{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:11:16Z","timestamp":1793344276894,"version":"4.5.0"},"reference-count":21,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T00:00:00Z","timestamp":1511222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Seroprevalence of Leptospira spp. in cattle is unknown in Uganda. The aim of this study was to estimate the seroprevalence of L. interrogans Icterohaemorrhagiae, Pomona, L. kirschneri Butembo, Grippotyphosa, L. borgpetersenii Nigeria, Hardjo, Wolfii, and Kenya and an overall seroprevalence in cattle from Kole and Mbale districts. Two hundred-seventy five bovine sera from 130 small holder farms from Kole (n = 159) and Mbale (n = 116), collected between January and July 2015, were tested for antibodies against eight Leptospira strains by Microscopic Agglutination Test. A titer of \u2265100 was considered seropositive, indicating past exposure. Overall, the seroprevalence was 19.27% (95% CI 14.9\u201324.5%). Pomona seroprevalence was highest with 9.45% (6.4\u201313.7%), followed by Kenya 5.09% (2.9\u20138.6%), Nigeria 4.00% (2.1\u20137.2%), Wolfii 3.27% (1.6\u20136.3%), Butembo 1.86% (0.7\u20134.4%), Hardjo 1.45% (0.5\u20133.9%), and Icterohaemorragiae and Grippotyphosa with less than 1% positive. Seroprevalence did not differ between districts and gender (p \u2265 0.05). Seven animals had titers \u2265400. Cross-reactions or exposure to \u22651 serovar was measured in 43% of serum samples. Seroprevalence of 19% implies exposure of cattle to leptospires.<\/jats:p>","DOI":"10.3390\/ijerph14111421","type":"journal-article","created":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T11:23:20Z","timestamp":1511263400000},"page":"1421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cross-Sectional Serological Survey for Leptospira spp. in Beef and Dairy Cattle in Two Districts in Uganda"],"prefix":"10.3390","volume":"14","author":[{"given":"Anou","family":"Dreyfus","sequence":"first","affiliation":[{"name":"Section of Epidemiology, Vetsuisse Faculty, University of Zurich, 8006 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9730-4112","authenticated-orcid":false,"given":"Terence","family":"Odoch","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources &amp; Biosecurity, Makerere University, P.O. Box 7062 Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lordrick","family":"Alinaitwe","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources &amp; Biosecurity, Makerere University, P.O. Box 7062 Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1159-484X","authenticated-orcid":false,"given":"Sabrina","family":"Rodriguez-Campos","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2235-7786","authenticated-orcid":false,"given":"Amanuel","family":"Tsegay","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources &amp; Biosecurity, Makerere University, P.O. Box 7062 Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentine","family":"Jaquier","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clovice","family":"Kankya","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Animal Resources &amp; Biosecurity, Makerere University, P.O. Box 7062 Kampala, Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/j.1469-0691.2011.03474.x","article-title":"Emergence, control and re-emerging leptospirosis: Dynamics of infection in the changing world","volume":"17","author":"Hartskeerl","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_3","unstructured":"Faine, S., Adler, B., Bolin, C., and Perolat, P. (1999). Leptospira and Leptospirosis, MediSci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Costa, F., Hagan, J.E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M.S., Stein, C., Abela-Ridder, B., and Ko, A.I. (2015). Global morbidity and mortality of leptospirosis: A systematic review. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003898"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.20506\/rst.9.4.534","article-title":"A new leptospiral serovar in the pyrogenes serogroup isolated in nigeria","volume":"9","author":"Ezeh","year":"1990","journal-title":"Rev. Sci. Tech."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Dreyfus, A., Dyal, J.W., Pearson, R., Kankya, C., Kajura, C., Alinaitwe, L., Kakooza, S., Pelican, K.M., Travis, D.A., and Mahero, M. (2016). Leptospira seroprevalence and risk factors in health centre patients in hoima district, western uganda. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004858"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"680","DOI":"10.3201\/eid1904.121143","article-title":"Serosurvey of dogs for human, livestock, and wildlife pathogens, uganda","volume":"19","author":"Millan","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"288","DOI":"10.4269\/ajtmh.13-0466","article-title":"Seroprevalence of leptospira hardjo in cattle and african buffalos in southwestern uganda","volume":"90","author":"Atherstone","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Allan, K.J., Biggs, H.M., Halliday, J.E.B., Kazwala, R.R., Maro, V.P., Cleaveland, S., and Crump, J.A. (2015). Epidemiology of leptospirosis in africa: A systematic review of a neglected zoonosis and a paradigm for \u2018one health\u2019 in africa. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003899"},{"key":"ref_10","unstructured":"Uganda Bureau of Statistics (UBOS) (2017). The National Population and Housing Census 2014\u2014Area Specific Profile Series, UBOS."},{"key":"ref_11","unstructured":"UBOS, MAAIF Uganda Bureau of Statistics, and Ministry of Agriculture Animal Industries and Fisheries (2009). A Summary Report of the National Livestock Census, 2008."},{"key":"ref_12","first-page":"2224","article-title":"Perceived and actual rainfall trends and variability in eastern uganda: Implications for community preparedness and response","volume":"8","author":"Kansiime","year":"2013","journal-title":"J. Natl. Sci. Res."},{"key":"ref_13","unstructured":"Uganda National Meteorological Authority (2017). Kole Rainfall Data 2015, UNMA."},{"key":"ref_14","unstructured":"Office International des Epizooties (OIE) (2014). Leptospirosis. Oie Terrestrial Manual, OIE."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Goris, M.G., and Hartskeerl, R.A. (2014). Leptospirosis serodiagnosis by the microscopic agglutination test. Curr. Protoc. Microbiol., 32.","DOI":"10.1002\/9780471729259.mc12e05s32"},{"key":"ref_16","unstructured":"Rodriguez, S. (2015). Personal Communication, Pirbright Institute."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_18","unstructured":"Fleiss, J.L. (1981). Statistical Methods for Rates and Proportions, John Wiley & Sons Inc."},{"key":"ref_19","unstructured":"Dreyfus, A. (2013). Leptospirosis in humans and pastoral livestock in new zealand. [Ph.D. Thesis, Massey University]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1017\/S0950268814002477","article-title":"Risk of infection and associated influenza-like disease among abattoir workers due to two leptospira species","volume":"143","author":"Dreyfus","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"ref_21","unstructured":"Goris, M. (2015). Diagnostic tests for human leptospirosis. Second Meeting of the European Leptospirosis Society on Leptospirosis and Other Rodent Borne Hemorrhagic Fevers, European Leptospirosis Society Royal Tropical Institute."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/14\/11\/1421\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:50:29Z","timestamp":1760208629000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/14\/11\/1421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,21]]},"references-count":21,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11]]}},"alternative-id":["ijerph14111421"],"URL":"https:\/\/doi.org\/10.3390\/ijerph14111421","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,21]]}}}