{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:16:59Z","timestamp":1791839819114,"version":"4.3.4"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T00:00:00Z","timestamp":1503532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>This study seeks to investigate the possible protective role of the methanol extract of Piper guineense seeds against CCl4-induced hepatotoxicity in an animal model. Hepatotoxicity was induced by administering oral doses of CCl4 (1.2 g\/kg bw) three times a week for three weeks. Group 1 (Control) and Group 2 (CCl4) were left untreated; Piper guineense (PG; 400 mg\/kg bw) was administered to Group 3 (T1) by oral gavage for 14 days prior to the administration of CCl4 and simultaneously with CCl4; PG (400 mg\/kg bw) was administered simultaneously with CCl4 in Group 4 (T2); and Livolin forte (20 mg\/kg bw) was administered simultaneously with CCl4 in Group 5 (T3), the standard drug group. The administration of CCl4 induces histopathological alteration in the liver, with concomitant increased activities of serum hepatic marker enzymes associated with increased levels of lipid peroxidation. Similarly, there was decrease in non-enzymatic (reduced glutathione) and enzymatic antioxidants (glutathione S-transferase), superoxide dismutase, and catalase. An elevation in serum triglyceride and total cholesterol levels was noticed along with decreased levels of serum total protein. Treatment with PG 400 mg\/kg bw exhibited excellent modulatory activity with respect to the different parameters studied by reversing all the above-mentioned biochemical changes significantly in the experimental animals. These results suggest that PG offered protection comparable to that of Livolin forte with better efficacy when pre-treated with 400 mg\/kg bw 14 days prior to CCl4-exposure.<\/jats:p>","DOI":"10.3390\/ijerph14090955","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T08:44:54Z","timestamp":1503564294000},"page":"955","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Modulatory Effect of Methanol Extract of Piper guineense in CCl4-Induced Hepatotoxicity in Male Rats"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2165-7936","authenticated-orcid":false,"given":"Babatunji","family":"Oyinloye","sequence":"first","affiliation":[{"name":"Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology University of Zululand, KwaDlangezwa 3886, South Africa"},{"name":"Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti 360001, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foluso","family":"Osunsanmi","sequence":"additional","affiliation":[{"name":"Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology University of Zululand, KwaDlangezwa 3886, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basiru","family":"Ajiboye","sequence":"additional","affiliation":[{"name":"Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti 360001, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9331-396X","authenticated-orcid":false,"given":"Oluwafemi","family":"Ojo","sequence":"additional","affiliation":[{"name":"Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, PMB 5454, Ado-Ekiti 360001, Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2521-8957","authenticated-orcid":false,"given":"Abidemi","family":"Kappo","sequence":"additional","affiliation":[{"name":"Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology University of Zululand, KwaDlangezwa 3886, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1002\/hep.21347","article-title":"Global challenges in liver disease","volume":"44","author":"Williams","year":"2006","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S350","DOI":"10.2105\/AJPH.2013.301645","article-title":"Mortality caused by chronic liver disease among American Indians and Alaska Natives in the United States, 1999\u20132009","volume":"104","author":"Suryaprasad","year":"2014","journal-title":"Am. 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