tarsitano","year":"2010","journal-title":"Int. J. Environ. Res. Public Health."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1136\/vr.164.2.44","article-title":"Prevalence of thermophilic Campylobacter species in household cats and dogs in Ireland","volume":"164","author":"Acke","year":"2009","journal-title":"Vet. Rec."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1007\/s00436-003-0924-4","article-title":"Prevalence of Giardia spp. in dogs and humans in Northern and Central Italy","volume":"90","author":"Capelli","year":"2003","journal-title":"Parasitol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.rvsc.2007.05.006","article-title":"Giardia and Cryptosporidium in canine faecal samples contaminating an urban area","volume":"84","author":"Rinaldi","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.prevetmed.2009.07.003","article-title":"Occurrence and cyst burden of Giardia duodenalis in dog faecal deposits from urban green areas: Implications for environmental contamination and related risks","volume":"92","author":"Papini","year":"2009","journal-title":"Prev. Vet. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1017\/S1466252308001485","article-title":"Antimicrobial resistance in companion animals","volume":"9","author":"Weese","year":"2008","journal-title":"Anim. Health Res. Rev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1111\/j.1365-2672.2009.04310.x","article-title":"Prevalence, species distribution and antimicrobial resistance of enterococci isolated from dogs and cats in the United States","volume":"107","author":"Jackson","year":"2009","journal-title":"J. Appl. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ijantimicag.2007.03.007","article-title":"Antimicrobial resistance and evaluation of susceptibility testing among pathogenic enterococci isolated from dogs and cats","volume":"30","author":"Delgado","year":"2007","journal-title":"Int. J. Antimicrob. Agent."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"231","DOI":"10.4103\/0974-777X.68534","article-title":"High-level aminoglycoside resistance and reduced susceptibility to vancomycin in nosocomial enterococci","volume":"2","author":"Adhikari","year":"2010","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cimid.2007.08.003","article-title":"Susceptibility to vancomycin and other antibiotics of 165 Enterococcus strains isolated from dogs in Italy","volume":"3","author":"Ossiprandi","year":"2008","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/jac\/dkg272","article-title":"Clinical impact of vancomycin-resistant enterococci","volume":"51","author":"Patel","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1093\/jac\/dkh332","article-title":"Pet animals as reservoirs of antimicrobial-resistant bacteria","volume":"54","author":"Guardabassi","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1128\/AAC.42.3.502","article-title":"Diversity of vanA glycopeptide resistance elements in enterococci from humans and non human sources","volume":"42","author":"Woodford","year":"1998","journal-title":"Antimicrob. Agent. Chemother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1128\/AEM.02239-06","article-title":"vanA-type enterococci from humans, animals, and food: Species distribution, population structure, Tn1546 typing and location, and virulence determinants","volume":"73","author":"Biavasco","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1093\/clinids\/12.4.644","article-title":"High-level gentamicin resistance in Enterococcus: Microbiology, genetic basis, and epidemiology","volume":"12","author":"Patterson","year":"1990","journal-title":"Rev. Infect. Dis."},{"key":"ref_46","unstructured":"Monno, R., de Carlo, C., Lapata, A., Coscia, F., de Vito, D., and Rizzo, C. (2005, January 2-5). Occurrence of Vancomycin-Resistant and High Level Amynoglycoside-Resistant Enterococcus Spp. Isolated in an Italian Hospital, Bari, Southern Italy. Proceedings of 15th European Congress of Clinical Microbiology and Infectious Diseases, Copenhagen, Denmark."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.ijfoodmicro.2010.10.024","article-title":"The occurrence of virulence traits among high-level aminoglycosides resistant Enterococcus isolates obtained from feces of humans, animals, and birds in South Korea","volume":"144","author":"Han","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1017\/S0950268809991580","article-title":"Detection of three distinct genetic lineages in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals and veterinary personnel","volume":"138","author":"Abbott","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/j.1865-1682.2011.01273.x","article-title":"Methicillin-resistant Staphylococcus aureus:An emerging pathogen of pets in Egypt with a public health burden","volume":"59","author":"Samir","year":"2012","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/jac\/dki471","article-title":"Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates","volume":"57","author":"Strommenger","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ferriera, J.P., Anderson, K.L., Correa, M.T., Lyman, R., Ruffin, F., Reller, L.B., and Fowler, V.G. (2011). Transmission of MRSA between companion animals and infected human patients presenting to outpatient medical care facilities. PLoS One.","DOI":"10.1371\/journal.pone.0026978"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1111\/j.1863-2378.2011.01448.x","article-title":"Potential for pet animals to harbour Methicillin-Resistant Staphylococcus aureus when residing with human MRSA patients","volume":"59","author":"Morris","year":"2012","journal-title":"Zoonoses Pub. Health"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Walther, B., Hermes, J., Cuny, C., Wieler, L.H., Vincze, S., Abou Elnaga, Y., Stamm, I., Kopp, P.A., Kohn, B., and Witte, W. (2012). Sharing more than friendship-Nasal colonization with coagulase-positive Staphylococci (CPS) and co-habitation aspects of dogs and their owners. PLoS One.","DOI":"10.1371\/journal.pone.0035197"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/1\/72\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:54:28Z","timestamp":1760219668000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/1\/72"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,24]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2013,1]]}},"alternative-id":["ijerph10010072"],"URL":"https:\/\/doi.org\/10.3390\/ijerph10010072","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,24]]}}}