{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:06:50Z","timestamp":1791281210150,"version":"4.1.0"},"reference-count":25,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T00:00:00Z","timestamp":1364428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption\/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 \u00b5g\u00b7mL\u22121 PC2 and 100 \u00b5g\u00b7mL\u22121 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.<\/jats:p>","DOI":"10.3390\/ijerph10041304","type":"journal-article","created":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T13:35:44Z","timestamp":1364477744000},"page":"1304-1311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes"],"prefix":"10.3390","volume":"10","author":[{"given":"Miguel","family":"Rodrigo","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Cernei","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marketa","family":"Kominkova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3,  CZ-612 42 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Zitka","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"},{"name":"Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3,  CZ-612 42 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslava","family":"Beklova","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"},{"name":"Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3,  CZ-612 42 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Zehnalek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"Kizek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"},{"name":"Lead and Cadmium Initiatives, United Nations Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic"},{"name":"Central European Institute of Technology, Brno University of Technology, Technicka 3058\/10,  CZ-616 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1104\/pp.109.4.1141","article-title":"Phytochelatins and related peptides\u2014Structure, biosynthesis, and function","volume":"109","author":"Rauser","year":"1995","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1104\/pp.123.3.825","article-title":"Phytochelatins and their roles in heavy metal detoxification","volume":"123","author":"Cobbett","year":"2000","journal-title":"Plant Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jexbot\/53.366.1","article-title":"Cellular mechanisms for heavy metal detoxification and tolerance","volume":"53","author":"Hall","year":"2002","journal-title":"J. Exp. Bot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0022-0728(02)00640-X","article-title":"Differential pulse voltammetric study of the complexation of Cd(II) by the phytochelatin (gamma-Glu-Cys)(2)Gly assisted by multivariate curve resolution","volume":"520","author":"Cruz","year":"2002","journal-title":"J. Electroanal. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/BF00425162","article-title":"Algae sequester heavy-metals via synthesis of phytochelatin complexes","volume":"150","author":"Gekeler","year":"1988","journal-title":"Arch. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"6838","DOI":"10.1073\/pnas.86.18.6838","article-title":"Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase)","volume":"86","author":"Grill","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"M28","DOI":"10.1051\/analusis:199826060028","article-title":"Application of high performance liquid chromatography\u2014Inductively coupled plasma mass spectrometry to the investigation of phytochelatin complexes and their role in heavy metal detoxification in plants","volume":"26","author":"Leopold","year":"1998","journal-title":"Analusis"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.3390\/ijerph7103804","article-title":"Effects of various doses of selenite on stinging nettle (Urtica dioica L.)","volume":"7","author":"Krystofova","year":"2010","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3789","DOI":"10.3390\/ijerph9113789","article-title":"Investigation into the effect of molds in grasses on their content of low molecular mass thiols","volume":"9","author":"Skladanka","year":"2012","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.3390\/ijerph8062401","article-title":"Selected morphological characteristics, lead uptake and phytochelatin synthesis by coffeeweed (Sesbania exaltata Raf.) grown in elevated levels of lead-contaminated soil","volume":"8","author":"Miller","year":"2011","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.chroma.2005.06.019","article-title":"Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection","volume":"1084","author":"Potesil","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1002\/jssc.200500425","article-title":"Simultaneous determination of eight biologically active thiol compounds using gradient elution-Liquid Chromatography with Coul-Array detection","volume":"29","author":"Petrlova","year":"2006","journal-title":"J. Sep. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1002\/elan.200900374","article-title":"Determination of plant thiols by liquid chromatography coupled with coulometric and amperometric detection in lettuce treated by lead(II) ions","volume":"22","author":"Diopan","year":"2010","journal-title":"Electroanalysis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.jhazmat.2011.05.088","article-title":"Phytochelatin synthase activity as a marker of metal pollution","volume":"192","author":"Zitka","year":"2011","journal-title":"J. Hazard. Mater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.jhazmat.2011.12.018","article-title":"Electrochemistry of copper(II) induced complexes in mycorrhizal maize plant tissues","volume":"203","author":"Zitka","year":"2012","journal-title":"J. Hazard. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"932","DOI":"10.3390\/s7060932","article-title":"Electroanalysis of plant thiols","volume":"7","author":"Supalkova","year":"2007","journal-title":"Sensors"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/S1452-3981(23)15080-2","article-title":"Rapid and ultrasensitive method for determination of phytochelatin(2) using high performance liquid chromatography with electrochemical detection","volume":"6","author":"Zitka","year":"2011","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.bbrc.2005.05.083","article-title":"Cadmium induces a novel metallothionein and phytochelatin 2 in an aquatic fungus","volume":"333","author":"Jaeckel","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/elan.201100578","article-title":"Voltammetric analysis of phytochelatin complexation in ternary metal mixtures supported by multivariate analysis and ESI-MS","volume":"24","author":"Cavanillas","year":"2012","journal-title":"Electroanalysis"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1039\/b707830g","article-title":"SEC-ICP-MS and ESI-MS\/MS for analyzing in vitro and in vivo Cd-phytochelatin complexes in a Cd-hyperaccumulator Brassica chinensis","volume":"22","author":"Chen","year":"2007","journal-title":"J. Anal. At. Spectrom."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.indcrop.2011.11.008","article-title":"Cadmium-induced production of phytochelatins and speciation of intracellular cadmium in organs of Linum usitatissimum seedlings","volume":"36","author":"Najmanova","year":"2012","journal-title":"Ind. Crop. Prod."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1104\/pp.103.033506","article-title":"The nature of arsenic-phytochelatin complexes in Holcus lanatus and Pteris cretica","volume":"134","author":"Raab","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1080\/10739149.2011.651669","article-title":"Review: Liquid chromatography-post-column derivatization for amino acid analysis: Strategies, instrumentation, and applications","volume":"40","author":"Rigas","year":"2012","journal-title":"Instrum. Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1745-4514.2002.tb00754.x","article-title":"Characterization of phytochelatin-like complexes from flax (Linum usitatissimum) seed","volume":"26","author":"Sultanbawa","year":"2002","journal-title":"J. Food Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Nejdl, L., Sochor, J., Zitka, O., Cernei, N., Ruttkay-Nedecky, B., Kopel, P., Babula, P., Adam, V., Hubalek, J., and Kizek, R. (2013). Spectrometric and chromatographic study of reactive oxidants hypochlorous and hypobromous acids and their interactions with taurine. Chromatographia, in press.","DOI":"10.1007\/s10337-012-2354-x"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/4\/1304\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:45:51Z","timestamp":1760219151000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/4\/1304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,28]]},"references-count":25,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,4]]}},"alternative-id":["ijerph10041304"],"URL":"https:\/\/doi.org\/10.3390\/ijerph10041304","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,28]]}}}