{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:08:00Z","timestamp":1781845680473,"version":"3.54.5"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T00:00:00Z","timestamp":1539907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Zhejiang Natural Science Foundation","award":["LY17D050006"],"award-info":[{"award-number":["LY17D050006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJERPH"],"abstract":"<jats:p>Airborne bacteria are significantly affected by meteorological and environmental conditions. However, there is little quantitative data available on the effects of these factors on airborne bacteria in urban ecosystems. In the present study, we analyzed weather-dependent changes in the composition of airborne bacterial communities using high throughput sequencing. Samples were collected before and after a period of constant hot weather at four selected sampling sites (YRBS, ZJGUSJC, TJCR, and BLQG) in Hangzhou. Our results show that the average amount of bacterial 16S rRNA gene copy numbers per m3 of air decreased significantly after constant high temperature. In addition, the number of operational taxonomic units and the Shannon\u2013Wiener diversity indexes of the samples at all four selected sampling sites were significantly decreased after the heat event, showing notable impact on bacterial diversity. We also detected a significant increase in the abundances of spore-forming bacteria. Firmicutes increased from 3.7% to 9.9%, Bacillales increased from 2.6% to 7.6%, and Bacillaceae increased from 1.5% to 5.9%. In addition, we observed an increase in beta-Proteobacteria (18.2% to 50.3%), Rhodocyclaceae (6.9% to 29.9%), and Burkholderiaceae (8.1% to 15.2%). On the other hand, the abundance of alpha-Proteobacteria (39.6% to 9.8%), Caulobacteraceae (17.9% to 0.5%), Sphingomonadaceae (7.2% to 3.3%), and Xanthomonadaceae (3.0% to 0.5%) was significantly lower. Taken together, our data suggest that the composition of airborne bacterial communities varies greatly dependent on heat events, and that such communities include several species that are highly susceptible to high-temperature related stressors such as high air temperature, low relative humidity, and high intensity of solar radiation.<\/jats:p>","DOI":"10.3390\/ijerph15102295","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T10:08:02Z","timestamp":1539943682000},"page":"2295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Influence of Heat Events on the Composition of Airborne Bacterial Communities in Urban Ecosystems"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhiguo","family":"Fang","sequence":"first","affiliation":[{"name":"School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijun","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuqin","family":"Lou","sequence":"additional","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1126\/science.1106335","article-title":"Abundance of cellular material and proteins in the atmosphere","volume":"308","author":"Jaenicke","year":"2005","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1016\/j.atmosenv.2006.02.029","article-title":"Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review","volume":"40","author":"Peccia","year":"2006","journal-title":"Atmos. Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.scitotenv.2003.11.021","article-title":"The effects of meteorological factors on atmospheric bioaerosol concentrations\u2014A review","volume":"326","author":"Jones","year":"2004","journal-title":"Sci. Total Environ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"301","DOI":"10.5194\/bg-8-301-2011","article-title":"Detection and phylogenetic analysis of coastal bioaerosols using culture dependent and independent techniques","volume":"7","author":"Urbano","year":"2011","journal-title":"Biogeosciences"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10926","DOI":"10.1021\/es301870t","article-title":"Local environmental pollution strongly influences culturable bacterial aerosols at an urban aquatic superfund site","volume":"46","author":"Dueker","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.scitotenv.2014.01.092","article-title":"Aeration remediation of a polluted waterway increases near-surface coarse and culturable microbial aerosols","volume":"478","author":"Dueker","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1046\/j.1365-2672.2000.01024.x","article-title":"Evaluation of the environmental impact of microbial aerosols generated by wastewater treatment plants utilizing different aeration systems","volume":"88","author":"Brandi","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1472-765X.1995.tb01042.x","article-title":"Detection of coliphages and enteroviruses in sewage and aerosol from an activated sludge wastewater treatment plant","volume":"21","author":"Carducci","year":"2010","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_9","first-page":"81","article-title":"Detection of airborne microbes in a composting facility by cultivation based and cultivation independent methods","volume":"14","author":"Albrecht","year":"2007","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.scitotenv.2014.04.065","article-title":"Characteristics of airborne bacteria in Mumbai urban environment","volume":"488\u2013489","author":"Gangamma","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.atmosenv.2016.05.038","article-title":"Airborne bacteria in the atmosphere: Presence, purpose, and potential","volume":"139","author":"Smets","year":"2016","journal-title":"Atmos. Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/00173130410000794","article-title":"Exposure assessment and analysis for biological agents","volume":"43","author":"Martinez","year":"2004","journal-title":"Grana"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10661-016-5751-z","article-title":"Assessment of multi-contaminant exposure in a cancer treatment center: A 2-year monitoring of molds, mycotoxins, endotoxins, and glucans in bioaerosols","volume":"189","author":"Heutte","year":"2017","journal-title":"Environ. Monit. Assess."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.4209\/aaqr.2014.11.0274","article-title":"Profile and characteristics of culturable bacteria in Hangzhou, southeast of China","volume":"16","author":"Fang","year":"2016","journal-title":"Aerosol Air Qual. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s00248-007-9216-3","article-title":"Culturable airborne bacteria in outdoor environments in Beijing, China","volume":"54","author":"Fang","year":"2007","journal-title":"Microb. Ecol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11783-018-1008-0","article-title":"Effects of heavy rainfall on the composition of airborne bacterial communities","volume":"12","author":"Jang","year":"2018","journal-title":"Front. Environ. Sci. Eng."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6561","DOI":"10.1007\/s00253-012-4450-0","article-title":"Temporal variability and effect of environmental variables on airborne bacterial communities in an urban area of Northern Italy","volume":"97","author":"Bertolini","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"12097","DOI":"10.1021\/es402970s","article-title":"Seasonal variability in bacterial and fungal diversity of the near-surface atmosphere","volume":"47","author":"Bowers","year":"2013","journal-title":"Environ. Sci. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.atmosenv.2013.03.047","article-title":"Temporal variation in airborne microbial populations and microbially-derived allergens in a tropical urban landscape","volume":"74","author":"Woo","year":"2013","journal-title":"Atmos. Environ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.atmosenv.2015.12.008","article-title":"Variation of correlations between factors and culturable airborne bacteria and fungi","volume":"128","author":"Gao","year":"2016","journal-title":"Atmos. Environ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.scitotenv.2014.04.044","article-title":"Variations in the structure of airborne bacterial communities in a down wind area during an Asian dust (Kosa) event","volume":"488","author":"Maki","year":"2014","journal-title":"Sci. Total Environ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1574-6941.1998.tb00515.x","article-title":"Effect of simulated solar radiation on mixed outdoor atmospheric bacterial populations","volume":"26","author":"Tong","year":"1998","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.1016\/j.atmosenv.2010.11.054","article-title":"Impact of Asian dust events on airborne bacterial community assessed by molecular analyses","volume":"45","author":"Jeon","year":"2011","journal-title":"Atmos. Environ."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1021\/es4048472","article-title":"Inhalable microorganisms in Beijing\u2019s PM2.5 and PM10 pollutants during a severe smog event","volume":"48","author":"Cao","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.atmosenv.2015.09.070","article-title":"Characteristics of bacterial and fungal aerosols during the autumn haze days in Xi\u2019an, China","volume":"122","author":"Li","year":"2015","journal-title":"Atmos. Environ."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.atmosenv.2015.08.004","article-title":"Seasonal size distribution of airborne culturable bacteria and fungi and preliminary estimation of their deposition in human lungs during non-haze and haze days","volume":"118","author":"Gao","year":"2015","journal-title":"Atmos. Environ."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.scitotenv.2015.10.001","article-title":"Concentration and size distribution of total airborne microbes in hazy and foggy weather","volume":"541","author":"Dong","year":"2015","journal-title":"Sci. Total Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1097\/EDE.0b013e318176bfcd","article-title":"Heat effects on mortality in 15 European cities","volume":"19","author":"Baccini","year":"2008","journal-title":"Epidemiology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3184\/003685003783238699","article-title":"Lethal effects of heat on bacterial physiology and structure","volume":"86","author":"Russell","year":"2003","journal-title":"Sci. Prog."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4723","DOI":"10.1016\/j.scitotenv.2009.05.008","article-title":"Thermal effects on bacterial bioaerosols in continuous air flow","volume":"407","author":"Jung","year":"2009","journal-title":"Sci. Total Environ."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.envint.2018.01.007","article-title":"A review on airborne microorganisms in particulate matters: Composition, characteristics and influence factors","volume":"113","author":"Zhai","year":"2018","journal-title":"Environ. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.scitotenv.2017.05.049","article-title":"Meteorological factors had more impact on airborne bacterial communities than air pollutants","volume":"601\u2013602","author":"Zhen","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s10453-018-9510-1","article-title":"Influence of meteorological factors on the level and characteristics of culturable bacteria in the air in Gliwice, Upper Silesia (Poland)","volume":"34","author":"Pastuszka","year":"2018","journal-title":"Aerobiologia"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s002489900046","article-title":"Survey of culturable airborne bacteria at four diverse locations in Oregon: Urban, rural, forest, and coastal","volume":"34","author":"Shaffer","year":"1997","journal-title":"Microb. Ecol."},{"key":"ref_35","first-page":"1","article-title":"Effects of temperature and relative humidity on the survival of airborne bacteria","volume":"864","author":"Hoeksma","year":"2015","journal-title":"Livest. Res. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.15666\/aeer\/0302_139149","article-title":"Assessment of microbial (bacteria) concentrations of ambient air at semi-arid urban region: Influence of meteorological factors","volume":"3","author":"Mouli","year":"2005","journal-title":"Appl. Ecol. Environ. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1111\/j.1751-1097.1997.tb01884.x","article-title":"A study of the relationship between pigmented outdoor atmospheric bacteria and solar radiation","volume":"65","author":"Tong","year":"1997","journal-title":"Photochem. Photobiol."},{"key":"ref_38","unstructured":"Hurst, A., and Nasim, A. (1984). Reversible freeze-injury. Repairable Lesions in Microorganisms, Academic Press."},{"key":"ref_39","unstructured":"Neidhardt, F.C., Ingraham, J.L., and Schaechter, M. (1990). Physiology of the Bacterial Cell: A Molecular Approach: Sinauer Associates Sunderland, Sinauer Associates Inc."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Tringe, S.G., Zhang, T., Liu, X., Yu, Y., Lee, W.H., Yap, J., Yao, F., Suan, S.T., Ing, S.K., and Haynes, M. (2008). The airborne metagenome in an indoor urban environment. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0001862"},{"key":"ref_41","first-page":"249","article-title":"A short overview of the microbial population in clouds: Potential roles in atmospheric chemistry and nucleation processes","volume":"98","author":"Delort","year":"2010","journal-title":"Atmos. Environ."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.jbiosc.2014.10.015","article-title":"Bioprocess of Kosa bioaerosols: Effect of ultraviolet radiation on airborne bacteria within Kosa (Asian dust)","volume":"119","author":"Kobayashi","year":"2015","journal-title":"J. Biosci. Bioeng."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1264\/jsme2.ME13080","article-title":"Changes in the airborne bacterial community in outdoor environments following Asian dust events","volume":"29","author":"Yamaguchi","year":"2014","journal-title":"Microbes Environ."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1098\/rsif.2009.0227.focus","article-title":"The effects of environmental parameters on the survival of airborne infectious agents","volume":"6","author":"Tang","year":"2009","journal-title":"J. R. Soc. Interface"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1007\/s00484-011-0514-6","article-title":"Meteorological factors and ambient bacterial levels in a subtropical urban environment","volume":"56","author":"Wu","year":"2012","journal-title":"Int. J. Biometeorol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/1352-2310(95)00143-M","article-title":"Atmospheric pollution by airborne microorganisms in the city of Marseilles","volume":"30","author":"Krempff","year":"1996","journal-title":"Atmos. Environ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1080\/00039896.1991.9937425","article-title":"A two-year study of microbiological indoor air quality in a new apartment","volume":"46","author":"Macher","year":"1991","journal-title":"Arch. Occup. Environ. Health"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.envpol.2017.10.070","article-title":"Effect of air pollution on the total bacteria and pathogenic bacteria in different sizes of particulate matter","volume":"233","author":"Liu","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1139\/m78-100","article-title":"Effect of sunlight on bacterial survival in transparent air samplers","volume":"24","author":"Fedorak","year":"1978","journal-title":"Can. J. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.atmosenv.2013.12.038","article-title":"Transport of bacterial cells toward the Pacific in northern hemisphere westerly winds","volume":"87","author":"Murata","year":"2014","journal-title":"Atmos. Environ."}],"container-title":["International Journal of Environmental Research and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1660-4601\/15\/10\/2295\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:26:39Z","timestamp":1760196399000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1660-4601\/15\/10\/2295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,19]]},"references-count":50,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2018,10]]}},"alternative-id":["ijerph15102295"],"URL":"https:\/\/doi.org\/10.3390\/ijerph15102295","relation":{},"ISSN":["1660-4601"],"issn-type":[{"value":"1660-4601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,19]]}}}