{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:10:36Z","timestamp":1791335436103,"version":"4.3.1"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,2]],"date-time":"2016-01-02T00:00:00Z","timestamp":1451692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Maturation ponds are used in rural and regional areas in Australia to remove the microbial loads of sewage wastewater, however, they have not been studied intensively until present. Using a combination of culture-based methods and quantitative real-time PCR, we assessed microbial removal rates in maturation ponds at four waste stabilization ponds (WSP) with (n = 1) and without (n = 3) baffles in rural and remote communities in Australia. Concentrations of total coliforms, E. coli, enterococci, Campylobacter spp., Salmonella spp., F+ RNA coliphage, adenovirus, Cryptosporidium spp. and Giardia (oo) cysts in maturation ponds were measured at the inlet and outlet. Only the baffled pond demonstrated a significant removal of most of the pathogens tested and therefore was subjected to further study by analyzing E. coli and enterococci concentrations at six points along the baffles over five sampling rounds. Using culture-based methods, we found a decrease in the number of E. coli and enterococci from the initial values of 100,000 CFU per 100 mL in the inlet samples to approximately 1000 CFU per 100 mL in the outlet samples for both bacterial groups. Giardia cysts removal was relatively higher than fecal indicators reduction possibly due to sedimentation.<\/jats:p>","DOI":"10.3390\/ijerph13010096","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T02:29:07Z","timestamp":1451960947000},"page":"96","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Removal of Fecal Indicators, Pathogenic Bacteria, Adenovirus, Cryptosporidium and Giardia (oo)cysts in Waste Stabilization Ponds in Northern and Eastern Australia"],"prefix":"10.3390","volume":"13","author":[{"given":"Maxim","family":"Sheludchenko","sequence":"first","affiliation":[{"name":"Smart Water Research Centre, Building G51, Griffith University, Southport, Queensland 4222, Australia"},{"name":"Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Padovan","sequence":"additional","affiliation":[{"name":"Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory 0909, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Katouli","sequence":"additional","affiliation":[{"name":"Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Stratton","sequence":"additional","affiliation":[{"name":"Smart Water Research Centre, Building G51, Griffith University, Southport, Queensland 4222, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.5004\/dwt.2009.359","article-title":"Waste stabilization ponds: Past, present and future","volume":"4","author":"Mara","year":"2009","journal-title":"Desalin. Water Treat."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mara, D. (2006, January 25\u201327). Constructed wetlands are not a viable alternative or addition to waste stabilization ponds. Proceeding of the 7th IWA Specialist Conference On Waste Stabilization Ponds, Bangkok, Thailand.","DOI":"10.1080\/09593332608618690"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.ecoleng.2005.07.008","article-title":"The role of constructed wetlands in secondary effluent treatment and water reuse in subtropical and arid australia","volume":"25","author":"Greenway","year":"2005","journal-title":"Ecol. Eng."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1080\/14486563.2004.10648617","article-title":"Quality criteria in australian reclaimed water guidelines and effluent discharge licences: How consistent are we?","volume":"11","author":"Higgins","year":"2004","journal-title":"Australas. J. Environ. Manag."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4918","DOI":"10.1016\/j.watres.2009.03.041","article-title":"Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR","volume":"43","author":"Ahmed","year":"2009","journal-title":"Water Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"695","DOI":"10.2166\/wh.2011.154","article-title":"Culture-based indicators of fecal contamination and molecular microbial indicators rarely correlate with Campylobacter spp. in recreational waters","volume":"9","author":"Hellein","year":"2011","journal-title":"J. Water Health"},{"key":"ref_7","unstructured":"NRMMC-EPHC-AHMC (2008). Australian Guidelines for Water Recycling: Managing Health and Environmental Risks (Phase 2): Augmentation of Drinking Water Supplies, NRMMC-EPHC-AHMC. Volume Draft for Public Comment."},{"key":"ref_8","unstructured":"APHA (1992). Standard Methods for the Examination of Water and Wastewater, American Public Health Assosiation. [18th ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.1745-4581.2003.tb00031.x","article-title":"Convenient method for rapid and quantitative assessment of Salmonella enterica contamination: The Mini-MSRV MPN technique","volume":"11","author":"Fravalo","year":"2003","journal-title":"J. Rapid Methods Autom. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1128\/AEM.03457-12","article-title":"Mechanisms of human adenovirus inactivation by sunlight and UVC light as examined by quantitative pcr and quantitative proteomics","volume":"79","author":"Bosshard","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_11","unstructured":"IDT PrimerDesign. Available online: http:\/\/www.idtdna.com\/primerquest\/home\/index."},{"key":"ref_12","unstructured":"GenBank BlastN, Available online: http:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1039\/c2em30019b","article-title":"Internally controlled pcr system for detection of airborne microorganisms","volume":"14","author":"Usachev","year":"2012","journal-title":"J. Environ. Monit."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0167-7012(02)00150-1","article-title":"Application of the fluorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples","volume":"52","author":"Frahm","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"ref_15","unstructured":"USEPA (2012). Method A: Enterococci in Water by TaqMan Quantitative Polymerase Chain Reaction (qPCR) Assay, Office of Water."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/jmv.10382","article-title":"Rapid and quantitative detection of human adenovirus DNA by real-time PCR","volume":"70","author":"Heim","year":"2003","journal-title":"J. Med. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1128\/AEM.70.4.2296-2306.2004","article-title":"Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR","volume":"70","author":"Inglis","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","unstructured":"Matthews, B., Stratton, H.M., Schreoder, S., and Toze, S. (2010). Pathogen Detection Technologies for Wastewater and Reservoirs, UWSRA. Urban Water Security Research Alliance Technical Report No. 32."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0928-8244(03)00168-8","article-title":"Application of real-time PCR for quantitative detection of Campylobacter jejuni in poultry, milk and environmental water","volume":"38","author":"Yang","year":"2003","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_20","first-page":"30","article-title":"Novel Salmonella spp. diagnostic markers based on the gyrB gene and its application on food and environmentally-delivered water samples","volume":"1","author":"Leo","year":"2013","journal-title":"J. Adv. Biotechnol. Bioeng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1128\/AEM.03360-13","article-title":"Enumeration of salmonellae in table eggs, pasteurized egg products, and egg-containing dishes by using quantitative real-time PCR","volume":"80","author":"Pasquali","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1046\/j.1472-765X.1999.00658.x","article-title":"Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli","volume":"29","author":"Denis","year":"1999","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"589","DOI":"10.2166\/wst.2012.896","article-title":"Removal improvement of bacteria (Escherichia coli and Enterococci) in maturation ponds using baffles","volume":"65","author":"Ouali","year":"2012","journal-title":"Water Sci. Technol."},{"key":"ref_24","unstructured":"Shilton, A. (2005). Pond Treatment Technology, IWA Publishing."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.envint.2003.11.006","article-title":"Fate and transport of pathogens in lakes and reservoirs","volume":"30","author":"Brooks","year":"2004","journal-title":"Environ. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1021\/es061716i","article-title":"Sunlight-mediated inactivation of MS2 coliphage via exogenous singlet oxygen produced by sensitizers in natural waters","volume":"41","author":"Kohn","year":"2006","journal-title":"Environ. Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1128\/am.27.5.823-829.1974","article-title":"Comparative survival of indicator bacteria and enteric pathogens in well water","volume":"27","author":"McFeters","year":"1974","journal-title":"Appl. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3109\/10408419709115135","article-title":"Natural protection of spring and well drinking water against surface microbial contamination. II. Indicators and monitoring parameters for parasites","volume":"23","author":"Edberg","year":"1997","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/S0043-1354(98)00321-2","article-title":"Inactivation of faecal indicator micro-organisms in waste stabilisation ponds: Interactions of environmental factors with sunlight","volume":"33","author":"Donnison","year":"1999","journal-title":"Water Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.watres.2013.10.046","article-title":"Sunlight mediated inactivation mechanisms of Enterococcus faecalis and Escherichia coli in clear water vs. waste stabilization pond water","volume":"50","author":"Kadir","year":"2014","journal-title":"Water Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.watres.2015.06.043","article-title":"Sunlight inactivation of fecal indicator bacteria in open-water unit process treatment wetlands: Modeling endogenous and exogenous inactivation rates","volume":"83","author":"Nguyen","year":"2015","journal-title":"Water Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.jviromet.2012.11.002","article-title":"Sensitive detection of human adenovirus from small volume of primary wastewater samples by quantitative PCR","volume":"187","author":"Sidhu","year":"2013","journal-title":"J. Virol. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1080\/09603129973227","article-title":"Removal and destruction of intestinal parasitic protozoans by sewage treatment processes","volume":"9","author":"Robertson","year":"1999","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1007\/s00248-010-9791-6","article-title":"Mechanisms for parasites removal in a waste stabilisation pond","volume":"61","author":"Reinoso","year":"2011","journal-title":"Microb. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"357","DOI":"10.2166\/aqua.2006.044","article-title":"The occurrence of Cryptosporidium and Giardia in the Lake Baroon catchment, Queensland, Australia","volume":"55","author":"Wohlsen","year":"2006","journal-title":"J. Water Supply Res. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.watres.2007.06.048","article-title":"Behaviour of pathogenic and indicator bacteria during urban wastewater treatment and sludge composting, as revealed by quantitative PCR","volume":"42","author":"Lhoutellier","year":"2008","journal-title":"Water Res."},{"key":"ref_37","unstructured":"Oliver, J.D. (2000). Problems in Detecting Dormant (VBNC) Cells, and the Role of DNA Elements in This Response, Landes Bioscience."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.mimet.2011.09.007","article-title":"Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration","volume":"87","author":"Trevors","year":"2011","journal-title":"J. Microbiol. Method."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5154","DOI":"10.1128\/AEM.65.11.5154-5157.1999","article-title":"Recovery in embryonated eggs of viable but nonculturable Campylobacter jejuni cells and maintenance of ability to adhere to hela cells after resuscitation","volume":"65","author":"Cappelier","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"93","DOI":"10.2166\/wst.2005.0503","article-title":"Hygienization by anaerobic digestion: Comparison between evaluation by cultivation and quantitative real-time PCR","volume":"52","author":"Lebuhn","year":"2005","journal-title":"Water Sci. Technol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.mcp.2004.09.004","article-title":"Persistence of extracellular baculoviral DNA in aquatic microcosms: Extraction, purification, and amplification by the polymerase chain reaction (PCR)","volume":"19","author":"England","year":"2005","journal-title":"Mol. Cell. Probes"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00216-012-6399-3","article-title":"Application of quantitative PCR for the detection of microorganisms in water","volume":"405","author":"Botes","year":"2013","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1128\/aem.48.5.1026-1030.1984","article-title":"Modified agar medium for detecting environmental salmonellae by the most-probable-number method","volume":"48","author":"Hussong","year":"1984","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_44","first-page":"569","article-title":"Rapid quantification of salmonella in seafood by real-time PCR assay","volume":"20","author":"Kumar","year":"2010","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.scitotenv.2007.02.039","article-title":"Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment","volume":"382","author":"Shannon","year":"2007","journal-title":"Sci. Total Environ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"569","DOI":"10.2166\/wst.1987.0236","article-title":"The removal of excreted bacteria and viruses in deep waste stabilization ponds in northeast Brazil","volume":"19","author":"Oragui","year":"1987","journal-title":"Water Sci. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1128\/AEM.07180-11","article-title":"Improvement of modified charcoal-cefoperazone-deoxycholate agar by supplementation with a high concentration of polymyxin B for detection of Campylobacter jejuni and C. coli in chicken carcass rinses","volume":"78","author":"Chon","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/JCM.38.1.286-291.2000","article-title":"Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for Campylobacters","volume":"38","author":"Engberg","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3378","DOI":"10.1016\/j.watres.2011.03.050","article-title":"Evaluation of low-copy genetic targets for waterborne bacterial pathogen detection via qPCR","volume":"45","author":"Clark","year":"2011","journal-title":"Water Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.mimet.2007.02.016","article-title":"Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds","volume":"69","author":"Khan","year":"2007","journal-title":"J. Microbiol. Method."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/13\/1\/96\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:17:08Z","timestamp":1760210228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/13\/1\/96"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,2]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,1]]}},"alternative-id":["ijerph13010096"],"URL":"https:\/\/doi.org\/10.3390\/ijerph13010096","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,2]]}}}