{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:29:55Z","timestamp":1768400995168,"version":"3.49.0"},"reference-count":74,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T00:00:00Z","timestamp":1387238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Two hundred and forty avian broilers were equally divided into four groups, and raised with a corn-soybean basal diet or the same diet supplemented with 300, 600, 900 mg\/kg NiCl2 for 42 days. Numbers or percentages of apoptotic splenocytes by flow cytometry (FCM) and TUNEL were higher (p &lt; 0.05 or p &lt; 0.01) in the 300, 600 and 900 mg\/kg groups than those in the control group. Results measured by qRT-PCR and ELISA showed that mRNA expression and contents were significantly higher (p &lt; 0.05 or p &lt; 0.01) in Bax and Caspase-3, and were significantly lower (p &lt; 0.05 or p &lt; 0.01) in Bcl-2 of the 300, 600 and 900 mg\/kg groups. Also, the SOD, CAT and GSH-Px activities, and the ability to inhibit hydroxyl radical, and GSH contents were significantly decreased (p &lt; 0.05 or p &lt; 0.01), and MDA contents were increased (p &lt; 0.05 or p &lt; 0.01) in all groups. In conclusion, dietary NiCl2 in excess of 300 mg\/kg caused apoptosis, altered Bax, Bcl-2 and Caspase-3 mRNA expression levels and contents, and induced oxidative stress in the spleen. Also, splenocyte apoptosis was closely related to the alternations of Bax, Bcl-2 and Caspase-3 mRNA expression, and oxidative damage. The splenic immunity and blood filtration functions were impaired in broilers.<\/jats:p>","DOI":"10.3390\/ijerph10127310","type":"journal-article","created":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T12:13:31Z","timestamp":1387282411000},"page":"7310-7326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["The Association between Splenocyte Apoptosis and Alterations of Bax, Bcl-2 and Caspase-3 mRNA Expression, and Oxidative Stress Induced by Dietary Nickel Chloride in Broilers"],"prefix":"10.3390","volume":"10","author":[{"given":"Jianying","family":"Huang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2963-531X","authenticated-orcid":false,"given":"Hengmin","family":"Cui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Peng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhicai","family":"Zuo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junliang","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bangyuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2013,12,17]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Toxicity of nickel to soil organisms in Denmark","volume":"148","year":"1997","journal-title":"Rev. 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