{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:19:00Z","timestamp":1762431540975,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2018,8,3]],"date-time":"2018-08-03T00:00:00Z","timestamp":1533254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"he Major Program of the National Natural Science Foundation of China","award":["21590813"],"award-info":[{"award-number":["21590813"]}]},{"name":"the National Key Research and Development Program","award":["2016YFC0401108"],"award-info":[{"award-number":["2016YFC0401108"]}]},{"name":"the Programme of Introducing Talents of Discipline to Universities","award":["B13012"],"award-info":[{"award-number":["B13012"]}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["DUT16LK17"],"award-info":[{"award-number":["DUT16LK17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>The present study aims to evaluate the potential of transcriptomic profiles in evaluating the impacts of complex mixtures of pollutants at environmentally relevant concentrations on aquatic vertebrates. The changes in gene expression were determined using microarray in the liver of male zebrafish (Danio rerio) exposed to surface water collected from selected locations on the Hun River, China. The numbers of differentially expressed genes (DEGs) in each treatment ranged from 728 to 3292, which were positively correlated with chemical oxygen demand (COD). Predominant transcriptomic responses included peroxisome proliferator-activated receptors (PPAR) signaling and steroid biosynthesis. Key pathways in immune system were also affected. Notably, two human diseases related pathways, insulin resistance and Salmonella infection were enriched. Clustering analysis and principle component analysis with DEGs differentiated the upstream and downstream site of Shenyang City, and the mainstream and the tributary sites near the junction. Comparison the gene expression profiles of zebrafish exposed to river surface water with those to individual chemicals found higher similarity of the river water with estradiol than several other organic pollutants and metals. Results suggested that the transcriptomic profiles of zebrafish is promising in differentiating surface water with pollution gradient and different discharges and in providing valuable information to support discharge management.<\/jats:p>","DOI":"10.3390\/ijerph15081648","type":"journal-article","created":{"date-parts":[[2018,8,3]],"date-time":"2018-08-03T11:03:26Z","timestamp":1533294206000},"page":"1648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Transcriptomic Profiles in Zebrafish Liver Permit the Discrimination of Surface Water with Pollution Gradient and Different Discharges"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhou","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8920-1172","authenticated-orcid":false,"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Qu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xie","family":"Quan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zeng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengchang","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanmei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, Beijing Jiaotong University, Beijing 100044, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixia","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11737","DOI":"10.1021\/es503425w","article-title":"Environmental designer drugs: When transformation may not eliminate risk","volume":"48","author":"Cwiertny","year":"2014","journal-title":"Environ. 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