{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:43:27Z","timestamp":1791287007177,"version":"4.1.0"},"reference-count":188,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T00:00:00Z","timestamp":1384214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>In Europe, many flaviviruses are endemic (West Nile, Usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). Due to the temporal and geographical co-circulation of flaviviruses in Europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. Serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the Flavivirus genus. Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. Serological assay results should thus be interpreted with care and confirmed by comparative neutralization tests using a panel of viruses known to circulate in Europe. However, antibody cross-reactivity could be advantageous in efforts to control emerging flaviviruses because it ensures partial cross-protection. In contrast, it might also facilitate subsequent diseases, through a phenomenon called antibody-dependent enhancement mainly described for dengue virus infections. Here, we review the serological methods commonly used in WNV diagnosis and surveillance in Europe. By examining past and current epidemiological situations in different European countries, we present the challenges involved in interpreting flavivirus serological tests and setting up appropriate surveillance programs; we also address the consequences of flavivirus circulation and vaccination for host immunity.<\/jats:p>","DOI":"10.3390\/ijerph10116049","type":"journal-article","created":{"date-parts":[[2013,11,12]],"date-time":"2013-11-12T10:43:07Z","timestamp":1384252987000},"page":"6049-6083","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":167,"title":["Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease"],"prefix":"10.3390","volume":"10","author":[{"given":"C\u00e9cile","family":"Beck","sequence":"first","affiliation":[{"name":"UMR1161 Virologie INRA, ANSES, ENVA, EU-RL on equine West Nile disease, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2125-9743","authenticated-orcid":false,"given":"Miguel","family":"Jimenez-Clavero","sequence":"additional","affiliation":[{"name":"CISA-INIA, Valdeolmos (Madrid) 28130, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Leblond","sequence":"additional","affiliation":[{"name":"D\u00e9partement Hippique, VetAgroSup, Marcy l'Etoile 69280, France"},{"name":"UR346, INRA, Saint Gen\u00e8s Champanelle 63122, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Durand","sequence":"additional","affiliation":[{"name":"Epidemiology Unit, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Nowotny","sequence":"additional","affiliation":[{"name":"Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology,  University of Veterinary Medicine Vienna, Vienna 1210, Austria"},{"name":"Department of Microbiology and Immunology, College of Medicine and Health Sciences,  Sultan Qaboos University, Muscat 123, Sultanate of Oman"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Leparc-Goffart","sequence":"additional","affiliation":[{"name":"IRBA, Marseille 13384, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phan","family":"Zientara","sequence":"additional","affiliation":[{"name":"UMR1161 Virologie INRA, ANSES, ENVA, EU-RL on equine West Nile disease, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Jourdain","sequence":"additional","affiliation":[{"name":"UR346, INRA, Saint Gen\u00e8s Champanelle 63122, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Lecollinet","sequence":"additional","affiliation":[{"name":"UMR1161 Virologie INRA, ANSES, ENVA, EU-RL on equine West Nile disease, Animal Health Laboratory, ANSES, Maisons-Alfort 94704, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2013,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1128\/CMR.00045-12","article-title":"West Nile Virus: Biology, transmission, and human infection","volume":"25","author":"Colpitts","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.cimid.2004.03.009","article-title":"West Nile: Worldwide current situation in animals and humans","volume":"27","author":"Dauphin","year":"2004","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1111\/j.1865-1682.2010.01196.x","article-title":"Clinical diagnosis of West Nile Fever in Equids by classification and regression tree (CART) analysis and comparative study of clinical appearance in three European countries","volume":"58","author":"Porter","year":"2011","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5563","DOI":"10.1016\/j.vaccine.2006.12.005","article-title":"West Nile virus: Recent trends in diagnosis and vaccine development","volume":"25","author":"Dauphin","year":"2007","journal-title":"Vaccine"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1099\/0022-1317-23-1-91","article-title":"The flaviviruses (group B arboviruses): A cross-neutralization study","volume":"23","author":"Porterfield","year":"1974","journal-title":"J. Gen. Virol."},{"key":"ref_6","unstructured":"Centers for Disease Control and Prevention. Available online:http:\/\/www.cdc.gov\/westnile\/index.html."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1126\/science.1201010","article-title":"Globalization, land use, and the invasion of West Nile virus","volume":"334","author":"Kilpatrick","year":"2011","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1051\/vetres\/2010039","article-title":"West Nile virus and its emergence in the United States of America","volume":"41","author":"Murray","year":"2010","journal-title":"Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/nature05829","article-title":"West Nile virus emergence and large-scale declines of North American bird populations","volume":"447","author":"LaDeau","year":"2007","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1017\/S1466252307001430","article-title":"Transmission dynamics and changing epidemiology of West Nile virus","volume":"9","author":"Blitvich","year":"2008","journal-title":"Anim. Health Res. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Brault, A.C. (2009). Changing patterns of West Nile virus transmission: Altered vector competence and host susceptibility. Vet. Res., 40.","DOI":"10.1051\/vetres\/2009026"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.vetmic.2012.12.043","article-title":"Experimental infection of Carrion crows (Corvus corone) with two European West Nile virus (WNV) strains","volume":"165","author":"Dridi","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.3201\/eid1012.040486","article-title":"Differential virulence of West Nile strains for American crows","volume":"10","author":"Brault","year":"2004","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"19508:1","DOI":"10.2807\/ese.15.10.19508-en","article-title":"West Nile virus in Europe: Understanding the present to gauge the future","volume":"15","author":"Reiter","year":"2010","journal-title":"Euro. Surveill."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1126\/science.1094247","article-title":"Emerging vectors in the Culex pipiens complex","volume":"303","author":"Fonseca","year":"2004","journal-title":"Science"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1017\/S0950268803008707","article-title":"Implications for Northern Europe of the emergence of West Nile virus in the USA","volume":"131","author":"Gould","year":"2003","journal-title":"Epidemiol. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Swaddle, J.P., and Calos, S.E. (2008). Increased avian diversity is associated with lower incidence of human West Nile infection: Observation of the dilution effect. PloS One, 3.","DOI":"10.1371\/journal.pone.0002488"},{"key":"ref_18","first-page":"109","article-title":"Avian diversity and West Nile virus: Testing associations between biodiversity and infectious disease risk","volume":"273","author":"Ezenwa","year":"2006","journal-title":"Proc. Biol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1136\/vr.157.22.711","article-title":"Serosurvey for West Nile virus in horses in southern France","volume":"157","author":"Durand","year":"2005","journal-title":"Vet. Rec."},{"key":"ref_20","unstructured":"West Nile Fever. Available online:http:\/\/ecdc.europa.eu\/en\/healthtopics\/west_nile_fever\/Pages\/index.aspx."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"829","DOI":"10.20506\/rst.31.3.2167","article-title":"West Nile virus epidemiology and factors triggering change in its distribution in Europe","volume":"31","author":"Pradier","year":"2012","journal-title":"Rev. Sci. Tech."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1089\/vbz.2009.0232","article-title":"Incidence of West Nile virus in birds arriving in wildlife rehabilitation centers in southern Spain","volume":"11","author":"Lopez","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"615","DOI":"10.3201\/eid0704.017402","article-title":"Crow deaths as a sentinel surveillance system for West Nile virus in the northeastern United States, 1999","volume":"7","author":"Eidson","year":"2001","journal-title":"Emerg. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.vetmic.2010.12.012","article-title":"Detection and molecular analysis of West Nile virus infections in birds of prey in the eastern part of Austria in 2008 and 2009","volume":"149","author":"Wodak","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1089\/vbz.2010.0234","article-title":"West Nile virus in Europe: A comparison of surveillance system designs in a changing epidemiological context","volume":"11","author":"Chevalier","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19547:1","DOI":"10.2807\/ese.15.16.19547-en","article-title":"West Nile virus circulation in Emilia-Romagna, Italy: The integrated surveillance system 2009","volume":"15","author":"Angelini","year":"2010","journal-title":"Euro. Surveill."},{"key":"ref_27","unstructured":"Calzolari, M., Monaco, F., Montarsi, F., Bonilauri, P., Ravagnan, S., Bellini, R., Cattoli, G., Cordioli, P., Cazzin, S., and Pinoni, C. (2013). New incursions of West Nile virus lineage 2 in Italy in 2013: The value of the entomological surveillance as early warning system. Vet. Ital., 2013."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1089\/vbz.2012.1176","article-title":"Detection and early warning of West Nile Virus circulation in central Macedonia, Greece, using sentinel chickens and mosquitoes","volume":"13","author":"Chaskopoulou","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1373\/clinchem.2005.064451","article-title":"First international proficiency study on West Nile virus molecular detection","volume":"52","author":"Niedrig","year":"2006","journal-title":"Clin. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3201\/eid1603.091033","article-title":"Putative new lineage of west nile virus, Spain","volume":"16","author":"Vazquez","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"618","DOI":"10.3201\/eid1204.051379","article-title":"Lineage 1 and 2 strains of encephalitic West Nile Virus, central Europe","volume":"12","author":"Bakonyi","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3201\/eid1102.041028","article-title":"Novel flavivirus or new lineage of West Nile virus, central Europe","volume":"11","author":"Bakonyi","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3201\/eid0604.000408","article-title":"Isolation of two strains of West Nile virus during an outbreak in southern Russia, 1999","volume":"6","author":"Lvov","year":"2000","journal-title":"Emerg. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"19762:1","DOI":"10.2807\/ese.16.02.19762-en","article-title":"Outbreak of West Nile virus infection in humans, Romania, July to October 2010","volume":"16","author":"Sirbu","year":"2011","journal-title":"Euro. Surveill."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2964","DOI":"10.1128\/JVI.01963-10","article-title":"Phylogeography of West Nile virus: From the cradle of evolution in Africa to Eurasia, Australia, and the Americas","volume":"85","author":"May","year":"2011","journal-title":"J. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"S98","DOI":"10.1038\/nm1144","article-title":"Emerging flaviviruses: The spread and resurgence of Japanese encephalitis, West Nile and dengue viruses","volume":"10","author":"Mackenzie","year":"2004","journal-title":"Nat. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1016\/j.vaccine.2011.09.114","article-title":"Flaviviruses and flavivirus vaccines","volume":"30","author":"Heinz","year":"2012","journal-title":"Vaccine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1099\/vir.0.031641-0","article-title":"Flavivirus-induced antibody cross-reactivity","volume":"92","author":"Mansfield","year":"2011","journal-title":"J. Gen. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/S1286-4579(02)01647-7","article-title":"Reactivity of serum samples from patients with a flavivirus infection measured by immunofluorescence assay and ELISA","volume":"4","author":"Koraka","year":"2002","journal-title":"Microbes Infect."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"635","DOI":"10.3201\/eid1904.121450","article-title":"Tick-borne encephalitis virus in horses, Austria, 2011","volume":"19","author":"Rushton","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.3390\/ijerph10083112","article-title":"Use of competition ELISA for monitoring of West Nile virus infections in horses in Germany","volume":"10","author":"Ziegler","year":"2013","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"ref_42","unstructured":"ICTV 2012 Master Species List (MSL). Available online:http:\/\/www.ictvonline.org\/."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1099\/0022-1317-82-8-1867","article-title":"Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography","volume":"82","author":"Gaunt","year":"2001","journal-title":"J. Gen. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JVI.72.1.73-83.1998","article-title":"Phylogeny of the genus Flavivirus","volume":"72","author":"Kuno","year":"1998","journal-title":"J. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1099\/0022-1317-70-1-37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera","volume":"70","author":"Calisher","year":"1989","journal-title":"J. Gen. Virol."},{"key":"ref_46","unstructured":"Gould, E.A., Moss, S.R., and Turner, S.L. (2004). Emergence and Control of Zoonotic Viral Encephalitis, Les Pensi\u00e8res, France 6\u20138 April 2003, Springer-Verlag Wien."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1099\/vir.0.036525-0","article-title":"Molecular evolution of the insect-specific flaviviruses","volume":"93","author":"Cook","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/S0140-6736(00)02036-5","article-title":"Neurological manifestations of dengue infection","volume":"355","author":"Solomon","year":"2000","journal-title":"Lancet"},{"key":"ref_49","unstructured":"Saluzzo, J.F., and Dodet, B. (1997). Factors in the Emergence of Arbovirus Diseases, Elsevier."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/j.1863-2378.2010.01342.x","article-title":"Confirmed exposure to tick-borne encephalitis virus and probable human cases of tick-borne encephalitis in Central\/Northern Anatolia, Turkey","volume":"58","author":"Ergunay","year":"2011","journal-title":"Zoonoses Public Health"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ttbdis.2010.10.007","article-title":"Tick-borne encephalitis 2010: Epidemiology, risk areas, and virus strains in Europe and Asia\u2014An overview","volume":"2","author":"Suss","year":"2011","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.vetmic.2009.08.024","article-title":"Zoonotic tick-borne flaviviruses","volume":"140","author":"Dobler","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0368-1742(94)80052-9","article-title":"Louping-ill in sheep","volume":"7","year":"1894","journal-title":"J. Comp. Pathol. Ther."},{"key":"ref_55","unstructured":"Monath, T.P. (1988). The Arboviruses: Epidemiology and Ecology, Vol. III, CRC Press, Inc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF01309911","article-title":"The isolation of Meaban virus, a new Flavivirus from the seabird tick Ornithodoros (Alectorobius) maritimus in France","volume":"83","author":"Chastel","year":"1985","journal-title":"Arch. Virol."},{"key":"ref_57","unstructured":"Hubalek, Z., and Halouzka, J. (1996). Arthropod-Borne Viruses of Vertebrates in Europe, Institute of Landscape Ecology of the Academy of Sciences of the Czech Republic."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetmic.2009.08.025","article-title":"Zoonotic mosquito-borne flaviviruses: Worldwide presence of agents with proven pathogenicity and potential candidates of future emerging diseases","volume":"140","author":"Weissenbock","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"423","DOI":"10.4269\/ajtmh.1969.18.423","article-title":"Survey for antibodies to arboviruses in serum of animals from southern Portugal","volume":"18","author":"Filipe","year":"1969","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_60","first-page":"239","article-title":"Epidemiology of the West Nile virus: Study of a focus in Camargue. IV. Meningo-encephalomyelitis of the horse","volume":"118","author":"Joubert","year":"1970","journal-title":"Ann. Inst. Pasteur (Paris)"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3201\/eid0804.010217","article-title":"Introduction of West Nile virus in the Middle East by migrating white storks","volume":"8","author":"Malkinson","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"20","DOI":"10.2807\/ese.15.34.19644-en","article-title":"Ongoing outbreak of West Nile virus infections in humans in Greece, July\u2013August 2010","volume":"15","author":"Papa","year":"2010","journal-title":"Euro. Surveill."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetmic.2013.03.005","article-title":"Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008\/2009","volume":"165","author":"Bakonyi","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_64","first-page":"115","article-title":"Epidemiologic situation of West Nile fever in Russia in 2010","volume":"3","author":"Onishchenko","year":"2011","journal-title":"Zh. Mikrobiol. Epidemiol. Immunobiol."},{"key":"ref_65","first-page":"471","article-title":"Further spread of West Nile virus in Italy","volume":"46","author":"Calistri","year":"2010","journal-title":"Vet. Ital."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"274","DOI":"10.3201\/eid1902.121191","article-title":"Usutu virus, Italy, 1996","volume":"19","author":"Weissenbock","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"652","DOI":"10.3201\/eid0807.020094","article-title":"Emergence of Usutu virus, an African mosquito-borne flavivirus of the Japanese encephalitis virus group, central Europe","volume":"8","author":"Weissenbock","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.vetmic.2007.08.023","article-title":"Serological evidence of continuing high Usutu virus (Flaviviridae) activity and establishment of herd immunity in wild birds in Austria","volume":"127","author":"Meister","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"e32604","DOI":"10.1371\/journal.pone.0032604","article-title":"Epizootic emergence of Usutu virus in wild and captive birds in Germany","volume":"7","author":"Becker","year":"2012","journal-title":"PloS One"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"861","DOI":"10.3201\/eid1405.071577","article-title":"Usutu virus sequences in Culex pipiens (Diptera : Culicidae), Spain","volume":"14","author":"Busquets","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.3201\/eid1907.130199","article-title":"Usuto virus in migratory song thrushes, Spain","volume":"19","author":"Gamino","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Hubalek, Z., Rudolf, I., Capek, M., Bakonyi, T., Betasova, L., and Nowotny, N. (2012). Usutu virus in Blackbirds (Turdus merula), Czech Republic, 2011\u20132012. Emerg. Dis., 2012.","DOI":"10.1111\/tbed.12025"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1128\/JCM.01390-07","article-title":"Emergence of Usutu virus in Hungary","volume":"45","author":"Bakonyi","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.vetmic.2009.07.036","article-title":"Usutu virus in wild birds in northern Italy","volume":"141","author":"Manarolla","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetmic.2010.09.018","article-title":"Emergence and establishment of Usutu virus infection in wild and captive avian species in and around Zurich, Switzerland\u2014Genomic and pathologic comparison to other central European outbreaks","volume":"148","author":"Steinmetz","year":"2011","journal-title":"Vet. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1089\/vbz.2012.1166","article-title":"Recent circulation of West Nile virus and potentially other closely related flaviviruses in Southern France","volume":"13","author":"Vittecoq","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1089\/vbz.2012.1236","article-title":"Demonstration of Usutu virus antibodies in Horses, Croatia","volume":"13","author":"Barbic","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_78","first-page":"1498","article-title":"Bagaza virus in partridges and pheasants, Spain, 2010","volume":"17","author":"Aguero","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1099\/vir.0.012336-0","article-title":"Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients","volume":"90","author":"Bondre","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/1756-3305-5-99","article-title":"Evidence of mosquito-transmitted flavivirus circulation in Piedmont, north-western Italy","volume":"5","author":"Cerutti","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1089\/vbz.2008.0188","article-title":"Surveillance of arboviruses in Spanish wetlands: Detection of new flavi- and phleboviruses","volume":"10","author":"Vazquez","year":"2010","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"20221:1","DOI":"10.2807\/ese.17.28.20221-en","article-title":"Japanese encephalitis virus RNA detected in Culex pipiens mosquitoes in Italy","volume":"17","author":"Ravanini","year":"2012","journal-title":"Euro. Surveill."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"9532","DOI":"10.1128\/JVI.00529-09","article-title":"Characterization of a novel flavivirus from mosquitoes in northern europe that is related to mosquito-borne flaviviruses of the tropics","volume":"83","author":"Huhtamo","year":"2009","journal-title":"J. Virol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"e14324","DOI":"10.1371\/journal.pone.0014324","article-title":"Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009","volume":"5","author":"Calzolari","year":"2010","journal-title":"PloS One"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.3201\/eid1906.130122","article-title":"Flaviviruses in game birds, Southern Spain, 2011\u20132012","volume":"19","author":"Llorente","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1089\/vbz.2010.0176","article-title":"Assessing the risks of West Nile virus-infected mosquitoes from transatlantic aircraft: Implications for disease emergence in the United Kingdom","volume":"12","author":"Brown","year":"2012","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1089\/vbz.2006.0543","article-title":"Bird species potentially involved in introduction, amplification, and spread of West Nile virus in a Mediterranean wetland, the Camargue (Southern France)","volume":"7","author":"Jourdain","year":"2007","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.antiviral.2009.10.008","article-title":"Present and future arboviral threats","volume":"85","author":"Weaver","year":"2010","journal-title":"Antivir. Res."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1111\/j.1523-1739.2006.00423.x","article-title":"Predicting pathogen introduction: West Nile virus spread to Galaipagos","volume":"20","author":"Kilpatrick","year":"2006","journal-title":"Conserv. Biol."},{"key":"ref_90","first-page":"394","article-title":"A quantitative risk assessment of West Nile virus introduction into Barbados","volume":"56","author":"Douglas","year":"2007","journal-title":"West Indian Med. J."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1098\/rsif.2012.0138","article-title":"Suitability of European climate for the Asian tiger mosquito Aedes albopictus: Recent trends and future scenarios","volume":"9","author":"Caminade","year":"2012","journal-title":"J. R. Soc. Interface\/R. Soc."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1111\/1469-0691.12189","article-title":"Public health significance of invasive mosquitoes in Europe","volume":"19","author":"Schaffner","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_93","first-page":"19805:1","article-title":"Autochthonous dengue fever in Croatia, August-September 2010","volume":"16","author":"Aleraj","year":"2011","journal-title":"Euro. Surveill."},{"key":"ref_94","first-page":"19676:1","article-title":"First two autochthonous dengue virus infections in metropolitan France, September 2010","volume":"15","author":"Souares","year":"2010","journal-title":"Euro. Surveill."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1159\/000328320","article-title":"West Nile virus: The complex biology of an emerging pathogen","volume":"54","author":"Ulbert","year":"2011","journal-title":"Intervirology"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1297-9716-43-16","article-title":"Recent progress in West Nile virus diagnosis and vaccination","volume":"43","author":"Ulbert","year":"2012","journal-title":"Vet. Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"498","DOI":"10.2746\/042516407X248476","article-title":"A case-control study of factors associated with development of clinical disease due to West Nile virus, Saskatchewan 2003","volume":"39","author":"Epp","year":"2007","journal-title":"Equine Vet. J."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"4608","DOI":"10.1016\/j.vaccine.2011.04.058","article-title":"Protection provided by a recombinant ALVAC\u00ae-WNV vaccine expressing the prM\/E genes of a lineage 1 strain of WNV against a virulent challenge with a lineage 2 strain","volume":"29","author":"Minke","year":"2011","journal-title":"Vaccine"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"3856","DOI":"10.1016\/j.vaccine.2013.06.059","article-title":"Inactivated West Nile Virus (WNV) vaccine, Duvaxyn WNV, protects against a highly neuroinvasive lineage 2 WNV strain in mice","volume":"31","author":"Venter","year":"2013","journal-title":"Vaccine"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"4955","DOI":"10.1016\/j.vaccine.2005.03.052","article-title":"An inactivated West Nile virus vaccine for domestic geese-efficacy study and a summary of 4 years of field application","volume":"23","author":"Samina","year":"2005","journal-title":"Vaccine"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1016\/j.vaccine.2006.11.056","article-title":"Prospective immunization of the endangered California condors (Gymnogyps californianus) protects this species from lethal West Nile virus infection","volume":"25","author":"Chang","year":"2007","journal-title":"Vaccine"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1089\/vbz.2010.0171","article-title":"Surveillance for West Nile virus and vaccination of free-ranging island scrub-jays (Aphelocoma insularis) on Santa Cruz Island, California","volume":"11","author":"Boyce","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1637\/0005-2086(2007)51[573:DVOTAC]2.0.CO;2","article-title":"DNA vaccination of the American crow (Corvus brachyrhynchos) provides partial protection against lethal challenge with West Nile virus","volume":"51","author":"Bunning","year":"2007","journal-title":"Avian Dis."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.3201\/eid0909.030025","article-title":"DNA vaccine for West Nile virus infection in fish crows (Corvus ossifragus)","volume":"9","author":"Turell","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1089\/vbz.2009.0029","article-title":"DNA vaccination of American robins (Turdus migratorius) against West Nile virus","volume":"10","author":"Kilpatrick","year":"2010","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.coi.2009.05.018","article-title":"Yellow fever vaccine\u2014How does it work and why do rare cases of serious adverse events take place?","volume":"21","author":"Barrett","year":"2009","journal-title":"Curr. Opin. Immunol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1086\/590116","article-title":"Randomized, double-blind, placebo-controlled phase 3 trial of the safety and tolerability of IC51, an inactivated Japanese encephalitis vaccine","volume":"198","author":"Tauber","year":"2008","journal-title":"J. Infect. Dis."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1016\/S0140-6736(07)61780-2","article-title":"Safety and immunogenicity of a Vero-cell-derived, inactivated Japanese encephalitis vaccine: A non-inferiority, phase III, randomised controlled trial","volume":"370","author":"Tauber","year":"2007","journal-title":"Lancet"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"7559","DOI":"10.1016\/j.vaccine.2007.08.024","article-title":"Field effectiveness of vaccination against tick-borne encephalitis","volume":"25","author":"Heinz","year":"2007","journal-title":"Vaccine"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"561","DOI":"10.4269\/ajtmh.1958.7.561","article-title":"Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses","volume":"7","author":"Clarke","year":"1958","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_111","unstructured":"Luca, V. (2011). The Structural Basis of Flaviviridae Interaction with Antibodies and Receptors, Washington University."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.virol.2005.02.020","article-title":"Characterization of neutralizing antibodies to West Nile virus","volume":"336","author":"Sanchez","year":"2005","journal-title":"Virology"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/S0966-842X(03)00005-2","article-title":"The importance of being outer: Consequences of the distinction between the outer and inner surfaces of flavivirus glycoprotein E","volume":"11","author":"Seligman","year":"2003","journal-title":"Trends Microbiol."},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Pierson, T.C., and Diamond, M.S. (2008). Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection. Expert Rev. Mol. Med., 10.","DOI":"10.1017\/S1462399408000665"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1038\/sj.emboj.7600064","article-title":"Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation","volume":"23","author":"Bressanelli","year":"2004","journal-title":"EMBO J."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1600-065X.2008.00676.x","article-title":"The structural immunology of antibody protection against West Nile virus","volume":"225","author":"Diamond","year":"2008","journal-title":"Immunol. Rev."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"13975","DOI":"10.1128\/JVI.78.24.13975-13986.2004","article-title":"Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes","volume":"78","author":"Crill","year":"2004","journal-title":"J. Virol."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1038\/nature03956","article-title":"Structural basis of West Nile virus neutralization by a therapeutic antibody","volume":"437","author":"Nybakken","year":"2005","journal-title":"Nature"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"13097","DOI":"10.1128\/JVI.76.24.13097-13100.2002","article-title":"Identification of neutralizing epitopes within structural domain III of the West Nile virus envelope protein","volume":"76","author":"Beasley","year":"2002","journal-title":"J. Virol."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"11828","DOI":"10.1128\/JVI.00643-07","article-title":"Induction of epitope-specific neutralizing antibodies against West Nile virus","volume":"81","author":"Oliphant","year":"2007","journal-title":"J. Virol."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"9557","DOI":"10.1128\/JVI.00080-06","article-title":"Cryptic properties of a cluster of dominant flavivirus cross-reactive antigenic sites","volume":"80","author":"Stiasny","year":"2006","journal-title":"J. Virol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"11567","DOI":"10.1128\/JVI.05859-11","article-title":"Poorly neutralizing cross-reactive antibodies against the fusion loop of West Nile virus envelope protein protect in vivo via Fcgamma receptor and complement-dependent effector mechanisms","volume":"85","author":"Vogt","year":"2011","journal-title":"J. Virol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1586\/erv.11.180","article-title":"Feasibility of cross-protective vaccination against flaviviruses of the Japanese encephalitis serocomplex","volume":"11","author":"Lobigs","year":"2012","journal-title":"Expert Rev. Vaccines"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0168-1702(91)90025-Q","article-title":"Glycosylation and secretion of yellow fever virus nonstructural protein NS1","volume":"18","author":"Post","year":"1991","journal-title":"Virus Res."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"6104","DOI":"10.1128\/JVI.73.7.6104-6110.1999","article-title":"Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion","volume":"73","author":"Flamand","year":"1999","journal-title":"J. Virol."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"9551","DOI":"10.1128\/JVI.00879-07","article-title":"Antibody recognition of cell surface-associated NS1 triggers Fc-\u03b3 receptor-mediated phagocytosis and clearance of West Nile Virus-infected cells","volume":"81","author":"Chung","year":"2007","journal-title":"J. Virol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1586\/14787210.2013.814824","article-title":"Application of West Nile virus diagnostic techniques","volume":"11","author":"Hirota","year":"2013","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1128\/jcm.4.6.503-510.1976","article-title":"Serum dilution neutralization test for California group virus identification and serology","volume":"4","author":"Lindsey","year":"1976","journal-title":"J. Clin. Microbiol."},{"key":"ref_129","unstructured":"OIE (2008). OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Office International des Epizooties. [6th ed.]."},{"key":"ref_130","first-page":"128","article-title":"Comparison of assays for the detection of West Nile virus antibodies in chicken serum","volume":"67","author":"Weingartl","year":"2003","journal-title":"Can. J. Vet. Res.-Rev. Can. Rech. Veterinaire"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1099\/vir.0.19341-0","article-title":"Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK","volume":"84","author":"Buckley","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1089\/vim.2007.0111","article-title":"Serologic survey of potential vertebrate hosts for West Nile virus in Poland","volume":"21","author":"Hubalek","year":"2008","journal-title":"Viral. Immunol."},{"key":"ref_133","doi-asserted-by":"crossref","unstructured":"Hobson-Peters, J. (2012). Approaches for the development of rapid serological assays for surveillance and diagnosis of infections caused by zoonotic flaviviruses of the Japanese encephalitis virus serocomplex. J Biomed. Biotechnol., 2012.","DOI":"10.1155\/2012\/379738"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jviromet.2006.09.009","article-title":"Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation","volume":"139","author":"Niedrig","year":"2007","journal-title":"J. Virol. Methods"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/JCM.38.5.1823-1826.2000","article-title":"Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections","volume":"38","author":"Martin","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1128\/JCM.38.6.2232-2239.2000","article-title":"Evaluation of immunoglobulin M (IgM) and IgG enzyme immunoassays in serologic diagnosis of West Nile virus infection","volume":"38","author":"Tardei","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_137","doi-asserted-by":"crossref","unstructured":"Niedrig, M., Donoso-Mantke, O., Altmann, D., and Zeller, H. (2007). First international diagnostic accuracy study for the serological detection of West Nile virus infection. BMC Infect. Dis., 7.","DOI":"10.1186\/1471-2334-7-72"},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jviromet.2010.02.013","article-title":"Evaluation of an enzyme-linked immunosorbent assay for detection of West Nile virus infection based on a recombinant envelope protein produced in Trichoplusia ni larvae","volume":"166","author":"Blazquez","year":"2010","journal-title":"J. Virol. Methods"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jviromet.2011.03.015","article-title":"Development and evaluation of a new epitope-blocking ELISA for universal detection of antibodies to West Nile virus","volume":"174","author":"Sotelo","year":"2011","journal-title":"J. Virol. Methods"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"4101","DOI":"10.1128\/JCM.42.9.4101-4110.2004","article-title":"Use of recombinant E protein domain III-based enzyme-linked immunosorbent assays for differentiation of tick-borne encephalitis serocomplex flaviviruses from mosquito-borne flaviviruses","volume":"42","author":"Holbrook","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1128\/JCM.42.6.2759-2765.2004","article-title":"Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection","volume":"42","author":"Beasley","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1128\/CVI.00051-07","article-title":"Epitope-blocking enzyme-linked immunosorbent assay to differentiate west nile virus from Japanese encephalitis virus infections in equine sera","volume":"14","author":"Kitai","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1128\/JCM.41.3.1041-1047.2003","article-title":"Epitope-blocking enzyme-linked immunosorbent assays for the detection of serum antibodies to west nile virus in multiple avian species","volume":"41","author":"Blitvich","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"192","DOI":"10.3109\/13813455.2012.705301","article-title":"Bio-Rad\u2019s Bio-Plex\u00ae suspension array system, xMAP technology overview","volume":"118","author":"Houser","year":"2012","journal-title":"Arch. Physiol. Biochem."},{"key":"ref_145","doi-asserted-by":"crossref","unstructured":"Lelli, D., Moreno, A., Brocchi, E., Sozzi, E., Capucci, L., Canelli, E., Barbieri, I., Zeller, H., and Cordioli, P. (2012). West Nile virus: Characterization and diagnostic applications of monoclonal antibodies. Virol. J., 9.","DOI":"10.1186\/1743-422X-9-81"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1056\/NEJM200106143442401","article-title":"The outbreak of West Nile virus infection in the New York city area in 1999","volume":"344","author":"Nash","year":"2001","journal-title":"N. Engl. J. Med."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"370","DOI":"10.3201\/eid0604.000407","article-title":"The role of pathology in an investigation of an outbreak of West Nile encephalitis in New York, 1999","volume":"6","author":"Shieh","year":"2000","journal-title":"Emerg. Infect. Dis."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1126\/science.286.5448.2333","article-title":"Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States","volume":"286","author":"Lanciotti","year":"1999","journal-title":"Science"},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1128\/CVI.00201-10","article-title":"Evaluation of widely used diagnostic tests to detect West Nile virus infections in horses previously infected with St. Louis encephalitis virus or dengue virus type 2","volume":"18","author":"Ledermann","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1128\/CVI.00393-08","article-title":"Evaluation of an epitope-blocking enzyme-linked immunosorbent assay for the diagnosis of West Nile virus infections in humans","volume":"16","author":"Blitvich","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1002\/jmv.22180","article-title":"Acute West Nile virus neuroinvasive infections: cross-reactivity with dengue virus and tick-borne encephalitis virus","volume":"83","author":"Papa","year":"2011","journal-title":"J. Med. Virol."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1292\/jvms.71.969","article-title":"Antibody responses induced by experimental West Nile Virus infection with or without previous immunization with inactivated Japanese encephalitis vaccine in horses","volume":"71","author":"Shirafuji","year":"2009","journal-title":"J. Vet. Med. Sci."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1292\/jvms.09-0311","article-title":"Cross-reactivity of Japanese encephalitis virus-vaccinated horse sera in serodiagnosis of West Nile virus","volume":"72","author":"Hirota","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1089\/vbz.2011.0709","article-title":"A diagnostic algorithm to serologically differentiate West Nile Virus from Japanese Encephalitis Virus infections and its validation in field surveillance of poultry and horses","volume":"12","author":"Yeh","year":"2012","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4269\/ajtmh.2001.65.379","article-title":"Experimental infections of pigs with Japanese encephalitis virus and closely related Australian flaviviruses","volume":"65","author":"Williams","year":"2001","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_156","doi-asserted-by":"crossref","unstructured":"Buckley, A., Dawson, A., and Gould, E.A. (2006). Detection of seroconversion to West Nile virus, Usutu virus and Sindbis virus in UK sentinel chickens. Virol. J., 3.","DOI":"10.1186\/1743-422X-3-71"},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"19812:1","DOI":"10.2807\/ese.16.10.19812-en","article-title":"Increasing West Nile virus antibody titres in central European plasma donors from 2006 to 2010","volume":"16","author":"Rabel","year":"2011","journal-title":"Euro. Surveill."},{"key":"ref_158","doi-asserted-by":"crossref","unstructured":"Sonnleitner, S.T., Simeoni, J., Baumgartner, R., Zelger, R., Prader, A., Piccolini, G., Nowotny, N., and Walder, G. (2013). The spreading of flaviviruses over the continental divide: A challenge for serologic diagnostics. J. Med. Microbiol. Diagn., 3.","DOI":"10.4172\/2161-0703.S3-002"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1089\/vbz.2006.0592","article-title":"West Nile virus in wild resident birds, Southern France, 2004","volume":"7","author":"Jourdain","year":"2007","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_160","doi-asserted-by":"crossref","unstructured":"Alba, A., Allepuz, A., Napp, S., Soler, M., Selga, I., Aranda, C., Casal, J., Pages, N., Hayes, E.B., and Busquets, N. (2013). Ecological Surveillance for West Nile in Catalonia (Spain), Learning from a Five-Year Period of Follow-up. Zoonoses Public Health, 2013.","DOI":"10.1111\/zph.12048"},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1089\/vbz.2007.0200","article-title":"Prevalence of West Nile virus neutralizing antibodies in Spain is related to the behavior of migratory birds","volume":"8","author":"Lopez","year":"2008","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.3201\/eid1312.070343","article-title":"Seroconversion in wild birds and local circulation of west nile virus, Spain","volume":"13","author":"Figuerola","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1089\/vbz.2009.0028","article-title":"Surveillance for West Nile virus in wild birds from Northern Europe","volume":"11","author":"Jourdain","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_164","doi-asserted-by":"crossref","unstructured":"Valiakos, G., Touloudi, A., Athanasiou, L.V., Giannakopoulos, A., Iacovakis, C., Birtsas, P., Spyrou, V., Dalabiras, Z., Petrovska, L., and Billinis, C. (2012). Serological and molecular investigation into the role of wild birds in the epidemiology of West Nile virus in Greece. Virol. J., 9.","DOI":"10.1186\/1743-422X-9-266"},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1089\/vbz.2011.0843","article-title":"Do wild ungulates allow improved monitoring of flavivirus circulation in Spain?","volume":"12","author":"Boadella","year":"2012","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jevs.2007.11.009","article-title":"West Nile Virus antibody titers and total immunoglobulin G concentrations in Foals from Mares Vaccinated in Late Gestation","volume":"28","author":"Turner","year":"2008","journal-title":"J. Equine Vet. Sci."},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4269\/ajtmh.2008.79.283","article-title":"Passive immunity to West Nile virus provides limited protection in a common passerine species","volume":"79","author":"Nemeth","year":"2008","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"310","DOI":"10.4269\/ajtmh.2007.76.310","article-title":"Dynamics of passive immunity to West Nile virus in domestic chickens (Gallus gallus domesticus)","volume":"76","author":"Nemeth","year":"2007","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1017\/S0950268811000173","article-title":"Spread of West Nile virus in Iran: A cross-sectional serosurvey in equines, 2008\u20132009","volume":"139","author":"Ahmadnejad","year":"2011","journal-title":"Epidemiol. Infect."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"480","DOI":"10.4269\/ajtmh.2006.75.480","article-title":"Previous infection with West Nile or St. Louis encephalitis viruses provides cross protection during reinfection in house finches","volume":"75","author":"Fang","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1637\/8574-010109-Reg.1","article-title":"Cross-protection between West Nile and Japanese encephalitis viruses in red-winged blackbirds (Agelaius phoeniceus)","volume":"53","author":"Nemeth","year":"2009","journal-title":"Avian Dis."},{"key":"ref_172","first-page":"277","article-title":"Two-way cross-protection between West Nile and Japanese encephalitis viruses in bonnet macaques","volume":"36","author":"Goverdhan","year":"1992","journal-title":"Acta. Virol."},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3201\/eid0803.010238","article-title":"Immunization with heterologous flaviviruses protective against fatal West Nile encephalitis","volume":"8","author":"Tesh","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"ref_174","first-page":"611","article-title":"The mechanism of cross-protection afforded by dengue virus against West Nile virus in hamsters","volume":"70","author":"Price","year":"1972","journal-title":"J. Hyg."},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1128\/iai.9.5.909-915.1974","article-title":"Cross-protection between group B arboviruses: Resistance in mice to Japanese B encephalitis and St. Louis encephalitis viruses induced by dengue virus immunization","volume":"9","author":"Tarr","year":"1974","journal-title":"Infect. Immun."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"4785","DOI":"10.1016\/j.vaccine.2005.04.036","article-title":"The suitability of yellow fever and Japanese encephalitis vaccines for immunization against West Nile virus","volume":"23","author":"Yamshchikov","year":"2005","journal-title":"Vaccine"},{"key":"ref_177","doi-asserted-by":"crossref","first-page":"10324","DOI":"10.1128\/JVI.00480-13","article-title":"An inactivated cell-culture Japanese encephalitis vaccine (JE-ADVAX) formulated with delta inulin adjuvant provides robust heterologous protection against West Nile encephalitis via cross-protective memory B cells and neutralizing antibody","volume":"87","author":"Petrovsky","year":"2013","journal-title":"J. Virol."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1128\/JVI.02273-08","article-title":"Live chimeric and inactivated Japanese encephalitis virus vaccines differ in their cross-protective values against Murray Valley encephalitis virus","volume":"83","author":"Lobigs","year":"2009","journal-title":"J. Virol."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1093\/infdis\/jir122","article-title":"A tick-borne encephalitis virus vaccine based on the European prototype strain induces broadly reactive cross-neutralizing antibodies in humans","volume":"203","author":"Orlinger","year":"2011","journal-title":"J. Infect. Dis."},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"12497","DOI":"10.1128\/JVI.78.22.12497-12507.2004","article-title":"ChimeriVax-West Nile virus live-attenuated vaccine: Preclinical evaluation of safety, immunogenicity, and efficacy","volume":"78","author":"Arroyo","year":"2004","journal-title":"J. Virol."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1017\/S0022172400070923","article-title":"Immunological enhancement and the pathogenesis of dengue haemorrhagic fever","volume":"89","author":"Porterfield","year":"1982","journal-title":"J. Hyg."},{"key":"ref_182","first-page":"301","article-title":"Immune enhancement of viral infection","volume":"31","author":"Halstead","year":"1982","journal-title":"Prog. Allergy"},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.3390\/v2122649","article-title":"The complexity of antibody-dependent enhancement of dengue virus infection","volume":"2","author":"Guzman","year":"2010","journal-title":"Viruses"},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1099\/0022-1317-66-8-1831","article-title":"Antibody-dependent enhancement of tick-borne encephalitis virus infectivity","volume":"66","author":"Phillpotts","year":"1985","journal-title":"J. Gen. Virol."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1080\/00365540801995386","article-title":"Severe tick-borne encephalitis in a patient previously infected by West Nile virus","volume":"40","author":"Ferenczi","year":"2008","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_186","doi-asserted-by":"crossref","unstructured":"Vittecoq, M., Thomas, F., Jourdain, E., Moutou, F., Renaud, F., and Gauthier-Clerc, M. (2012). Risks of emerging infectious diseases: Evolving threats in a changing area, the mediterranean basin. Transbound. Emerg. Dis., 2012.","DOI":"10.1111\/tbed.12012"},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.prevetmed.2012.01.009","article-title":"The consequences of risk-based surveillance: Developing output-based standards for surveillance to demonstrate freedom from disease","volume":"105","author":"Cameron","year":"2012","journal-title":"Prev. Vet. Med."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.prevetmed.2011.12.014","article-title":"Policy-driven development of cost-effective, risk-based surveillance strategies","volume":"105","author":"Reist","year":"2012","journal-title":"Prev. Vet. Med."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/11\/6049\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:50:33Z","timestamp":1760219433000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/10\/11\/6049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,12]]},"references-count":188,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2013,11]]}},"alternative-id":["ijerph10116049"],"URL":"https:\/\/doi.org\/10.3390\/ijerph10116049","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,12]]}}}