{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:44:47Z","timestamp":1787028287628,"version":"build-2736575974"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>A total of 110 strains of Klebsiella pneumoniae were used to optimize  pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pneumoniae. By software analysis and pilot study, AvrII was chosen as another PFGE enzyme. Both XbaI- and AvrII-PFGE gave D-values higher than 0.99 for 69 K. pneumoniae isolated from different sources. Our results also showed good typeability, reproducibility of both XbaI- and AvrII-PFGE for K. pneumoniae subtyping. Furthermore, the established PFGE method also had good discriminatory power to distinguish outbreak K. pneumoniae strains and a high degree of consistency with multilocus sequence typing method. A rapid PFGE protocol was established here, which could be used for genotyping and other researches of  K. pneumoniae.<\/jats:p>","DOI":"10.3390\/ijerph10072720","type":"journal-article","created":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T11:23:17Z","timestamp":1372677797000},"page":"2720-2731","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae"],"prefix":"10.3390","volume":"10","author":[{"given":"Hui","family":"Han","sequence":"first","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijian","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haishan","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Lu","sequence":"additional","affiliation":[{"name":"Gaoling Center for Diseases Control and Prevention, Xi'an 710200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kongxin","family":"Hu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoliang","family":"Xu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine, Beijing 100123, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1136\/gut.50.3.420","article-title":"A global emerging disease of Klebsiella pneumoniae liver abscess: Is serotype K1 an important factor for complicated endophthalmitis?","volume":"50","author":"Fung","year":"2002","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3201\/eid0802.010025","article-title":"Community-acquired Klebsiella pneumoniae bacteremia: Global differences in clinical patterns","volume":"8","author":"Ko","year":"2002","journal-title":"Emerg. 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Oswaldo Cruz"}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/7\/2720\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:47:38Z","timestamp":1760219258000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/7\/2720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,1]]},"references-count":25,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2013,7]]}},"alternative-id":["ijerph10072720"],"URL":"https:\/\/doi.org\/10.3390\/ijerph10072720","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,1]]}}}