{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:37:45Z","timestamp":1792085865881,"version":"4.4.0"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:00:00Z","timestamp":1399507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Detection of leptospires based on DNA amplification techniques is essential for the early diagnosis of leptospirosis when anti-Leptospira antibodies are below the detection limit of most serological tests. In middle and low income countries where leptospirosis is endemic, routine implementation of real-time PCR is financially and technically challenging due to the requirement of expensive thermocycler equipment. In this study we report the development and evaluation of a novel isothermal recombinase polymerase amplification assay (RPA) for detection of pathogenic Leptospira based on TwistAmp chemistry. RPA enabled the detection of less than two genome copies per reaction. Retrospective evaluation revealed a high diagnostic accuracy (sensitivity and specificity  of 94.7% and 97.7%, respectively) compared to culturing as the reference standard.  RPA presents a powerful tool for the early diagnosis of leptospirosis in humans and in animals. Furthermore, it enables the detection of the causative agent in reservoirs and environment, and as such is a valuable adjunct to current tools for surveillance and early outbreak warning.<\/jats:p>","DOI":"10.3390\/ijerph110504953","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T11:45:35Z","timestamp":1399549535000},"page":"4953-4964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira"],"prefix":"10.3390","volume":"11","author":[{"given":"Ahmed","family":"Ahmed","sequence":"first","affiliation":[{"name":"KIT Biomedical Research, WHO\/FAO\/OIE and National Leptospirosis Reference Centre,  Royal Tropical Institute, Meibergdreef 39, Amsterdam 1105, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Van der Linden","sequence":"additional","affiliation":[{"name":"KIT Biomedical Research, WHO\/FAO\/OIE and National Leptospirosis Reference Centre,  Royal Tropical Institute, Meibergdreef 39, Amsterdam 1105, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudy","family":"Hartskeerl","sequence":"additional","affiliation":[{"name":"KIT Biomedical Research, WHO\/FAO\/OIE and National Leptospirosis Reference Centre,  Royal Tropical Institute, Meibergdreef 39, Amsterdam 1105, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2014,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2013.09.012","article-title":"Rapid tests for diagnosis of leptospirosis: Current tools and emerging technologies","volume":"78","author":"Picardeau","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ahmed, A., Engelberts, M.F., Boer, K.R., Ahmed, N., and Hartskeerl, R.A. (2009). Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials. PLoS One, 4.","DOI":"10.1371\/journal.pone.0007093"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","first-page":"436","article-title":"Detectie van pathogene leptospiren met behulp van NASBA","volume":"138","author":"Colebrander","year":"1994","journal-title":"Ned Tijdschr Geneeskd."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1128\/JCM.00481-12","article-title":"A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine","volume":"50","author":"Koizumi","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.diagmicrobio.2008.10.012","article-title":"Application of a loop-mediated isothermal amplification method for the detection of pathogenic Leptospira","volume":"63","author":"Lin","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"614","DOI":"10.4269\/ajtmh.2011.10-0473","article-title":"Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand","volume":"84","author":"Sonthayanon","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.mimet.2012.08.008","article-title":"Development of a novel primer combination to detect pathogenic Leptospira by loop-mediated isothermal amplification","volume":"91","author":"Suwancharoen","year":"2012","journal-title":"J. Microbiol. Methods"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ahmed, A., Grobusch, M.P., Klatser, P.R., and Hartskeerl, R.A. (2012). Molecular approaches in the detection and characterization of Leptospira. J. Bacteriol. Parasitol., 3.","DOI":"10.4172\/2155-9597.1000133"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Piepenburg, O., Williams, C.H., Stemple, D.L., and Armes, N.A. (2006). DNA detection using recombination proteins. PLoS Biol., 4.","DOI":"10.1371\/journal.pbio.0040204"},{"key":"ref_11","unstructured":"Van Steenbergen, J.E., Timen, A., and Beaujean, D.J.M.A. (2008). LCI-richtlijnen Infectieziektebestrijding, Landelijke Coordinatie Infectieziektebestrijding."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1099\/00207713-49-2-839","article-title":"Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies","volume":"49","author":"Brenner","year":"1999","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1099\/ijs.0.63783-0","article-title":"Leptospira broomii sp. nov., isolated from humans with leptospirosis","volume":"56","author":"Levett","year":"2006","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Matthias, M.A., Ricaldi, J.N., Cespedes, M., Diaz, M.M., Galloway, R.L., Saito, M., Steigerwalt, A.G., Patra, K.P., Ore, C.V., and Gotuzzo, E. (2008). Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Negl. Trop. Dis., 2.","DOI":"10.1371\/journal.pntd.0000213"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1099\/00207713-48-3-851","article-title":"Leptospira fainei sp. nov. isolated from pigs in Australia","volume":"48","author":"Perolat","year":"1998","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Victoria, B., Ahmed, A., Zuerner, R.L., Ahmed, N., Bulach, D.M., Quinteiro, J., and Hartskeerl, R.A. (2008). Conservation of the S10-spc-\u03b1 locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira. PLoS One, 3.","DOI":"10.1371\/journal.pone.0002752"},{"key":"ref_17","unstructured":"Goris, M.G.A., Leeflang, M.M., Boer, K.R., Goeijenbier, M., van Gorp, E.C.M., Wagenaar, J.F., and Hartskeerl, R.A. (2012). Establishment of valid laboratory case definition for human leptospirosis. J. Bacteriol. Parasitol., 3."},{"key":"ref_18","first-page":"45","article-title":"Nutrition of Leptospira Pomona and growth of 13 other serotypes: Fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80","volume":"26","author":"Ellinghausen","year":"1965","journal-title":"Am. J. Vet. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/jb.94.1.27-31.1967","article-title":"Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures","volume":"94","author":"Johnson","year":"1967","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ahmed, A., Engelberts, M.F., Boer, K.R., Ahmed, N., and Hartskeerl, R.A. (2009). Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials. PLoS One, 4.","DOI":"10.1371\/journal.pone.0007093"},{"key":"ref_21","unstructured":"BLAST: Basic Local Alignment Search Tool. Available online:http:\/\/www.blast.ncbi.nlm.nih.gov\/Blast.cgi."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the lipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E","article-title":"Two-sided confidence intervals for the single proportion: Comparison of seven methods","volume":"17","author":"Newcombe","year":"1998","journal-title":"Stat. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1080\/01621459.1927.10502953","article-title":"Probable inference, the law of succession, and statistical inference","volume":"22","author":"Wilson","year":"1927","journal-title":"J. Am. Stat. Assoc."},{"key":"ref_25","unstructured":"VassarStats: Website for Statistical Computation. Available online:http:\/\/www.vassarstats.net."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1128\/IAI.68.4.2276-2285.2000","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection","volume":"68","author":"Haake","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1099\/00221287-139-8-1691","article-title":"Detection of seven species of pathogenic leptospires by PCR using two sets of primers","volume":"139","author":"Gravekamp","year":"1993","journal-title":"J. Gen. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1099\/00221287-139-8-1681","article-title":"Characterization of Leptospiraceae by 16S DNA restriction fragment length polymorphisms","volume":"139","author":"Hookey","year":"1993","journal-title":"J. Gen. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1099\/00221287-139-11-2585","article-title":"The use of 16S rDNA sequence analysis to investigate the phylogeny of Leaptospirceae and related spirochaetes","volume":"139","author":"Hookey","year":"1993","journal-title":"J. Gen. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1128\/jcm.35.1.248-253.1997","article-title":"Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L. borgpetersenii, and L. kirschneri) with species-specific DNA probes produced by arbitrarily primed PCR","volume":"35","author":"Letocart","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1574-6968.1997.tb10262.x","article-title":"Oligonucleotides specific for pathogenic and saprophytic Leptospira occurring in water","volume":"148","author":"Murgia","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1128\/iai.60.4.1707-1710.1992","article-title":"Presence of putative sphingomyelinase genes among members of the family Leptospiraceae","volume":"60","author":"Segers","year":"1992","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.meegid.2010.06.002","article-title":"A simple and rapid molecular method for Leptospira species identification","volume":"10","author":"Ahmed","year":"2010","journal-title":"Infect. Genet. Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1093\/cid\/cis403","article-title":"Fool\u2019s gold: Why imperfect reference tests are undermining the evaluation of novel diagnostics: A reevaluation of 5 diagnostic tests for leptospirosis","volume":"55","author":"Limmathurotsakul","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_35","first-page":"70","article-title":"Viability of Leptospira interrogans serotype Grippotyphosa in swine urine and blood","volume":"68","author":"Nervig","year":"1978","journal-title":"Cornell Vet."},{"key":"ref_36","unstructured":"Word Health Organization (2003). Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control, WHO."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/11\/5\/4953\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:11:10Z","timestamp":1760217070000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/11\/5\/4953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,8]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2014,5]]}},"alternative-id":["ijerph110504953"],"URL":"https:\/\/doi.org\/10.3390\/ijerph110504953","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,5,8]]}}}